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EMBO Rep
2001 Mar 01;23:217-21. doi: 10.1093/embo-reports/kve040.
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Cleavage of non-tRNA substrates by eukaryal tRNA splicing endonucleases.
Fruscoloni P
,
Baldi MI
,
Tocchini-Valentini GP
.
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Eukaryal tRNA splicing endonucleases use the mature domains of pre-tRNAs as their primary recognition elements. However, they can also cleave in a mode that is independent of the mature domain, when substrates are able to form the bulge-helix-bulge structure (BHB), which is cleaved by archaeal tRNA endonucleases. We present evidence that the eukaryal enzymes cleave their substrates after forming a structure that resembles the BHB. Consequently, these enzymes can cleave substrates that lack the mature domain altogether. That raises the possibility that these enzymes could also cleave non-tRNA substrates that already have a BHB. As predicted, they can do so, both in vitro and in vivo.
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