Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
The block to polyspermy in Xenopus laevis involves an interaction between a cortical granule lectin, released at fertilization, and a ligand located in the egg extracellular matrix. The egg extracellular matrix in X. laevis consists of a vitelline envelope and three distinct jelly layers, designated J1, J2 and J3. To localize cortical granule lectin ligand in the egg extracellular matrix, we used enzyme-linked lectin assays that showed that cortical granule lectin ligands were absent in J2, J3 and the vitelline envelope. Cortical granule lectin bound to a ligand(s) in J1 in a galactose-dependent fashion. In addition, we separated egg jelly macromolecules electrophoretically and, in conjunction with western blotting, have shown that J1 contains two major, high molecular weight ligands for cortical granule ligand. Finally, using confocal microscopy, we demonstrated that the ligand(s) for cortical granule lectin occupies a 20-30 μm thick band in a region of J1 just proximal to the vitelline envelope.
Bleil,
Sperm-egg interactions in the mouse: sequence of events and induction of the acrosome reaction by a zona pellucida glycoprotein.
1983, Pubmed
Bleil,
Sperm-egg interactions in the mouse: sequence of events and induction of the acrosome reaction by a zona pellucida glycoprotein.
1983,
Pubmed
Bleil,
Mammalian sperm-egg interaction: identification of a glycoprotein in mouse egg zonae pellucidae possessing receptor activity for sperm.
1980,
Pubmed
Bonnell,
Xenopus laevis egg jelly coats consist of small diffusible proteins bound to a complex system of structurally stable networks composed of high-molecular-weight glycoconjugates.
1996,
Pubmed
,
Xenbase
Focarelli,
The vitelline coat spikes: a new peculiar structure of Mytilus galloprovincialis eggs with a role in sperm-egg interaction.
1991,
Pubmed
Grey,
Formation and structure of the fertilization envelope in Xenopus laevis.
1974,
Pubmed
,
Xenbase
Hedrick,
Isolation of extracellular matrix structures from Xenopus laevis oocytes, eggs, and embryos.
1991,
Pubmed
,
Xenbase
Hedrick,
Structure and function of the extracellular matrix of anuran eggs.
1991,
Pubmed
,
Xenbase
Hedrick,
The incorporation and fate of [35-S]-sulfate in the jelly coat of Xenopus laevis eggs.
1974,
Pubmed
,
Xenbase
Mozingo,
Structural features of the abalone egg extracellular matrix and its role in gamete interaction during fertilization.
1995,
Pubmed
Nishihara,
Isolation and characterization of a lectin from the cortical granules of Xenopus laevis eggs.
1986,
Pubmed
,
Xenbase
Preobrazhensky,
Electrophoresis of neurochordins, a family of high molecular weight neural tissue glycoproteins, on horizontal submerged agarose gels in the presence of dodecyl sulfate.
1993,
Pubmed
Smith,
Measurement of protein using bicinchoninic acid.
1985,
Pubmed
Towbin,
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
1979,
Pubmed
Trimmer,
Activation of sea urchin gametes.
1986,
Pubmed
Wyrick,
Agglutination of jelly coat and cortical granule components and the block to polyspermy in the amphibian Xenopus laevis.
1974,
Pubmed
,
Xenbase