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Nucleic Acids Res
2002 Oct 01;3019:4176-85. doi: 10.1093/nar/gkf532.
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Human Mcm proteins at a replication origin during the G1 to S phase transition.
Schaarschmidt D
,
Ladenburger EM
,
Keller C
,
Knippers R
.
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Previous work with yeast cells and with Xenopus egg extracts had shown that eukaryotic pre-replication complexes assemble on chromatin in a step-wise manner whereby specific loading factors promote the recruitment of essential Mcm proteins at pre-bound origin recognition complexes (ORC with proteins Orc1p-Orc6p). While the order of assembly--Mcm binding follows ORC binding--seems to be conserved in cycling mammalian cells in culture, it has not been determined whether mammalian Mcm proteins associate with ORC-bearing chromatin sites. We have used a chromatin immunoprecipitation approach to investigate the site of Mcm binding in a genomic region that has previously been shown to contain an ORC-binding site and an origin of replication. Using chromatin from HeLa cells in G1 phase, antibodies against Orc2p as well as antibodies against Mcm proteins specifically immunoprecipitate chromatin enriched for a DNA region that includes a replication origin. However, with chromatin from cells in S phase, only Orc2p-specific antibodies immunoprecipitate the origin-containing DNA region while Mcm-specific antibodies immunoprecipitate chromatin with DNA from all parts of the genomic region investigated. Thus, human Mcm proteins first assemble at or adjacent to bound ORC and move to other sites during genome replication.
Alexandrow,
A potential role for mini-chromosome maintenance (MCM) proteins in initiation at the dihydrofolate reductase replication origin.
2002, Pubmed
Alexandrow,
A potential role for mini-chromosome maintenance (MCM) proteins in initiation at the dihydrofolate reductase replication origin.
2002,
Pubmed
Aparicio,
Components and dynamics of DNA replication complexes in S. cerevisiae: redistribution of MCM proteins and Cdc45p during S phase.
1997,
Pubmed
Chong,
Purification of an MCM-containing complex as a component of the DNA replication licensing system.
1995,
Pubmed
,
Xenbase
Chong,
A double-hexamer archaeal minichromosome maintenance protein is an ATP-dependent DNA helicase.
2000,
Pubmed
Connelly,
The promoters for human DNA-PKcs (PRKDC) and MCM4: divergently transcribed genes located at chromosome 8 band q11.
1998,
Pubmed
DULBECCO,
Plaque formation and isolation of pure lines with poliomyelitis viruses.
1954,
Pubmed
DaFonseca,
Identification of two residues in MCM5 critical for the assembly of MCM complexes and Stat1-mediated transcription activation in response to IFN-gamma.
2001,
Pubmed
Dalton,
Characterization of Cdc47p-minichromosome maintenance complexes in Saccharomyces cerevisiae: identification of Cdc45p as a subunit.
1997,
Pubmed
Devault,
Identification of Tah11/Sid2 as the ortholog of the replication licensing factor Cdt1 in Saccharomyces cerevisiae.
2002,
Pubmed
,
Xenbase
Dimitrova,
Mammalian nuclei become licensed for DNA replication during late telophase.
2002,
Pubmed
,
Xenbase
Dimitrova,
Regulation of mammalian replication origin usage in Xenopus egg extract.
1998,
Pubmed
,
Xenbase
Donovan,
Cdc6p-dependent loading of Mcm proteins onto pre-replicative chromatin in budding yeast.
1997,
Pubmed
,
Xenbase
Fujita,
Nuclear organization of DNA replication initiation proteins in mammalian cells.
2002,
Pubmed
Fujita,
In vivo interaction of human MCM heterohexameric complexes with chromatin. Possible involvement of ATP.
1997,
Pubmed
Gómez,
Different phenotypes in vivo are associated with ATPase motif mutations in Schizosaccharomyces pombe minichromosome maintenance proteins.
2002,
Pubmed
Göhring,
The scaffold/matrix attachment region binding protein hnRNP-U (SAF-A) is directly bound to chromosomal DNA in vivo: a chemical cross-linking study.
1997,
Pubmed
Holthoff,
Human protein MCM6 on HeLa cell chromatin.
1998,
Pubmed
Ishimi,
Biochemical function of mouse minichromosome maintenance 2 protein.
1998,
Pubmed
Ishimi,
A DNA helicase activity is associated with an MCM4, -6, and -7 protein complex.
1997,
Pubmed
Kearsey,
MCM proteins: evolution, properties, and role in DNA replication.
1998,
Pubmed
Keller,
The origin recognition complex marks a replication origin in the human TOP1 gene promoter.
2002,
Pubmed
Kelman,
The single minichromosome maintenance protein of Methanobacterium thermoautotrophicum DeltaH contains DNA helicase activity.
1999,
Pubmed
Kimura,
DNA polymerase alpha associated protein P1, a murine homolog of yeast MCM3, changes its intranuclear distribution during the DNA synthetic period.
1994,
Pubmed
Koonin,
A common set of conserved motifs in a vast variety of putative nucleic acid-dependent ATPases including MCM proteins involved in the initiation of eukaryotic DNA replication.
1993,
Pubmed
Kreitz,
The human origin recognition complex protein 1 dissociates from chromatin during S phase in HeLa cells.
2001,
Pubmed
,
Xenbase
Krude,
Human replication proteins hCdc21, hCdc46 and P1Mcm3 bind chromatin uniformly before S-phase and are displaced locally during DNA replication.
1996,
Pubmed
,
Xenbase
Kubota,
Licensing of DNA replication by a multi-protein complex of MCM/P1 proteins in Xenopus eggs.
1997,
Pubmed
,
Xenbase
Kubota,
Identification of the yeast MCM3-related protein as a component of Xenopus DNA replication licensing factor.
1995,
Pubmed
,
Xenbase
Labib,
MCM2-7 proteins are essential components of prereplicative complexes that accumulate cooperatively in the nucleus during G1-phase and are required to establish, but not maintain, the S-phase checkpoint.
2001,
Pubmed
Labib,
Uninterrupted MCM2-7 function required for DNA replication fork progression.
2000,
Pubmed
Ladenburger,
MCM4 and PRKDC, human genes encoding proteins MCM4 and DNA-PKcs, are close neighbours located on chromosome 8q12-->q13.
1997,
Pubmed
Ladenburger,
Identification of a binding region for human origin recognition complex proteins 1 and 2 that coincides with an origin of DNA replication.
2002,
Pubmed
Lee,
Processive DNA helicase activity of the minichromosome maintenance proteins 4, 6, and 7 complex requires forked DNA structures.
2001,
Pubmed
Lee,
Isolation and characterization of various complexes of the minichromosome maintenance proteins of Schizosaccharomyces pombe.
2000,
Pubmed
Lei,
Initiating DNA synthesis: from recruiting to activating the MCM complex.
2001,
Pubmed
Lei,
Physical interactions among Mcm proteins and effects of Mcm dosage on DNA replication in Saccharomyces cerevisiae.
1996,
Pubmed
Li,
Selective activation of pre-replication complexes in vitro at specific sites in mammalian nuclei.
2000,
Pubmed
,
Xenbase
Liang,
Persistent initiation of DNA replication and chromatin-bound MCM proteins during the cell cycle in cdc6 mutants.
1997,
Pubmed
Lygerou,
Cell cycle. License withheld--geminin blocks DNA replication.
2000,
Pubmed
,
Xenbase
Madine,
Geminin bans replication licence.
2001,
Pubmed
,
Xenbase
Méndez,
Human origin recognition complex large subunit is degraded by ubiquitin-mediated proteolysis after initiation of DNA replication.
2002,
Pubmed
Méndez,
Chromatin association of human origin recognition complex, cdc6, and minichromosome maintenance proteins during the cell cycle: assembly of prereplication complexes in late mitosis.
2000,
Pubmed
,
Xenbase
Neuwald,
AAA+: A class of chaperone-like ATPases associated with the assembly, operation, and disassembly of protein complexes.
1999,
Pubmed
Pasion,
Deconstructing a conserved protein family: the role of MCM proteins in eukaryotic DNA replication.
2001,
Pubmed
Richter,
Mobilization of chromatin-bound Mcm proteins by micrococcal nuclease.
1998,
Pubmed
Ritzi,
Human minichromosome maintenance proteins and human origin recognition complex 2 protein on chromatin.
1998,
Pubmed
Romanowski,
XMCM7, a novel member of the Xenopus MCM family, interacts with XMCM3 and colocalizes with it throughout replication.
1996,
Pubmed
,
Xenbase
Romanowski,
The Xenopus origin recognition complex is essential for DNA replication and MCM binding to chromatin.
1996,
Pubmed
,
Xenbase
Rowles,
Interaction between the origin recognition complex and the replication licensing system in Xenopus.
1996,
Pubmed
,
Xenbase
Santocanale,
Activation of dormant origins of DNA replication in budding yeast.
1999,
Pubmed
Schwacha,
Interactions between two catalytically distinct MCM subgroups are essential for coordinated ATP hydrolysis and DNA replication.
2001,
Pubmed
Seki,
Stepwise assembly of initiation proteins at budding yeast replication origins in vitro.
2000,
Pubmed
Shechter,
The intrinsic DNA helicase activity of Methanobacterium thermoautotrophicum delta H minichromosome maintenance protein.
2000,
Pubmed
Sterner,
Negative regulation of DNA replication by the retinoblastoma protein is mediated by its association with MCM7.
1998,
Pubmed
,
Xenbase
Su,
Drosophila MCM protein complexes.
1996,
Pubmed
Tada,
Repression of origin assembly in metaphase depends on inhibition of RLF-B/Cdt1 by geminin.
2001,
Pubmed
,
Xenbase
Tanaka,
Loading of an Mcm protein onto DNA replication origins is regulated by Cdc6p and CDKs.
1997,
Pubmed
Thömmes,
The DNA replication licensing system.
1997,
Pubmed
,
Xenbase
Todorov,
BM28, a human member of the MCM2-3-5 family, is displaced from chromatin during DNA replication.
1995,
Pubmed
Treuner,
Phosphorylation of replication protein A middle subunit (RPA32) leads to a disassembly of the RPA heterotrimer.
1999,
Pubmed
Tye,
MCM proteins in DNA replication.
1999,
Pubmed
Wohlschlegel,
Inhibition of eukaryotic DNA replication by geminin binding to Cdt1.
2000,
Pubmed
,
Xenbase
Wold,
Replication protein A: a heterotrimeric, single-stranded DNA-binding protein required for eukaryotic DNA metabolism.
1997,
Pubmed
Wyrick,
Genome-wide distribution of ORC and MCM proteins in S. cerevisiae: high-resolution mapping of replication origins.
2001,
Pubmed
Yan,
Mcm2 and Mcm3, two proteins important for ARS activity, are related in structure and function.
1991,
Pubmed
Yan,
Cell cycle-regulated nuclear localization of MCM2 and MCM3, which are required for the initiation of DNA synthesis at chromosomal replication origins in yeast.
1993,
Pubmed
Yankulov,
MCM proteins are associated with RNA polymerase II holoenzyme.
1999,
Pubmed
,
Xenbase
You,
Biochemical analysis of the intrinsic Mcm4-Mcm6-mcm7 DNA helicase activity.
1999,
Pubmed
Zhang,
Ser727-dependent recruitment of MCM5 by Stat1alpha in IFN-gamma-induced transcriptional activation.
1998,
Pubmed