Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Galpha(14) links a variety of G(i)- and G(s)-coupled receptors to the stimulation of phospholipase C.
Ho MK
,
Yung LY
,
Chan JS
,
Chan JH
,
Wong CS
,
Wong YH
.
???displayArticle.abstract???
1. The bovine Galpha(14) is a member of the G(q) subfamily of G proteins that can regulate phospholipase Cbeta isoforms but the extent to which Galpha(14) recognizes different receptor classes is not known. 2. Galpha(14) was cotransfected with a variety of receptors in COS-7 cells, and agonist-induced stimulation of phospholipase C was then measured. 3. Activation of the type 2 but not type 1 somatostatin receptor in cells coexpressing Galpha(14) stimulated the accumulation of inositol phosphates; functional expression of both subtypes of somatostatin receptors was determined by the ability of somatostatin to inhibit cyclic AMP accumulation. 4. Among the three opioid receptors (mu, delta, and kappa), only the delta receptor was capable of stimulating IP formation when coexpressed with Galpha(14) in COS-7 cells. 5. A panel of G(i)- and G(s)-linked receptors was screened for their ability to stimulate IP accumulation via Galpha(14). The adenosine A(1), complement C5a, dopamine D(1), D(2) and D(5), formyl peptide, luteinizing hormone, secretin, and the three subtypes of melatonin (mt1, MT2, and Xenopus) receptors were all incapable of activating Galpha(14), while the alpha(2)- and beta(2)-adrenoceptors were able to do so. 6. Galpha(14)-mediated stimulation of phospholipase Cbeta was agonist dose-dependent. These data demonstrate that although Galpha(14) can interact with different classes of receptors, it is much less promiscuous than Galpha(15) or Galpha(16).
Akbar,
Phospholipase C activation and Ca2+ mobilization by cloned human somatostatin receptor subtypes 1-5, in transfected COS-7 cells.
1994, Pubmed
Akbar,
Phospholipase C activation and Ca2+ mobilization by cloned human somatostatin receptor subtypes 1-5, in transfected COS-7 cells.
1994,
Pubmed
Amatruda,
G alpha 16, a G protein alpha subunit specifically expressed in hematopoietic cells.
1991,
Pubmed
Barnes,
Effect of beta-agonists on inflammatory cells.
1999,
Pubmed
Bourne,
The GTPase superfamily: a conserved switch for diverse cell functions.
1990,
Pubmed
Buhl,
Selective coupling of the human anaphylatoxin C5a receptor and alpha 16 in human kidney 293 cells.
1993,
Pubmed
Chan,
Activation of type II adenylyl cyclase by the cloned mu-opioid receptor: coupling to multiple G proteins.
1995,
Pubmed
Chan,
Preactivation permits subsequent stimulation of phospholipase C by G(i)-coupled receptors.
2000,
Pubmed
Chen,
Both overlapping and distinct signaling pathways for somatostatin receptor subtypes SSTR1 and SSTR2 in pituitary cells.
1997,
Pubmed
Conklin,
Recombinant Gq alpha. Mutational activation and coupling to receptors and phospholipase C.
1992,
Pubmed
Conklin,
Substitution of three amino acids switches receptor specificity of Gq alpha to that of Gi alpha.
1993,
Pubmed
Daaka,
Switching of the coupling of the beta2-adrenergic receptor to different G proteins by protein kinase A.
1997,
Pubmed
Dippel,
A heterotrimeric G protein complex couples the muscarinic m1 receptor to phospholipase C-beta.
1996,
Pubmed
Elliott,
SSTR2A is the dominant somatostatin receptor subtype expressed by inflammatory cells, is widely expressed and directly regulates T cell IFN-gamma release.
1999,
Pubmed
Garcia,
Pituitary cell line GH3 expresses two somatostatin receptor subtypes that inhibit adenylyl cyclase: functional expression of rat somatostatin receptor subtypes 1 and 2 in human embryonic kidney 293 cells.
1994,
Pubmed
Haskó,
Regulation of cytokine and chemokine production by transmitters and co-transmitters of the autonomic nervous system.
1998,
Pubmed
House,
Suppression of immune function by non-peptidic delta opioid receptor antagonists.
1995,
Pubmed
Komatsuzaki,
A novel system that reports the G-proteins linked to a given receptor: a study of type 3 somatostatin receptor.
1997,
Pubmed
Kostenis,
The N-terminal extension of Galphaq is critical for constraining the selectivity of receptor coupling.
1997,
Pubmed
Kostenis,
Genetic analysis of receptor-Galphaq coupling selectivity.
1997,
Pubmed
Kostenis,
Functional characterization of a series of mutant G protein alphaq subunits displaying promiscuous receptor coupling properties.
1998,
Pubmed
Kuang,
Selective G protein coupling by C-C chemokine receptors.
1996,
Pubmed
Kühn,
G proteins of the Gq family couple the H2 histamine receptor to phospholipase C.
1996,
Pubmed
Lai,
Gz coupling to the rat kappa-opioid receptor.
1995,
Pubmed
,
Xenbase
Lee,
Members of the Gq alpha subunit gene family activate phospholipase C beta isozymes.
1992,
Pubmed
Lee,
Differential coupling of mu-, delta-, and kappa-opioid receptors to G alpha16-mediated stimulation of phospholipase C.
1998,
Pubmed
Lee,
Multiple regions of G alpha 16 contribute to the specificity of activation by the C5a receptor.
1995,
Pubmed
Maeda,
Cell-specific signal transduction of parathyroid hormone (PTH)-related protein through stably expressed recombinant PTH/PTHrP receptors in vascular smooth muscle cells.
1996,
Pubmed
McLaughlin,
Molecular cloning of G proteins and phosphodiesterases from rat taste cells.
1994,
Pubmed
Mody,
Incorporation of Galpha(z)-specific sequence at the carboxyl terminus increases the promiscuity of galpha(16) toward G(i)-coupled receptors.
2000,
Pubmed
Nakamura,
Identification of two novel GTP-binding protein alpha-subunits that lack apparent ADP-ribosylation sites for pertussis toxin.
1991,
Pubmed
Nakamura,
G protein-mediated inhibition of phosphoinositide metabolism evoked by metabotropic glutamate receptors in frog oocytes.
1994,
Pubmed
,
Xenbase
Nakamura,
Characterization of Gq family G proteins GL1 alpha (G14 alpha), GL2 alpha (G11 alpha), and Gq alpha expressed in the baculovirus-insect cell system.
1995,
Pubmed
Offermanns,
G alpha 15 and G alpha 16 couple a wide variety of receptors to phospholipase C.
1995,
Pubmed
Roy,
Effects of opioids on the immune system.
1996,
Pubmed
Simon,
Diversity of G proteins in signal transduction.
1991,
Pubmed
Strathmann,
G protein diversity: a distinct class of alpha subunits is present in vertebrates and invertebrates.
1990,
Pubmed
Tsu,
Regulation of multiple effectors by the cloned delta-opioid receptor: stimulation of phospholipase C and type II adenylyl cyclase.
1995,
Pubmed
Wilkie,
Characterization of G-protein alpha subunits in the Gq class: expression in murine tissues and in stromal and hematopoietic cell lines.
1991,
Pubmed
Wong,
Gi assays in transfected cells.
1994,
Pubmed
Wu,
Activation of phospholipase C by alpha 1-adrenergic receptors is mediated by the alpha subunits of Gq family.
1992,
Pubmed
Wu,
G protein-coupled signal transduction pathways for interleukin-8.
1993,
Pubmed
Wu,
Different alpha 1-adrenergic receptor sequences required for activating different G alpha subunits of Gq class of G proteins.
1995,
Pubmed
Wu,
Selective coupling of beta 2-adrenergic receptor to hematopoietic-specific G proteins.
1995,
Pubmed
Xie,
Two basic amino acids in the second inner loop of the interleukin-8 receptor are essential for Galpha16 coupling.
1997,
Pubmed
Yung,
GalphaL1 (Galpha14) couples the opioid receptor-like1 receptor to stimulation of phospholipase C.
1999,
Pubmed
Zhu,
G protein subunits and the stimulation of phospholipase C by Gs-and Gi-coupled receptors: Lack of receptor selectivity of Galpha(16) and evidence for a synergic interaction between Gbeta gamma and the alpha subunit of a receptor activated G protein.
1996,
Pubmed
Zigman,
Expression of cone transducin, Gz alpha, and other G-protein alpha-subunit messenger ribonucleic acids in pancreatic islets.
1994,
Pubmed