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Mol Biol Cell
2002 Oct 01;1310:3696-705. doi: 10.1091/mbc.e02-03-0127.
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An endogenous RNA transcript antisense to CNG(alpha)1 cation channel mRNA.
Cheng CH
,
Yew DT
,
Kwan HY
,
Zhou Q
,
Huang Y
,
Liu Y
,
Chan WY
,
Yao X
.
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CNG channels are cyclic nucleotide-gated Ca(2+)-permeable channels that are suggested to be involved in the activity-dependent alterations of synaptic strength that are thought to underlie information storage in the CNS. In this study, we isolated an endogenous RNA transcript antisense to CNG(alpha)1 mRNA. This transcript was capable of down-regulating the expression of sense CNG(alpha)1 in the Xenopus oocyte expression system. RT-PCR, Northern blot, and in situ hybridization analyses showed that the transcript was coexpressed with CNG(alpha)1 mRNA in many regions of human brain, notably in those regions that were involved in long-term potentiation and long-term depression, such as hippocampal CA1 and CA3, dentate gyrus, and cerebellar Purkinje layer. Comparison of expression patterns between adult and fetal cerebral cortex revealed that there were concurrent developmental changes in the expression levels of anti-CNG1 and CNG(alpha)1. Treatment of human glioma cell T98 with thyroid hormone T(3) caused a significant increase in anti-CNG1 expression and a parallel decrease in sense CNG(alpha)1 expression. These data suggest that the suppression of CNG(alpha)1 expression by anti-CNG1 may play an important role in neuronal functions, especially in synaptic plasticity and cortical development. Endogenous antisense RNA-mediated regulation may represent a new mechanism through which the activity of ion channels can be regulated in the human CNS.
Ahmad,
Retinal ganglion cells express a cGMP-gated cation conductance activatable by nitric oxide donors.
1994, Pubmed
Ahmad,
Retinal ganglion cells express a cGMP-gated cation conductance activatable by nitric oxide donors.
1994,
Pubmed
Arancio,
Activity-dependent long-term enhancement of transmitter release by presynaptic 3',5'-cyclic GMP in cultured hippocampal neurons.
1995,
Pubmed
Bradley,
Functional expression of the heteromeric "olfactory" cyclic nucleotide-gated channel in the hippocampus: a potential effector of synaptic plasticity in brain neurons.
1997,
Pubmed
Chomczynski,
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
1987,
Pubmed
Coburn,
A putative cyclic nucleotide-gated channel is required for sensory development and function in C. elegans.
1996,
Pubmed
Crary,
Mechanism of inhibition of cyclic nucleotide-gated ion channels by diacylglycerol.
2000,
Pubmed
,
Xenbase
Dhallan,
Human rod photoreceptor cGMP-gated channel: amino acid sequence, gene structure, and functional expression.
1992,
Pubmed
Dolnick,
Naturally occurring antisense RNA.
1997,
Pubmed
Dussault,
Thyroid hormones and brain development.
1987,
Pubmed
Dzeja,
Ca2+ permeation in cyclic nucleotide-gated channels.
1999,
Pubmed
Finn,
Cyclic nucleotide-gated ion channels: an extended family with diverse functions.
1996,
Pubmed
Hildebrandt,
Differential antisense transcription from the Dictyostelium EB4 gene locus: implications on antisense-mediated regulation of mRNA stability.
1992,
Pubmed
Hsu,
Modulation of the cGMP-gated channel of rod photoreceptor cells by calmodulin.
1993,
Pubmed
Kaupp,
Family of cyclic nucleotide gated ion channels.
1995,
Pubmed
Kimelman,
An antisense mRNA directs the covalent modification of the transcript encoding fibroblast growth factor in Xenopus oocytes.
1989,
Pubmed
,
Xenbase
Kingston,
Rat hippocampal neurons express genes for both rod retinal and olfactory cyclic nucleotide-gated channels: novel targets for cAMP/cGMP function.
1996,
Pubmed
Knee,
Basic fibroblast growth factor sense (FGF) and antisense (gfg) RNA transcripts are expressed in unfertilized human oocytes and in differentiated adult tissues.
1994,
Pubmed
Laabi,
The BCMA gene, preferentially expressed during B lymphoid maturation, is bidirectionally transcribed.
1994,
Pubmed
Leinders-Zufall,
Regulation of cyclic nucleotide-gated channels and membrane excitability in olfactory receptor cells by carbon monoxide.
1995,
Pubmed
Molokanova,
Modulation of rod photoreceptor cyclic nucleotide-gated channels by tyrosine phosphorylation.
1997,
Pubmed
,
Xenbase
Nevins,
The pathway of eukaryotic mRNA formation.
1983,
Pubmed
Oppenheimer,
Molecular basis of thyroid hormone-dependent brain development.
1997,
Pubmed
Petersen,
The regulation of capacitative calcium entry by calcium and protein kinase C in Xenopus oocytes.
1994,
Pubmed
,
Xenbase
Rieke,
A cGMP-gated current can control exocytosis at cone synapses.
1994,
Pubmed
Samanta Roy,
Temporal and spatial pattern of expression of cyclic nucleotide-gated channels in developing rat visual cortex.
1999,
Pubmed
Savchenko,
Cyclic-nucleotide-gated channels mediate synaptic feedback by nitric oxide.
,
Pubmed
Shiells,
Glutamate receptors of rod bipolar cells are linked to a cyclic GMP cascade via a G-protein.
1990,
Pubmed
Simons,
Naturally occurring antisense RNA control--a brief review.
1988,
Pubmed
Strijbos,
Molecular characterization and in situ localization of a full-length cyclic nucleotide-gated channel in rat brain.
1999,
Pubmed
Thrash-Bingham,
aHIF: a natural antisense transcript overexpressed in human renal cancer and during hypoxia.
1999,
Pubmed
Vanhée-Brossollet,
Do natural antisense transcripts make sense in eukaryotes?
1998,
Pubmed
Wutz,
Imprinted expression of the Igf2r gene depends on an intronic CpG island.
1997,
Pubmed
Yao,
Primary structure and functional expression of a cGMP-gated potassium channel.
1995,
Pubmed
,
Xenbase
Yew,
Nitric oxide synthase neurons in different areas of normal aged and Alzheimer's brains.
1999,
Pubmed
Zagotta,
Structure and function of cyclic nucleotide-gated channels.
1996,
Pubmed
Zufall,
Cyclic nucleotide gated channels as regulators of CNS development and plasticity.
1997,
Pubmed