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EMBO J
2003 Jan 15;222:246-51. doi: 10.1093/emboj/cdg026.
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Regulatable killing of eukaryotic cells by the prokaryotic proteins Kid and Kis.
de la Cueva-Méndez G
,
Mills AD
,
Clay-Farrace L
,
Díaz-Orejas R
,
Laskey RA
.
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Plasmid R1 inhibits growth of bacteria by synthesizing an inhibitor of cell proliferation, Kid, and a neutralizing antidote, Kis, which binds tightly to the toxin. Here we report that this toxin and antidote, which have evolved to function in bacteria, also function efficiently in a wide range of eukaryotes. Kid inhibits cell proliferation in yeast, Xenopus laevis and human cells, whilst Kis protects. Moreover, we show that Kid triggers apoptosis in human cells. These effects can be regulated in vivo by modulating the relative amounts of antidote and toxin using inducible eukaryotic promoters for independent transcriptional control of their genes. These findings allow highly regulatable, selective killing of eukaryotic cells, and could be applied to eliminate cancer cells or specific cell lineages in development.
Booth,
Glia maintain follower neuron survival during Drosophila CNS development.
2000,
Pubmed
Bravo,
Identification of components of a new stability system of plasmid R1, ParD, that is close to the origin of replication of this plasmid.
1987,
Pubmed
Bravo,
Killing of Escherichia coli cells modulated by components of the stability system ParD of plasmid R1.
1988,
Pubmed
Butler,
Nonradioactive in situ hybridization to xenopus tissue sections.
2001,
Pubmed
,
Xenbase
Cornelis,
Identification and characterization of a novel cell cycle-regulated internal ribosome entry site.
2000,
Pubmed
Evan,
A matter of life and cell death.
1998,
Pubmed
Fitzgerald,
Why toxins!
1996,
Pubmed
Giraldo,
Protein domains and conformational changes in the activation of RepA, a DNA replication initiator.
1998,
Pubmed
Hainaut,
p53 and human cancer: the first ten thousand mutations.
2000,
Pubmed
Hargreaves,
Structural and functional analysis of the kid toxin protein from E. coli plasmid R1.
2002,
Pubmed
Hensey,
A developmental timer that regulates apoptosis at the onset of gastrulation.
1997,
Pubmed
,
Xenbase
Jensen,
Programmed cell death in bacteria: proteic plasmid stabilization systems.
1995,
Pubmed
Lee,
Genetic ablation reveals that the roof plate is essential for dorsal interneuron specification.
2000,
Pubmed
Mascorro-Gallardo,
Construction of a CUP1 promoter-based vector to modulate gene expression in Saccharomyces cerevisiae.
1996,
Pubmed
McBratney,
Internal initiation of translation.
1993,
Pubmed
Mumberg,
Regulatable promoters of Saccharomyces cerevisiae: comparison of transcriptional activity and their use for heterologous expression.
1994,
Pubmed
Ruiz-Echevarría,
The kis and kid genes of the parD maintenance system of plasmid R1 form an operon that is autoregulated at the level of transcription by the co-ordinated action of the Kis and Kid proteins.
1991,
Pubmed
Ruiz-Echevarría,
Translational coupling and limited degradation of a polycistronic messenger modulate differential gene expression in the parD stability system of plasmid R1.
1995,
Pubmed
Shaham,
Death-defying yeast identify novel apoptosis genes.
1998,
Pubmed
Sikorski,
A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.
1989,
Pubmed
Tsuchimoto,
The stable maintenance system pem of plasmid R100: degradation of PemI protein may allow PemK protein to inhibit cell growth.
1992,
Pubmed
Yin,
Tetracycline-controlled gene expression system achieves high-level and quantitative control of gene expression.
1996,
Pubmed