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Br J Pharmacol
2001 Nov 01;1346:1195-206. doi: 10.1038/sj.bjp.0704361.
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Characterization of two Bunodosoma granulifera toxins active on cardiac sodium channels.
Goudet C
,
Ferrer T
,
Galàn L
,
Artiles A
,
Batista CF
,
Possani LD
,
Alvarez J
,
Aneiros A
,
Tytgat J
.
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1. Two sodium channel toxins, BgII and BgIII, have been isolated and purified from the sea anemone Bunodosoma granulifera. Combining different techniques, we have investigated the electrophysiological properties of these toxins. 2. We examined the effect of BgII and BgIII on rat ventricular strips. These toxins prolong action potentials with EC50 values of 60 and 660 nM and modify the resting potentials. 3. The effect on Na+ currents in rat cardiomyocytes was studied using the patch-clamp technique. BgII and BgIII slow the rapid inactivation process and increase the current density with EC50 values of 58 and 78 nM, respectively. 4. On the cloned hH1 cardiac Na+ channel expressed in Xenopus laevis oocytes, BgII and BgIII slow the inactivation process of Na+ currents (respective EC50 values of 0.38 and 7.8 microM), shift the steady-state activation and inactivation parameters to more positive potentials and the reversal potential to more negative potentials. 5. The amino acid sequences of these toxins are almost identical except for an asparagine at position 16 in BgII which is replaced by an aspartic acid in BgIII. In all experiments, BgII was more potent than BgIII suggesting that this conservative residue is important for the toxicity of sea anemone toxins. 6. We conclude that BgII and BgIII, generally known as neurotoxins, are also cardiotoxic and combine the classical effects of sea anemone Na+ channels toxins (slowing of inactivation kinetics, shift of steady-state activation and inactivation parameters) with a striking decrease on the ionic selectivity of Na+ channels.
Alsen,
Mechanical and electrophysiological effects of sea anemone (Anemonia sulcata) toxins on rat innervated and denervated skeletal muscle.
1981, Pubmed
Alsen,
Mechanical and electrophysiological effects of sea anemone (Anemonia sulcata) toxins on rat innervated and denervated skeletal muscle.
1981,
Pubmed
Alvarez,
Changes in electrical and mechanical activities of rabbit papillary muscle during hypoxic perfusion.
1981,
Pubmed
Aneiros,
A potassium channel toxin from the secretion of the sea anemone Bunodosoma granulifera. Isolation, amino acid sequence and biological activity.
1993,
Pubmed
Balser,
Structure and function of the cardiac sodium channels.
1999,
Pubmed
Benoit,
Properties of maintained sodium current induced by a toxin from Androctonus scorpion in frog node of Ranvier.
1987,
Pubmed
Benzinger,
Differences in the binding sites of two site-3 sodium channel toxins.
1997,
Pubmed
Bruhn,
Isolation and characterisation of five neurotoxic and cardiotoxic polypeptides from the sea anemone Anthopleura elegantissima.
2001,
Pubmed
Catterall,
Structure and function of voltage-gated ion channels.
1995,
Pubmed
Catterall,
From ionic currents to molecular mechanisms: the structure and function of voltage-gated sodium channels.
2000,
Pubmed
Cestèle,
Molecular mechanisms of neurotoxin action on voltage-gated sodium channels.
2000,
Pubmed
Chahine,
Sea anemone toxin (ATX II) modulation of heart and skeletal muscle sodium channel alpha-subunits expressed in tsA201 cells.
1996,
Pubmed
Chiamvimonvat,
Control of ion flux and selectivity by negatively charged residues in the outer mouth of rat sodium channels.
1996,
Pubmed
,
Xenbase
Dascal,
The use of Xenopus oocytes for the study of ion channels.
1987,
Pubmed
,
Xenbase
Denac,
Structure, function and pharmacology of voltage-gated sodium channels.
2000,
Pubmed
Dongré,
Emerging tandem-mass-spectrometry techniques for the rapid identification of proteins.
1997,
Pubmed
Galán,
Characteristics of Ca2+ channel blockade by oxodipine and elgodipine in rat cardiomyocytes.
1998,
Pubmed
Gellens,
Primary structure and functional expression of the human cardiac tetrodotoxin-insensitive voltage-dependent sodium channel.
1992,
Pubmed
,
Xenbase
Goldin,
Nomenclature of voltage-gated sodium channels.
2000,
Pubmed
Hamill,
Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches.
1981,
Pubmed
Kem,
Isolation, characterization, and amino acid sequence of a polypeptide neurotoxin occurring in the sea anemone Stichodactyla helianthus.
1989,
Pubmed
Kudo,
The potent excitatory effect of a novel polypeptide, anthopleurin-B, isolated from a sea anemone (Anthopleura xanthogrammica) on the frog spinal cord.
1980,
Pubmed
Lazdunski,
Polypeptide toxins as tools to study voltage-sensitive Na+ channels.
1986,
Pubmed
Liman,
Subunit stoichiometry of a mammalian K+ channel determined by construction of multimeric cDNAs.
1992,
Pubmed
,
Xenbase
Loret,
Positively charged amino acid residues located similarly in sea anemone and scorpion toxins.
1994,
Pubmed
Ménez,
Functional architectures of animal toxins: a clue to drug design?
1998,
Pubmed
Nicholson,
Modification of sodium channel gating and kinetics by versutoxin from the Australian funnel-web spider Hadronyche versuta.
1994,
Pubmed
Nicholson,
Characterisation of the effects of robustoxin, the lethal neurotoxin from the Sydney funnel-web spider Atrax robustus, on sodium channel activation and inactivation.
1998,
Pubmed
Norton,
Cardiotonic polypeptides from Anthopleura xanthogrammica (Brandt) and A. elegantissima (Brandt).
1981,
Pubmed
Norton,
Structure and structure-function relationships of sea anemone proteins that interact with the sodium channel.
1991,
Pubmed
Possani,
Scorpion toxins specific for Na+-channels.
1999,
Pubmed
Ravens,
Electromechanical studies of an Anemonia sulcata toxin in mammalian cardiac muscle.
1976,
Pubmed
Reimer,
Amino acid sequence of the Anthopleura xanthogrammica heart stimulant, anthopleurin-B.
1985,
Pubmed
Renaud,
The interaction of polypeptide neurotoxins with tetrodotoxin-resistant Na+ channels in mammalian cardiac cells. Correlation with inotropic and arrhythmic effects.
1986,
Pubmed
Rogers,
Molecular determinants of high affinity binding of alpha-scorpion toxin and sea anemone toxin in the S3-S4 extracellular loop in domain IV of the Na+ channel alpha subunit.
1996,
Pubmed
Salinas,
Effects of a toxin from the mucus of the Caribbean sea anemone (Bunodosoma granulifera) on the ionic currents of single ventricular mammalian cardiomyocytes.
1997,
Pubmed
Schweitz,
Purification and pharmacological properties of eight sea anemone toxins from Anemonia sulcata, Anthopleura xanthogrammica, Stoichactis giganteus, and Actinodendron plumosum.
1981,
Pubmed
Shibata,
A polypeptide (AP-A) from sea anemone (Anthopleura xanthogrammica) with potent positive inotropic action.
1976,
Pubmed
Tanaka,
Amino acid sequence of the Anthopleura xanthogrammica heart stimulant, anthopleurin A.
1977,
Pubmed
Ulbricht,
Modification of sodium channels in myelinated nerve by Anemonia sulcata toxin II.
1981,
Pubmed
Wilcox,
Refined structure in solution of the sea anemone neurotoxin ShI.
1993,
Pubmed
Wunderer,
Amino-acid sequence of a coelenterate toxin: toxin II from Anemonia sulcata.
1976,
Pubmed
Wunderer,
Amino-acid sequence of toxin I from Anemonia sulcata.
1978,
Pubmed
