Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
J Membr Biol
2023 Dec 01;2564-6:317-330. doi: 10.1007/s00232-023-00286-w.
Show Gene links
Show Anatomy links
Peptide Flexibility and the Hydrophobic Moment are Determinants to Evaluate the Clinical Potential of Magainins.
Balleza D
.
???displayArticle.abstract???
Using a flexibility prediction algorithm and in silico structural modeling, we have calculated the intrinsic flexibility of several magainin derivatives. In the case of magainin-2 (Mag-2) and magainin H2 (MAG-H2) we have found that MAG-2 is more flexible than its hydrophobic analog, Mag-H2. This affects the degree of bending of both peptides, with a kink around two central residues (R10, R11), whereas, in Mag-H2, W10 stiffens the peptide. Moreover, this increases the hydrophobic moment of Mag-H2, which could explain its propensity to form pores in POPC model membranes, which exhibit near-to-zero spontaneous curvatures. Likewise, the protective effect described in DOPC membranes for this peptide regarding its facilitation in pore formation would be related to the propensity of this lipid to form membranes with negative spontaneous curvature. The flexibility of another magainin analog (MSI-78) is even greater than that of Mag-2. This facilitates the peptide to present a kind of hinge around the central F12 as well as a C-terminal end prone to be disordered. Such characteristics are key to understanding the broad-spectrum antimicrobial actions exhibited by this peptide. These data reinforce the hypothesis on the determinant role of spontaneous membrane curvature, intrinsic peptide flexibility, and specific hydrophobic moment in assessing the bioactivity of membrane-active antimicrobial peptides.
Ageitos,
Antimicrobial peptides (AMPs): Ancient compounds that represent novel weapons in the fight against bacteria.
2017, Pubmed
Ageitos,
Antimicrobial peptides (AMPs): Ancient compounds that represent novel weapons in the fight against bacteria.
2017,
Pubmed
Aisenbrey,
The Mechanisms of Action of Cationic Antimicrobial Peptides Refined by Novel Concepts from Biophysical Investigations.
2019,
Pubmed
Balleza,
Role of Lipid Composition, Physicochemical Interactions, and Membrane Mechanics in the Molecular Actions of Microbial Cyclic Lipopeptides.
2019,
Pubmed
Balleza,
Model lipid systems and their use to evaluate the phase state of biomembranes, their mechanical properties and the effect of non-conventional antibiotics: the case of daptomycin.
2020,
Pubmed
Bechinger,
The structure, dynamics and orientation of antimicrobial peptides in membranes by multidimensional solid-state NMR spectroscopy.
1999,
Pubmed
,
Xenbase
Bechinger,
Revealing the Mechanisms of Synergistic Action of Two Magainin Antimicrobial Peptides.
2020,
Pubmed
Bennett,
The importance of membrane defects-lessons from simulations.
2014,
Pubmed
Brogden,
Antimicrobial peptides: pore formers or metabolic inhibitors in bacteria?
2005,
Pubmed
Carugo,
Correlation between side chain mobility and conformation in protein structures.
1997,
Pubmed
Cheng,
The Interactions between the Antimicrobial Peptide P-113 and Living Candida albicans Cells Shed Light on Mechanisms of Antifungal Activity and Resistance.
2020,
Pubmed
Denardi,
In vitro activity of the antimicrobial peptides h-Lf1-11, MSI-78, LL-37, fengycin 2B, and magainin-2 against clinically important bacteria.
2022,
Pubmed
Dickey,
Examining the contributions of lipid shape and headgroup charge on bilayer behavior.
2008,
Pubmed
Dixon,
Antimicrobial peptide inhibition of Porphyromonas gingivalis 381-induced hemagglutination is improved with a synthetic decapeptide.
2009,
Pubmed
Eicher,
Intrinsic Curvature-Mediated Transbilayer Coupling in Asymmetric Lipid Vesicles.
2018,
Pubmed
Eisenberg,
The helical hydrophobic moment: a measure of the amphiphilicity of a helix.
1982,
Pubmed
Epand,
Lipid domains in bacterial membranes and the action of antimicrobial agents.
2009,
Pubmed
Fuchs,
In vitro antimicrobial activity of MSI-78, a magainin analog.
1998,
Pubmed
Gautier,
HELIQUEST: a web server to screen sequences with specific alpha-helical properties.
2008,
Pubmed
Ge,
In vitro antibacterial properties of pexiganan, an analog of magainin.
1999,
Pubmed
Gesell,
Two-dimensional 1H NMR experiments show that the 23-residue magainin antibiotic peptide is an alpha-helix in dodecylphosphocholine micelles, sodium dodecylsulfate micelles, and trifluoroethanol/water solution.
1997,
Pubmed
,
Xenbase
Gottler,
Structure, membrane orientation, mechanism, and function of pexiganan--a highly potent antimicrobial peptide designed from magainin.
2009,
Pubmed
,
Xenbase
Hale,
Alternative mechanisms of action of cationic antimicrobial peptides on bacteria.
2007,
Pubmed
Hallock,
MSI-78, an analogue of the magainin antimicrobial peptides, disrupts lipid bilayer structure via positive curvature strain.
2003,
Pubmed
,
Xenbase
Hills,
Model parameters for simulation of physiological lipids.
2016,
Pubmed
Hossain,
Single-Cell Analysis of the Antimicrobial and Bactericidal Activities of the Antimicrobial Peptide Magainin 2.
2022,
Pubmed
Juhl,
Antimicrobial peptides: mechanism of action and lipid-mediated synergistic interactions within membranes.
2021,
Pubmed
Jumper,
Highly accurate protein structure prediction with AlphaFold.
2021,
Pubmed
Kabelka,
Advances in Molecular Understanding of α-Helical Membrane-Active Peptides.
2021,
Pubmed
Kandasamy,
Binding and insertion of alpha-helical anti-microbial peptides in POPC bilayers studied by molecular dynamics simulations.
2004,
Pubmed
,
Xenbase
Kaplan,
Selective membrane disruption: mode of action of C16G2, a specifically targeted antimicrobial peptide.
2011,
Pubmed
Kucerka,
Lipid bilayer structure determined by the simultaneous analysis of neutron and X-ray scattering data.
2008,
Pubmed
Kučerka,
Fluid phase lipid areas and bilayer thicknesses of commonly used phosphatidylcholines as a function of temperature.
2011,
Pubmed
Lamiable,
PEP-FOLD3: faster de novo structure prediction for linear peptides in solution and in complex.
2016,
Pubmed
Lee,
Lipid composition-dependent membrane fragmentation and pore-forming mechanisms of membrane disruption by pexiganan (MSI-78).
2013,
Pubmed
Lee,
Antimicrobial Peptide Structure and Mechanism of Action: A Focus on the Role of Membrane Structure.
2016,
Pubmed
Leontiadou,
Antimicrobial peptides in action.
2006,
Pubmed
Magana,
The value of antimicrobial peptides in the age of resistance.
2020,
Pubmed
Mahlapuu,
Antimicrobial peptides as therapeutic agents: opportunities and challenges.
2020,
Pubmed
Marquette,
Biophysical Investigations Elucidating the Mechanisms of Action of Antimicrobial Peptides and Their Synergism.
2018,
Pubmed
Marsh,
Intrinsic curvature in normal and inverted lipid structures and in membranes.
1996,
Pubmed
Marsh,
Lateral pressure profile, spontaneous curvature frustration, and the incorporation and conformation of proteins in membranes.
2007,
Pubmed
Marín-Medina,
Effects of the peptide Magainin H2 on Supported Lipid Bilayers studied by different biophysical techniques.
2018,
Pubmed
Matsuzaki,
Molecular basis for membrane selectivity of an antimicrobial peptide, magainin 2.
1995,
Pubmed
,
Xenbase
Matsuzaki,
Interactions of an antimicrobial peptide, magainin 2, with outer and inner membranes of Gram-negative bacteria.
1997,
Pubmed
,
Xenbase
Matsuzaki,
Relationship of membrane curvature to the formation of pores by magainin 2.
1998,
Pubmed
,
Xenbase
McHenry,
Does cholesterol suppress the antimicrobial peptide induced disruption of lipid raft containing membranes?
2012,
Pubmed
Melo,
Omiganan interaction with bacterial membranes and cell wall models. Assigning a biological role to saturation.
2007,
Pubmed
Mercer,
NP213 (Novexatin®): A unique therapy candidate for onychomycosis with a differentiated safety and efficacy profile.
2020,
Pubmed
Mescola,
Magainin-H2 effects on the permeabilization and mechanical properties of giant unilamellar vesicles.
2019,
Pubmed
Mosgaard,
Lipid ion channels and the role of proteins.
2013,
Pubmed
Nguyen,
The expanding scope of antimicrobial peptide structures and their modes of action.
2011,
Pubmed
Parreira,
Surface Grafted MSI-78A Antimicrobial Peptide has High Potential for Gastric Infection Management.
2019,
Pubmed
Paterson,
Lipid topology and electrostatic interactions underpin lytic activity of linear cationic antimicrobial peptides in membranes.
2017,
Pubmed
Pius,
²H solid-state nuclear magnetic resonance investigation of whole Escherichia coli interacting with antimicrobial peptide MSI-78.
2012,
Pubmed
Ramamoorthy,
Solid-state NMR investigation of the membrane-disrupting mechanism of antimicrobial peptides MSI-78 and MSI-594 derived from magainin 2 and melittin.
2006,
Pubmed
,
Xenbase
Ramos-Martín,
Biomembrane lipids: When physics and chemistry join to shape biological activity.
2022,
Pubmed
Reißer,
3D hydrophobic moment vectors as a tool to characterize the surface polarity of amphiphilic peptides.
2014,
Pubmed
Ryu,
Rediscovery of antimicrobial peptides as therapeutic agents.
2021,
Pubmed
Saravolatz,
In vitro activities of LTX-109, a synthetic antimicrobial peptide, against methicillin-resistant, vancomycin-intermediate, vancomycin-resistant, daptomycin-nonsusceptible, and linezolid-nonsusceptible Staphylococcus aureus.
2012,
Pubmed
Seelig,
Thermodynamics of lipid-peptide interactions.
2004,
Pubmed
Smith,
Improved amino acid flexibility parameters.
2003,
Pubmed
Strahl,
Bacterial Membranes: Structure, Domains, and Function.
2017,
Pubmed
Strandberg,
Lipid shape is a key factor for membrane interactions of amphipathic helical peptides.
2012,
Pubmed
Sun,
Utility of B-Factors in Protein Science: Interpreting Rigidity, Flexibility, and Internal Motion and Engineering Thermostability.
2019,
Pubmed
Syvitski,
Structural characterization of the antimicrobial peptide pleurocidin from winter flounder.
2005,
Pubmed
Szule,
The effects of acyl chain length and saturation of diacylglycerols and phosphatidylcholines on membrane monolayer curvature.
2002,
Pubmed
Tachi,
Position-dependent hydrophobicity of the antimicrobial magainin peptide affects the mode of peptide-lipid interactions and selective toxicity.
2002,
Pubmed
,
Xenbase
Tamba,
Single giant unilamellar vesicle method reveals effect of antimicrobial peptide magainin 2 on membrane permeability.
2005,
Pubmed
,
Xenbase
Vanni,
A sub-nanometre view of how membrane curvature and composition modulate lipid packing and protein recruitment.
2014,
Pubmed
Yasir,
Comparative mode of action of the antimicrobial peptide melimine and its derivative Mel4 against Pseudomonas aeruginosa.
2019,
Pubmed
Zasloff,
Magainins, a class of antimicrobial peptides from Xenopus skin: isolation, characterization of two active forms, and partial cDNA sequence of a precursor.
1987,
Pubmed
,
Xenbase
Zerweck,
Molecular mechanism of synergy between the antimicrobial peptides PGLa and magainin 2.
2017,
Pubmed
Zhan,
Influence of the membrane dipole potential on peptide binding to lipid bilayers.
2012,
Pubmed
