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XB-ART-6883
EMBO J 2002 Jul 15;2114:3816-28. doi: 10.1093/emboj/cdf376.
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Site-specific cross-linking analyses reveal an asymmetric protein distribution for a box C/D snoRNP.

Cahill NM , Friend K , Speckmann W , Li ZH , Terns RM , Terns MP , Steitz JA .


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Methylation of the ribose 2'-hydroxyl, the most widespread modification of ribosomal and splicesomal RNAs, is guided by the box C/D class of small nucleolar RNAs (snoRNAs). Box C/D small nucleolar ribonucleoproteins (snoRNPs) contain four core proteins: fibrillarin, Nop56, Nop58 and 15.5 kDa. We constructed U25 snoRNAs containing a single photoactivatable 4-thiouridine at each U position within the conserved box C/D and C'/D' motifs. Proteins assembled on the snoRNA after injection into Xenopus oocyte nuclei were identified by cross-linking, and reconstituted particles characterized by functional rescue and mutational analyses. Our data argue that box C/D snoRNPs are asymmetric, with the C' box contacting Nop56 and fibrillarin, the C box interacting with Nop58, and the D and D' boxes contacting fibrillarin. No cross-link to 15.5 kDa was detected; its binding is disrupted by 4-thiouridine substitution in position 1 of the C box. Repositioning the guide sequence of U25 upstream of box D instead of D' revealed that both C/D motifs have the potential to function as guide centers, but, surprisingly, there was no alteration in protein cross-linking.

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Species referenced: Xenopus
Genes referenced: nop56 nop58

References [+] :
Anderson, Processing of adenovirus 2-induced proteins. 1973, Pubmed