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XB-ART-6148
Appl Environ Microbiol 2002 Dec 01;6812:6403-4. doi: 10.1128/AEM.68.12.6403-6404.2002.
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The environmental pathogen Mycobacterium ulcerans grows in amphibian cells at low temperatures.

Drancourt M , Jarlier V , Raoult D .


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Mycobacterium ulcerans, the etiological agent of Buruli ulcers, is an environmental pathogen. We cultivated it in an amphibian (XTC-2) cell line that grows at 28 degrees C. By counting of Ziehl-Neelsen-stained mycobacteria and by quantitative PCR analysis, we found that M. ulcerans multiplies rapidly in association with XTC-2 cells. Transmission electron microscopy demonstrated the presence of intracellular M. ulcerans microorganisms. These data suggest an intracellular environmental niche, and we propose use of XTC-2 cells for isolation of M. ulcerans from environmental sources.

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References [+] :
Chemlal, Evaluation of PCR-restriction profile analysis and IS2404 restriction fragment length polymorphism and amplified fragment length polymorphism fingerprinting for identification and typing of Mycobacterium ulcerans and M. marinum. 2001, Pubmed