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In Vitro Cell Dev Biol Anim
2002 Apr 01;384:218-27. doi: 10.1290/1071-2690(2002)038<0218:COGTBC>2.0.CO;2.
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Characterization of glucose transport by cultured rabbit kidneyproximal convoluted and proximal straight tubule cells.
Del Valle PL
,
Trifillis A
,
Ruegg CE
,
Kane AS
.
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Rabbit kidneyproximal convoluted tubule (RPCT) and proximal straight tubule (RPST) cells were independently isolated and cultured. The kinetics of the sodium-dependent glucose transport was characterized by determining the uptake of the glucose analog alpha-methylglucopyranoside. Cell culture and assay conditions used in these experiments were based on previous experiments conducted on the renal cell line derived from the whole kidney of the Yorkshire pig (LLC-PK1). Results indicated the presence of two distinct sodium-dependent glucose transporters in rabbit renal cells: a relatively high-capacity, low-affinity transporter (V(max) = 2.28 +/- 0.099 nmoles/mg protein min, Km = 4.1 +/- 0.27 mM) in RPCT cells and a low-capacity, high-affinity transporter (V(max) = 0.45 +/- 0.076 nmoles/mg protein min, K(m) = 1.7 +/- 0.43 mM) in RPST cells. A relatively high-capacity, low-affinity transporter (V(max) = 1.68 +/- 0.215 nmoles/mg protein min, Km = 4.9 +/- 0.23 mM) was characterized in LLC-PK1 cells. Phlorizin inhibited the uptake of alpha-methylglucopyranoside in proximal convoluted, proximal straight, and LLC-PK1 cells by 90, 50, and 90%, respectively. Sodium-dependent glucose transport in all three cell types was specific for hexoses. These data are consistent with the kinetic heterogeneity of sodium-dependent glucose transport in the S1-S2 and S3 segments of the mammalian renal proximal tubule. The RPCT-RPST cultured cell model is novel, and this is the first report of sodium-dependent glucose transport characterization in primary cultures of proximal straight tubule cells. Our results support the use of cultured monolayers of RPCT and RPST cells as a model system to evaluate segment-specific differences in these renal cell types.
Alavi,
Sodium-dependent glucose transport by cultured proximal tubule cells.
1987, Pubmed
Alavi,
Sodium-dependent glucose transport by cultured proximal tubule cells.
1987,
Pubmed
Barfuss,
Differences in active and passive glucose transport along the proximal nephron.
1981,
Pubmed
Bell,
Initiation and characterization of primary mouse kidney epithelial cultures.
1988,
Pubmed
Berry,
Heterogeneity of tubular transport processes in the nephron.
1982,
Pubmed
Blais,
Primary culture of rabbit proximal tubules as a cellular model to study nephrotoxicity of xenobiotics.
1993,
Pubmed
Boogaard,
Primary culture of proximal tubular cells from normal rat kidney as an in vitro model to study mechanisms of nephrotoxicity. Toxicity of nephrotoxicants at low concentrations during prolonged exposure.
1990,
Pubmed
Boogaard,
Isolated proximal tubular cells from rat kidney as an in vitro model for studies on nephrotoxicity. I. An improved method for preparation of proximal tubular cells and their functional characterization by alpha-methylglucose uptake.
1989,
Pubmed
Chung,
Characterization of primary rabbit kidney cultures that express proximal tubule functions in a hormonally defined medium.
1982,
Pubmed
Clancey,
Differential regulation of three glucose transporter genes in a renal epithelial cell line.
2000,
Pubmed
Courjault-Gautier,
Consecutive use of hormonally defined serum-free media to establish highly differentiated human renal proximal tubule cells in primary culture.
1995,
Pubmed
Elfarra,
Renal processing of glutathione conjugates. Role in nephrotoxicity.
1984,
Pubmed
Garvin,
Glucose absorption by isolated perfused rat proximal straight tubules.
1990,
Pubmed
Hediger,
Assignment of the human intestinal Na+/glucose cotransporter gene (SGLT1) to the q11.2----qter region of chromosome 22.
1989,
Pubmed
Hirayama,
Kinetic and specificity differences between rat, human, and rabbit Na+-glucose cotransporters (SGLT-1).
1996,
Pubmed
,
Xenbase
Horio,
Gentamicin inhibits Na+-dependent D-glucose transport in rabbit kidney brush-border membrane vesicles.
1986,
Pubmed
Hull,
The origin and characteristics of a pig kidney cell strain, LLC-PK.
1976,
Pubmed
Ikeda,
Characterization of a Na+/glucose cotransporter cloned from rabbit small intestine.
1989,
Pubmed
,
Xenbase
Kanai,
The human kidney low affinity Na+/glucose cotransporter SGLT2. Delineation of the major renal reabsorptive mechanism for D-glucose.
1994,
Pubmed
,
Xenbase
Kempson,
Proximal tubule characteristics of cultured human renal cortex epithelium.
1989,
Pubmed
Koepsell,
Function and presumed molecular structure of Na(+)-D-glucose cotransport systems.
1994,
Pubmed
Koepsell,
Two substrate sites in the renal Na(+)-D-glucose cotransporter studied by model analysis of phlorizin binding and D-glucose transport measurements.
1990,
Pubmed
Kong,
Cloning and expression of a mammalian Na+/amino acid cotransporter with sequence similarity to Na+/glucose cotransporters.
1993,
Pubmed
Lambotte,
The human gene of a protein that modifies Na(+)-D-glucose co-transport.
1996,
Pubmed
,
Xenbase
Lash,
Susceptibility to toxic injury in different nephron cell populations.
1990,
Pubmed
Lever,
Expression of a differentiated transport function in apical membrane vesicles isolated from an established kidney epithelial cell line. Sodium electrochemical potential-mediated active sugar transport.
1982,
Pubmed
Lever,
Regulation of dome formation in kidney epithelial cell cultures.
1981,
Pubmed
Mackenzie,
Biophysical characteristics of the pig kidney Na+/glucose cotransporter SGLT2 reveal a common mechanism for SGLT1 and SGLT2.
1996,
Pubmed
,
Xenbase
Mackenzie,
SAAT1 is a low affinity Na+/glucose cotransporter and not an amino acid transporter. A reinterpretation.
1994,
Pubmed
,
Xenbase
Misfeldt,
Transepithelial glucose transport in cell culture.
1981,
Pubmed
Moran,
Na+-dependent hexose transport in vesicles from cultured renal epithelial cell line.
1982,
Pubmed
Moran,
Regulation of sodium-coupled glucose transport by glucose in a cultured epithelium.
1983,
Pubmed
Oulianova,
Sugar transport heterogeneity in the kidney: two independent transporters or different transport modes through an oligomeric Protein? 1. Glucose transport studies.
1996,
Pubmed
Pajor,
Molecular evidence for two renal Na+/glucose cotransporters.
1992,
Pubmed
,
Xenbase
Peng,
Polyamine regulation of Na+/glucose symporter expression in LLC-PK1 cells.
1993,
Pubmed
Poppe,
Expression of the Na+-D-glucose cotransporter SGLT1 in neurons.
1997,
Pubmed
Quamme,
Evidence for a high-affinity sodium-dependent D-glucose transport system in the kidney.
1987,
Pubmed
Rabito,
Na+-dependent sugar transport in a cultured epithelial cell line from pig kidney.
1980,
Pubmed
Reinhardt,
Cloning and characterization of the transport modifier RS1 from rabbit which was previously assumed to be specific for Na+-D-glucose cotransport.
1999,
Pubmed
,
Xenbase
Ruegg,
Bulk isolation of renal PCT and PST. I. Glucose-dependent metabolic differences.
1990,
Pubmed
Ruegg,
Differential patterns of injury to the proximal tubule of renal cortical slices following in vitro exposure to mercuric chloride, potassium dichromate, or hypoxic conditions.
1987,
Pubmed
Røigaard-Petersen,
Characteristics of D-galactose transport systems by luminal membrane vesicles from rabbit kidney.
1986,
Pubmed
Sakhrani,
Transport and metabolism of glucose by renal proximal tubular cells in primary culture.
1984,
Pubmed
Segal,
Developmental and other characteristics of -methyl-D-glucoside transport by rat kidney cortex slices.
1973,
Pubmed
Taub,
The development of serum-free hormone-supplemented media for primary kidney cultures and their use in examining renal functions.
1981,
Pubmed
Turk,
Glucose/galactose malabsorption caused by a defect in the Na+/glucose cotransporter.
1991,
Pubmed
,
Xenbase
Turner,
Further studies of proximal tubular brush border membrane D-glucose transport heterogeneity.
1982,
Pubmed
Turner,
Heterogeneity of sodium-dependent D-glucose transport sites along the proximal tubule: evidence from vesicle studies.
1982,
Pubmed
Turner,
Stoichiometric studies of the renal outer cortical brush border membrane D-glucose transporter.
1982,
Pubmed
Veyhl,
Cloning of a membrane-associated protein which modifies activity and properties of the Na(+)-D-glucose cotransporter.
1993,
Pubmed
,
Xenbase
Wells,
Localization of the Na+/glucose cotransporter gene SGLT2 to human chromosome 16 close to the centromere.
1993,
Pubmed
Yoneyama,
Increased trehalase expression after glucose limitation of LLC-PK1 clones.
1988,
Pubmed
You,
Molecular characteristics of Na(+)-coupled glucose transporters in adult and embryonic rat kidney.
1995,
Pubmed
,
Xenbase
