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Dev Growth Differ
1996 Oct 01;385:527-535. doi: 10.1046/j.1440-169X.1996.t01-4-00009.x.
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Spatio-temporal distribution of the protein of Xenopus vasa homologue (Xenopus vasa-like gene 1, XVLG1) in embryos.
Ikenishi K
,
Tanaka TS
,
Komiya T
.
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In order to know when the protein of Xenopus vasa homolog (Xenopus vasa-like gene 1, XVLG1) first appears in germ line cells and whether the protein is also present in somatic cells as is vasa protein in Drosophila, the spatio-temporal distribution of the protein in Xenopus embryos was carefully investigated by fluorescent microscopy. Part of the observation was performed by whole-mount immunocytochemistry and immunoblotting. A distinct fluorescence of XVLG1 protein was first recognized in a juxta-nuclear location of germ line cells or presumptive primordial germ cells (pPGC) at stage 12 (late gastrula) and remained associated with the pPGC or primordial germ cells (PGC) throughout the following stages until stage 46 (feeding tadpole). In contrast, weak fluorescence was seen in the animal hemisphere rather than in the vegetal hemisphere of cleaving embryos and in the perinuclear region of somatic cells at stages 10-42 (early gastrula to young tadpole), respectively. Nearly the same pattern as revealed by fluorescence was seen by whole-mount immunocytochemistry, except that a small amount of XVLG1 protein seemed to be present in the germ plasm and pPGC of embryos earlier than stage 12. The presence of the protein in the somatic cells and the PGC was also shown by immunoblotting.
BLACKLER,
Contribution to the study of germ-cells in the anura.
1958, Pubmed
BLACKLER,
Contribution to the study of germ-cells in the anura.
1958,
Pubmed
Balinsky,
Changes in the ultrastructure of amphibian eggs following fertilization.
1966,
Pubmed
Bardsley,
Distribution of tudor protein in the Drosophila embryo suggests separation of functions based on site of localization.
1993,
Pubmed
Boswell,
tudor, a gene required for assembly of the germ plasm in Drosophila melanogaster.
1985,
Pubmed
Boswell,
Mutations in a newly identified Drosophila melanogaster gene, mago nashi, disrupt germ cell formation and result in the formation of mirror-image symmetrical double abdomen embryos.
1991,
Pubmed
Dumont,
Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals.
1972,
Pubmed
,
Xenbase
Eddy,
Germ plasm and the differentiation of the germ cell line.
1975,
Pubmed
,
Xenbase
Eppig,
Oogenesis in Xenopus laevis (Daudin). II. The induction and subcellular localization of tyrosinase activity in developing oocytes.
1974,
Pubmed
,
Xenbase
Gururajan,
An3 mRNA encodes an RNA helicase that colocalizes with nucleoli in Xenopus oocytes in a stage-specific manner.
1994,
Pubmed
,
Xenbase
Hay,
A protein component of Drosophila polar granules is encoded by vasa and has extensive sequence similarity to ATP-dependent helicases.
1988,
Pubmed
Hay,
Identification of a component of Drosophila polar granules.
1988,
Pubmed
Heasman,
Primordial germ cells of Xenopus embryos: the role of fibronectin in their adhesion during migration.
1981,
Pubmed
,
Xenbase
Ijiri,
Mitotic activity of germ cells during normal development of Xenopus laevis tadpoles.
1975,
Pubmed
,
Xenbase
Ikenishi,
Ultraviolet effects on presumptive primordial germ cells (pPGCs) in Xenopus laevis after the cleavage stage.
1979,
Pubmed
,
Xenbase
Ikenishi,
A possible maternal-effect mutant of Xenopus laevis I. Cytological and biochemical analyses of the unfertilized eggs and embryos.
1988,
Pubmed
,
Xenbase
Itoh,
The expression of epidermal antigens in Xenopus laevis.
1988,
Pubmed
,
Xenbase
Kamimura,
Observations on the migration and proliferation of gonocytes in Xenopus laevis.
1976,
Pubmed
,
Xenbase
Kobayashi,
Presence of mitochondrial large ribosomal RNA outside mitochondria in germ plasm of Drosophila melanogaster.
1993,
Pubmed
Kobayashi,
Restoration of pole-cell-forming ability to u.v.-irradiated Drosophila embryos by injection of mitochondrial lrRNA.
1989,
Pubmed
Kobayashi,
Localization of mitochondrial large rRNA in germinal granules and the consequent segregation of germ line.
1994,
Pubmed
,
Xenbase
Komiya,
Isolation and characterization of a novel gene of the DEAD box protein family which is specifically expressed in germ cells of Xenopus laevis.
1994,
Pubmed
,
Xenbase
Kotani,
Cortical granules remaining after fertilization in Xenopus laevis.
1973,
Pubmed
,
Xenbase
Lasko,
The product of the Drosophila gene vasa is very similar to eukaryotic initiation factor-4A.
1988,
Pubmed
Lehmann,
Abdominal segmentation, pole cell formation, and embryonic polarity require the localized activity of oskar, a maternal gene in Drosophila.
1986,
Pubmed
Linder,
Birth of the D-E-A-D box.
1989,
Pubmed
Lipshitz,
Axis specification in the Drosophila embryo.
1991,
Pubmed
Mahowald,
Polar granules of Drosophila. II. Ultrastructural changes during early embryogenesis.
1968,
Pubmed
Mahowald,
Polar granules of Drosophila. 3. The continuity of polar granules during the life cycle of Drosophila.
1971,
Pubmed
Manseau,
cappuccino and spire: two unique maternal-effect loci required for both the anteroposterior and dorsoventral patterns of the Drosophila embryo.
1989,
Pubmed
Nakazato,
Monoclonal antibody production against a subcellular fraction of vegetal pole cytoplasm containing the germ plasm of Xenopus 2-cell eggs.
1989,
Pubmed
,
Xenbase
Wassarman,
RNA splicing. Alive with DEAD proteins.
1991,
Pubmed
