Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Molecular and functional characterization of a novel gonadotropin-releasing-hormone receptor isolated from the common octopus (Octopus vulgaris).
Kanda A
,
Takahashi T
,
Satake H
,
Minakata H
.
???displayArticle.abstract??? GnRH (gonadotropin-releasing hormone) plays a pivotal role in the regulation of reproduction in vertebrates through interaction with a specific receptor. Previously, we isolated a GnRH homologue, oct-GnRH, from the common octopus (Octopus vulgaris). In the present study, we have identified a GnRH receptor (oct-GnRHR) specific for oct-GnRH from Octopus brain. Oct-GnRHR includes domains and motifs typical of vertebrate GnRH receptors. The intron-inserted positions are conserved between oct-GnRHR and the chordate GnRHR genes. The oct-GnRHR expressed in Xenopus (South African clawed frog) oocytes was responsive to oct-GnRH, but not to any other HPLC fractions of the Octopus brain extract. These results show that oct-GnRHR is an authentic receptor for oct-GnRH. Southern blotting of reverse-transcription PCR products revealed that the oct-GnRHR mRNA was widely distributed in the central and peripheral nervous systems and in several peripheral tissues. In situ hybridization showed that oct-GnRHR mRNA was expressed in some regions involved in autonomic functions, feeding, memory and movement. Oct-GnRH was shown to induce steroidogenesis of testosterone, progesterone and 17beta-oestradiol in Octopus ovary and testis, where oct-GnRHR was abundantly expressed. These results suggest that oct-GnRH, like its vertebrate counterparts, acts as a multifunctional neurotransmitter, neuromodulator and hormone-like factor, both in Octopus central nervous system and peripheral tissues, and that both structure and functions of the GnRH family are, at least partially, evolutionarily conserved between octopuses and chordates.
Arora,
Dependence of agonist activation on an aromatic moiety in the DPLIY motif of the gonadotropin-releasing hormone receptor.
1996, Pubmed
Arora,
Dependence of agonist activation on an aromatic moiety in the DPLIY motif of the gonadotropin-releasing hormone receptor.
1996,
Pubmed
Ballesteros,
Functional microdomains in G-protein-coupled receptors. The conserved arginine-cage motif in the gonadotropin-releasing hormone receptor.
1998,
Pubmed
Bockaert,
Molecular tinkering of G protein-coupled receptors: an evolutionary success.
1999,
Pubmed
Cheng,
Molecular biology of gonadotropin-releasing hormone (GnRH)-I, GnRH-II, and their receptors in humans.
2005,
Pubmed
Cui,
Identification of Phe313 of the gonadotropin-releasing hormone (GnRH) receptor as a site critical for the binding of nonpeptide GnRH antagonists.
2000,
Pubmed
D'Aniello,
Occurrence of sex steroid hormones and their binding proteins in Octopus vulgaris lam.
1996,
Pubmed
Di Cosmo,
A estradiol-17beta receptor in the reproductive system of the female of Octopus vulgaris: characterization and immunolocalization.
2002,
Pubmed
Di Cosmo,
Progesterone receptor in the reproductive system of the female of Octopus vulgaris: characterization and immunolocalization.
1998,
Pubmed
Di Cosmo,
Neuropeptidergic control of the optic gland of Octopus vulgaris: FMRF-amide and GnRH immunoreactivity.
1998,
Pubmed
Di Cosmo,
Sex steroid hormone fluctuations and morphological changes of the reproductive system of the female of Octopus vulgaris throughout the annual cycle.
2001,
Pubmed
Di Cristo,
Presence of two neuropeptides in the fusiform ganglion and reproductive ducts of Octopus vulgaris: FMRFamide and gonadotropin-releasing hormone (GnRH).
2002,
Pubmed
Iwakoshi,
Isolation and characterization of a GnRH-like peptide from Octopus vulgaris.
2002,
Pubmed
Iwakoshi-Ukena,
Expression and distribution of octopus gonadotropin-releasing hormone in the central nervous system and peripheral organs of the octopus (Octopus vulgaris) by in situ hybridization and immunohistochemistry.
2004,
Pubmed
Kakar,
Cloning, sequencing, and expression of human gonadotropin releasing hormone (GnRH) receptor.
1992,
Pubmed
Kanda,
Cloning of Octopus cephalotocin receptor, a member of the oxytocin/vasopressin superfamily.
2003,
Pubmed
,
Xenbase
Kanda,
Novel evolutionary lineages of the invertebrate oxytocin/vasopressin superfamily peptides and their receptors in the common octopus (Octopus vulgaris).
2005,
Pubmed
,
Xenbase
Kobayashi,
Modulatory actions of octopamine and serotonin on the contraction of buccal muscles in Rapana thomasiana--I. Enhancement of contraction in radula protractor.
1980,
Pubmed
Kusakabe,
Structure, expression, and cluster organization of genes encoding gonadotropin-releasing hormone receptors found in the neural complex of the ascidian Ciona intestinalis.
2003,
Pubmed
,
Xenbase
Leung,
Intracellular signaling in the gonads.
1992,
Pubmed
Leung,
Gonadotropin-releasing hormone receptor: gene structure, expression and regulation.
1996,
Pubmed
Millar,
Gonadotropin-releasing hormone receptors.
2004,
Pubmed
Morgan,
Evolution of GnRH ligand precursors and GnRH receptors in protochordate and vertebrate species.
2004,
Pubmed
Okubo,
Identification and characterization of two distinct GnRH receptor subtypes in a teleost, the medaka Oryzias latipes.
2001,
Pubmed
Rodet,
Molecular cloning of a molluscan gonadotropin-releasing hormone receptor orthologue specifically expressed in the gonad.
2005,
Pubmed
Satake,
Evidence for conservation of the vasopressin/oxytocin superfamily in Annelida.
1999,
Pubmed
Staubli,
Molecular identification of the insect adipokinetic hormone receptors.
2002,
Pubmed
Takuwa-Kuroda,
Octopus, which owns the most advanced brain in invertebrates, has two members of vasopressin/oxytocin superfamily as in vertebrates.
2003,
Pubmed
Tosti,
Progesterone induces activation in Octopus vulgaris spermatozoa.
2001,
Pubmed
Troskie,
Complementary deoxyribonucleic acid cloning, gene expression, and ligand selectivity of a novel gonadotropin-releasing hormone receptor expressed in the pituitary and midbrain of Xenopus laevis.
2000,
Pubmed
,
Xenbase
Wang,
Three distinct types of GnRH receptor characterized in the bullfrog.
2001,
Pubmed
,
Xenbase