Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
ADP-ribosylation of Rab5 by ExoS of Pseudomonas aeruginosa affects endocytosis.
Barbieri AM
,
Sha Q
,
Bette-Bobillo P
,
Stahl PD
,
Vidal M
.
???displayArticle.abstract???
Pseudomonas aeruginosa exoenzyme S (ExoS) is an ADP-ribosyltransferase that modifies low-molecular-weight GTPases. Here we studied the effect of Rab5 ADP-ribosylation by ExoS on its cellular function, i.e., regulation of early endocytic events. Coculture of CHO cells with P. aeruginosa induced a marked decrease in horseradish peroxidase (HRP) uptake compared to noninfected cells, while coculture with a P. aeruginosa mutant strain that fails to produce ExoS did not lead to any change in HRP uptake. Microinjection of recombinant ExoS into Xenopus oocytes induced strong inhibition of basal HRP uptake by oocytes. Moreover, coinjection of recombinant ExoS with Rab5 abolished the typical stimulation of HRP uptake obtained after GTPase microinjection. Cytosols prepared from injected oocytes were used in an endosome-endosome fusion assay. Cytosol from ExoS-microinjected oocytes was ineffective in promoting endosome-endosome fusion. However, in these conditions, the addition of Rab5 to the assay led to fusion recovery. Finally, we found that the interaction of Rab5 with EEA1 was markedly diminished after Rab5 ADP-ribosylation by ExoS.
Alvarez-Dominguez,
Increased expression of Rab5a correlates directly with accelerated maturation of Listeria monocytogenes phagosomes.
1999, Pubmed
Alvarez-Dominguez,
Increased expression of Rab5a correlates directly with accelerated maturation of Listeria monocytogenes phagosomes.
1999,
Pubmed
Barbieri,
Rab5, an early acting endosomal GTPase, supports in vitro endosome fusion without GTP hydrolysis.
1994,
Pubmed
Barbieri,
Evidence for a symmetrical requirement for Rab5-GTP in in vitro endosome-endosome fusion.
1998,
Pubmed
Bette-Bobillo,
Exoenzyme S from P. aeruginosa ADP ribosylates rab4 and inhibits transferrin recycling in SLO-permeabilized reticulocytes.
1998,
Pubmed
Bodey,
Infections caused by Pseudomonas aeruginosa.
1983,
Pubmed
Christoforidis,
The Rab5 effector EEA1 is a core component of endosome docking.
1999,
Pubmed
Christoforidis,
Phosphatidylinositol-3-OH kinases are Rab5 effectors.
1999,
Pubmed
Coburn,
ADP-ribosylation of p21ras and related proteins by Pseudomonas aeruginosa exoenzyme S.
1991,
Pubmed
Diaz,
Vesicle fusion following receptor-mediated endocytosis requires a protein active in Golgi transport.
1989,
Pubmed
Dorner,
The kinesin-like motor protein KIF1C occurs in intact cells as a dimer and associates with proteins of the 14-3-3 family.
1999,
Pubmed
Fu,
The eukaryotic host factor that activates exoenzyme S of Pseudomonas aeruginosa is a member of the 14-3-3 protein family.
1993,
Pubmed
Ganesan,
Pseudomonas aeruginosa exoenzyme S disrupts Ras-mediated signal transduction by inhibiting guanine nucleotide exchange factor-catalyzed nucleotide exchange.
1999,
Pubmed
Ganesan,
Pseudomonas aeruginosa exoenzyme S ADP-ribosylates Ras at multiple sites.
1998,
Pubmed
Gelperin,
14-3-3 proteins: potential roles in vesicular transport and Ras signaling in Saccharomyces cerevisiae.
1995,
Pubmed
Goehring,
The N-terminal domain of Pseudomonas aeruginosa exoenzyme S is a GTPase-activating protein for Rho GTPases.
1999,
Pubmed
Horiuchi,
A novel Rab5 GDP/GTP exchange factor complexed to Rabaptin-5 links nucleotide exchange to effector recruitment and function.
1997,
Pubmed
Knight,
Functional domains of Pseudomonas aeruginosa exoenzyme S.
1995,
Pubmed
Kulich,
Expression of recombinant exoenzyme S of Pseudomonas aeruginosa.
1995,
Pubmed
Li,
Structure-function relationship of the small GTPase rab5.
1993,
Pubmed
Li,
Evidence for phosphatidylinositol 3-kinase as a regulator of endocytosis via activation of Rab5.
1995,
Pubmed
Masters,
Interaction of 14-3-3 with a nonphosphorylated protein ligand, exoenzyme S of Pseudomonas aeruginosa.
1999,
Pubmed
McGuffie,
Modification of Ras in eukaryotic cells by Pseudomonas aeruginosa exoenzyme S.
1998,
Pubmed
McGuffie,
Differential sensitivity of human epithelial cells to Pseudomonas aeruginosa exoenzyme S.
1999,
Pubmed
Mukhopadhyay,
Sequential actions of Rab5 and Rab7 regulate endocytosis in the Xenopus oocyte.
1997,
Pubmed
,
Xenbase
Roth,
Characterization of 14-3-3 proteins in adrenal chromaffin cells and demonstration of isoform-specific phospholipid binding.
1994,
Pubmed
,
Xenbase
Simonsen,
EEA1 links PI(3)K function to Rab5 regulation of endosome fusion.
1998,
Pubmed
Vidal,
The small GTP-binding proteins Rab4 and ARF are associated with released exosomes during reticulocyte maturation.
1993,
Pubmed
Yahr,
Exoenzyme S of Pseudomonas aeruginosa is secreted by a type III pathway.
1996,
Pubmed
Zhang,
Use of biotinylated NAD to label and purify ADP-ribosylated proteins.
1997,
Pubmed
Zhang,
Residues of 14-3-3 zeta required for activation of exoenzyme S of Pseudomonas aeruginosa.
1999,
Pubmed