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Figure 1. Time‐dependent accumulation of radiolabelled ranitidine, propranolol, and tetraethylammonium into Xenopus oocytes expressing zebrafish OCT2 (black squares) or sham injected controls (white circles). Values represent the average ± SEM of between seven and 15 oocytes.
Figure 2. The uptake rate of 200 µM radiolabelled tetraethylammonium into Xenopus oocytes expressing zebrafish OCT2 in the absence (control) and presence of 2 mM amantadine, cimetidine, propranolol, quinidine, and ranitidine. Values represent the average ± SEM of between seven and 15 oocytes. The columns with the same letter are not significantly different from each other (one‐way analysis of variance followed by post hoc Tukey's test, p < 0.05).
Figure 3. Dose‐dependent uptake rate of radiolabelled ranitidine and propranolol into Xenopus oocytes expressing zebrafish OCT2. Values represent the average ± SEM of uptake in seven to 15 OCT2 injected oocytes adjusted for the uptake in sham injected oocytes at each concentration. Michaelis–Menten parameters: ranitidine k
m = 246.1 µM, V
max = 44.6 pmol/(oocyte × min); propranolol k
m = 408.9 µM, V
max = 190 pmol/(oocyte × min).
Figure 4. The uptake rate of radiolabelled ranitidine, propranolol, and tetraethylammonium into Xenopus oocytes expressing zebrafish Oct2 (grey bars) or sham injected controls (white bars) at pH 6, 7, and 8. Values represent the average ± SEM of between seven and 15 oocytes. Asterisks indicate a significant difference between sham and Oct2 injected oocytes (Student's t‐test, p < 0.05) and A above the columns indicates a difference in the uptake rate in the Oct2 injected oocytes for propranolol at pH 7 and 8 compared with pH 6 (one‐way analysis of variance post hoc Tukey's test, p < 0.05).
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