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Figure 1. Phylogenetic relationships, acoustics and external morphology of Nanohyla albopunctata sp. nov.
A: Type locality of Nanohyla albopunctata sp. nov. in Song Hinh Protected Forest, Phu Yen Province, Vietnam. B: Holotype of Nanohyla albopunctata sp. nov. (ZMMU A-7585) in dorsal view. C: Holotype of Nanohyla albopunctata sp. nov. (A-7585) in ventral view. D: Holotype of Nanohyla albopunctata sp. nov. (A-7585) in life. E: Sonogram of a male advertisement call of Nanohyla albopunctata sp. nov. F: Phylogenetic relationships of Nanohyla based on concatenated 12S−16S rRNA and BDNF gene sequences. For a full version of a tree see Supplementary Figure S3. Values at the nodes correspond to BIPP/UFB support values, respectively; filled circles represent strongly supported nodes with BPP and UFB support >0.95 and 95%, respectively; nodes lacking circles are not supported. For specimen and sequence data see Supplementary Table S1. Photos by N. A. Poyarkov.
Supplementary Figure S1 Holotype of Nanohyla albopunctata sp. nov. (ZMMU A-7587).
(A) Head in ventral view; (B) plantar view of left hand; (C) plantar view of left foot. IMT –
inner metatarsal tubercle, OMT – outer metatarsal tubercle. Photographs by P. V.
Yushchenko.
Supplementary Figure S2 Paratype males of Nanohyla albopunctata sp. nov. (ZMMU
A-7584, left; and ZMMU A-7586, right), in dorsal view in preservative. Photographs by P. V.
Yushchenko.
Supplementary Figure S3 Phylogenetic relationships of the
Microhyla-Nanohyla-Glyphoglossus assemblage. Bayesian inference tree derived from the
analysis of 3118 bp including long 12S—16S rRNA mtDNA and BDNF nuDNA gene
fragments. For voucher specimen information and GenBank accession numbers see
Supplementary Table S1. Colors denote genera of Microhyla-Nanohyla-Glyphoglossus
assemblage: Microhyla (orange), Nanohyla (red) and Glyphoglossus (blue). Numbers at tree
nodes correspond to the posterior probability (BI PP) and ultrafast bootstrap support (UFB)
values, respectively. Circles filled with black represent strongly supported nodes with BPP
and UFB support >0.95 and 95%, respectively; nodes lacking circles are not supported.
Kaloula baleata sequence was used as an outgroup. Photograph of the new species by
Nikolay A. Poyarkov.
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