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Spark- and ember-like elementary Ca2+ release events in skinned fibres of adult mammalian skeletal muscle.
Kirsch WG
,
Uttenweiler D
,
Fink RH
.
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1. Using laser scanning confocal microscopy, we show for the first time elementary Ca2+ release events (ECRE) from the sarcoplasmic reticulum in chemically and mechanically skinned fibres from adult mammalian muscle and compare them with ECRE from amphibian skinned fibres. 2. Hundreds of spontaneously occurring events could be measured from individual single skinned mammalian fibres. In addition to spark-like events, we found ember-like events, i.e. long-lasting events of steady amplitude. These two different fundamental release types in mammalian muscle could occur in combination at the same location. 3. The two peaks of the frequency of occurrence for ECRE of mammalian skeletal muscle coincided with the expected locations of the transverse tubular system within the sarcomere, suggesting that ECRE mainly originate at triadic junctions. 4. ECRE in adult mammalian muscle could also be identified at the onset of the global Ca2+ release evoked by membrane depolarisation in mechanically skinned fibres. In addition, the frequency of ECRE was significantly increased by application of 0.5 mM caffeine and reduced by application of 2 mM tetracaine. 5. We conclude that the excitation-contraction coupling process in adult mammalian muscle involves the activation of both spark- and ember-like elementary Ca2+ release events.
Balshaw,
Calmodulin binding and inhibition of cardiac muscle calcium release channel (ryanodine receptor).
2001, Pubmed
Balshaw,
Calmodulin binding and inhibition of cardiac muscle calcium release channel (ryanodine receptor).
2001,
Pubmed
Block,
Structural evidence for direct interaction between the molecular components of the transverse tubule/sarcoplasmic reticulum junction in skeletal muscle.
1988,
Pubmed
Cheng,
Calcium sparks: elementary events underlying excitation-contraction coupling in heart muscle.
1993,
Pubmed
Conklin,
Comparison of Ca(2+) sparks produced independently by two ryanodine receptor isoforms (type 1 or type 3).
2000,
Pubmed
Conklin,
Contribution of ryanodine receptor type 3 to Ca(2+) sparks in embryonic mouse skeletal muscle.
1999,
Pubmed
Dulhunty,
The voltage-activation of contraction in skeletal muscle.
1992,
Pubmed
Escobar,
Localization of the site of Ca2+ release at the level of a single sarcomere in skeletal muscle fibres.
1994,
Pubmed
Fink,
Ca2+-movements in muscle modulated by the state of K+-channels in the sarcoplasmic reticulum membranes.
1987,
Pubmed
Franzini-Armstrong,
Ryanodine receptors of striated muscles: a complex channel capable of multiple interactions.
1997,
Pubmed
González,
The spark and its ember: separately gated local components of Ca(2+) release in skeletal muscle.
2000,
Pubmed
González,
Involvement of multiple intracellular release channels in calcium sparks of skeletal muscle.
2000,
Pubmed
Györke,
Dual effects of tetracaine on spontaneous calcium release in rat ventricular myocytes.
1997,
Pubmed
Haarmann,
Oxidation and reduction of pig skeletal muscle ryanodine receptors.
1999,
Pubmed
Herrmann-Frank,
Caffeine and excitation-contraction coupling in skeletal muscle: a stimulating story.
1999,
Pubmed
Klein,
Two mechanisms of quantized calcium release in skeletal muscle.
1996,
Pubmed
Lacampagne,
Modulation of the frequency of spontaneous sarcoplasmic reticulum Ca2+ release events (Ca2+ sparks) by myoplasmic [Mg2+] in frog skeletal muscle.
1998,
Pubmed
Lamb,
Calcium release in skinned muscle fibres of the toad by transverse tubule depolarization or by direct stimulation.
1990,
Pubmed
Lamb,
Excitation-contraction coupling in skeletal muscle: comparisons with cardiac muscle.
2000,
Pubmed
Lipp,
Fundamental calcium release events revealed by two-photon excitation photolysis of caged calcium in Guinea-pig cardiac myocytes.
1998,
Pubmed
Nelson,
Relaxation of arterial smooth muscle by calcium sparks.
1995,
Pubmed
Rios,
Involvement of dihydropyridine receptors in excitation-contraction coupling in skeletal muscle.
,
Pubmed
Shirokova,
Caffeine enhances intramembranous charge movement in frog skeletal muscle by increasing cytoplasmic Ca2+ concentration.
1996,
Pubmed
Shirokova,
Small event Ca2+ release: a probable precursor of Ca2+ sparks in frog skeletal muscle.
1997,
Pubmed
Shirokova,
Local calcium release in mammalian skeletal muscle.
1998,
Pubmed
Shirokova,
Spatially segregated control of Ca2+ release in developing skeletal muscle of mice.
1999,
Pubmed
Smith,
A comparison of the effects of ATP and tetracaine on spontaneous Ca(2+) release from rat permeabilised cardiac myocytes.
2001,
Pubmed
Tsugorka,
Imaging elementary events of calcium release in skeletal muscle cells.
1995,
Pubmed
Uttenweiler,
Mathematical modeling and fluorescence imaging to study the Ca2+ turnover in skinned muscle fibers.
1998,
Pubmed
Ward,
Expression of ryanodine receptor RyR3 produces Ca2+ sparks in dyspedic myotubes.
2000,
Pubmed