Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
XB-ART-8867
Sheng Wu Gong Cheng Xue Bao 2001 Mar 01;172:207-10.
Show Gene links Show Anatomy links

[Cloning and expression of a synthetic gene encoding magainin-melittin hybrid peptide in Escherichia coli and studies on its antibacterial activity].

Huang Y , Liu FP , Zhou TH , Zhu JM .


???displayArticle.abstract???
A hybrid peptide gene was designed and synthesized. Its encoding peptide is constructed from residues 3-14 of magainin and residues 1-13 of melittin. The MA-E gene was cloned into plasmids pUC18 and pBV220. By DNA sequencing, the whole sequences of this gene is confirmed to be correct. The recombinant plasmid pBMA-E was expressed in E. coli DH5 alpha. A gene product band can be seen with Tricine-SDS-PAGE. The MA-E hybrid peptide was purified by immobilized metal affinity chromatography. Bioactivity assay was carried out in liquid turbidity method. The bactericide value to E. coli K12D31 is 0.182.

???displayArticle.pubmedLink??? 11411233



Species referenced: Xenopus
Genes referenced: acta4 magainins