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Identification and Analysis of Six Phosphorylation Sites Within the Xenopus laevis Linker Histone H1.0 C-Terminal Domain Indicate Distinct Effects on Nucleosome Structure.
Hao F
,
Mishra LN
,
Jaya P
,
Jones R
,
Hayes JJ
.
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As a key structural component of the chromatin of higher eukaryotes, linker histones (H1s) are involved in stabilizing the folding of extended nucleosome arrays into higher-order chromatin structures and function as a gene-specific regulator of transcription in vivo. The H1 C-terminal domain (CTD) is essential for high-affinity binding of linker histones to chromatin and stabilization of higher-order chromatin structure. Importantly, the H1 CTD is an intrinsically disordered domain that undergoes a drastic condensation upon binding to nucleosomes. Moreover, although phosphorylation is a prevalent post-translational modification within the H1 CTD, exactly where this modification is installed and how phosphorylation influences the structure of the H1 CTD remains unclear for many H1s. Using novel mass spectrometry techniques, we identified six phosphorylation sites within the CTD of the archetypal linker histone Xenopus H1.0. We then analyzed nucleosome-dependent CTD condensation and H1-dependent linker DNA organization for H1.0 in which the phosphorylated serine residues were replaced by glutamic acid residues (phosphomimics) in six independent mutants. We find that phosphomimetics at residues S117E, S155E, S181E, S188E, and S192E resulted in a significant reduction in nucleosome-bound H1.0 CTD condensation compared with unphosphorylated H1.0, whereas S130E did not alter CTD structure. Furthermore, we found distinct effects among the phosphomimetics on H1-dependent linker DNA trajectory, indicating unique mechanisms by which this modification can influence H1 CTD condensation. These results bring to light a novel role for linker histone phosphorylation in directly altering the structure of nucleosome-bound H1 and a potential novel mechanism for its effects on chromatin structure and function.
Alexandrow,
Chromatin decondensation in S-phase involves recruitment of Cdk2 by Cdc45 and histone H1 phosphorylation.
2005, Pubmed
Alexandrow,
Chromatin decondensation in S-phase involves recruitment of Cdk2 by Cdc45 and histone H1 phosphorylation.
2005,
Pubmed
Andrés,
Histone H1 Post-Translational Modifications: Update and Future Perspectives.
2020,
Pubmed
Bednar,
Structure and Dynamics of a 197 bp Nucleosome in Complex with Linker Histone H1.
2017,
Pubmed
,
Xenbase
Blom,
Prediction of post-translational glycosylation and phosphorylation of proteins from the amino acid sequence.
2004,
Pubmed
Blom,
Sequence and structure-based prediction of eukaryotic protein phosphorylation sites.
1999,
Pubmed
Bradbury,
Studies on the role and mode of operation of the very-lysine-rich histone H1 (F1) in eukaryote chromatin. The conformation of histone H1.
1975,
Pubmed
Carruthers,
Linker histones stabilize the intrinsic salt-dependent folding of nucleosomal arrays: mechanistic ramifications for higher-order chromatin folding.
1998,
Pubmed
Caterino,
Nucleosome linker DNA contacts and induces specific folding of the intrinsically disordered H1 carboxyl-terminal domain.
2011,
Pubmed
,
Xenbase
Clark,
Alpha-helix in the carboxy-terminal domains of histones H1 and H5.
1988,
Pubmed
Clark,
Electrostatic mechanism of chromatin folding.
1990,
Pubmed
Contreras,
The dynamic mobility of histone H1 is regulated by cyclin/CDK phosphorylation.
2003,
Pubmed
Cutter,
A brief review of nucleosome structure.
2015,
Pubmed
Dou,
The H1 phosphorylation state regulates expression of CDC2 and other genes in response to starvation in Tetrahymena thermophila.
2005,
Pubmed
Fan,
Histone H1 depletion in mammals alters global chromatin structure but causes specific changes in gene regulation.
2005,
Pubmed
Fang,
DNA and nucleosomes direct distinct folding of a linker histone H1 C-terminal domain.
2012,
Pubmed
,
Xenbase
Fang,
Chromatin structure-dependent conformations of the H1 CTD.
2016,
Pubmed
Grigoryev,
Hierarchical looping of zigzag nucleosome chains in metaphase chromosomes.
2016,
Pubmed
Gupta,
Identification of Posttranslational Modifications of Endogenous Chromatin Proteins From Testicular Cells by Mass Spectrometry.
2017,
Pubmed
Gutiyama,
Histone H1 of Trypanosoma cruzi is concentrated in the nucleolus region and disperses upon phosphorylation during progression to mitosis.
2008,
Pubmed
Hansen,
Intrinsic protein disorder, amino acid composition, and histone terminal domains.
2006,
Pubmed
Hao,
Acetylation-modulated communication between the H3 N-terminal tail domain and the intrinsically disordered H1 C-terminal domain.
2020,
Pubmed
Hao,
Unraveling linker histone interactions in nucleosomes.
2021,
Pubmed
Harshman,
H1 histones: current perspectives and challenges.
2013,
Pubmed
Hayes,
In vitro reconstitution and analysis of mononucleosomes containing defined DNAs and proteins.
1997,
Pubmed
,
Xenbase
Healton,
H1 linker histones silence repetitive elements by promoting both histone H3K9 methylation and chromatin compaction.
2020,
Pubmed
Hendzel,
The C-terminal domain is the primary determinant of histone H1 binding to chromatin in vivo.
2004,
Pubmed
Izzo,
The role of linker histone H1 modifications in the regulation of gene expression and chromatin dynamics.
2016,
Pubmed
Kavi,
Independent Biological and Biochemical Functions for Individual Structural Domains of Drosophila Linker Histone H1.
2016,
Pubmed
Koutzamani,
Linker histone subtype composition and affinity for chromatin in situ in nucleated mature erythrocytes.
2002,
Pubmed
,
Xenbase
Lever,
Rapid exchange of histone H1.1 on chromatin in living human cells.
2000,
Pubmed
Lopez,
Linker histone partial phosphorylation: effects on secondary structure and chromatin condensation.
2015,
Pubmed
Lu,
Linker histone H1 is essential for Drosophila development, the establishment of pericentric heterochromatin, and a normal polytene chromosome structure.
2009,
Pubmed
Lu,
Identification of specific functional subdomains within the linker histone H10 C-terminal domain.
2004,
Pubmed
Lu,
Revisiting the structure and functions of the linker histone C-terminal tail domain.
2003,
Pubmed
Maeshima,
Nucleosomal arrays self-assemble into supramolecular globular structures lacking 30-nm fibers.
2016,
Pubmed
Majumdar,
Measurements of internal distance changes of the 30S ribosome using FRET with multiple donor-acceptor pairs: quantitative spectroscopic methods.
2005,
Pubmed
Meyer,
From crystal and NMR structures, footprints and cryo-electron-micrographs to large and soft structures: nanoscale modeling of the nucleosomal stem.
2011,
Pubmed
Mishra,
A nucleosome-free region locally abrogates histone H1-dependent restriction of linker DNA accessibility in chromatin.
2018,
Pubmed
,
Xenbase
Mishra,
Spermatid-specific linker histone HILS1 is a poor condenser of DNA and chromatin and preferentially associates with LINE-1 elements.
2018,
Pubmed
Mishra,
Acetylation Mimics Within a Single Nucleosome Alter Local DNA Accessibility In Compacted Nucleosome Arrays.
2016,
Pubmed
,
Xenbase
Mishra,
Mapping of post-translational modifications of spermatid-specific linker histone H1-like protein, HILS1.
2015,
Pubmed
Murphy,
HMGN1 and 2 remodel core and linker histone tail domains within chromatin.
2017,
Pubmed
,
Xenbase
Pepenella,
A distinct switch in interactions of the histone H4 tail domain upon salt-dependent folding of nucleosome arrays.
2014,
Pubmed
,
Xenbase
Raghuram,
Pin1 promotes histone H1 dephosphorylation and stabilizes its binding to chromatin.
2013,
Pubmed
,
Xenbase
Roque,
DNA-induced secondary structure of the carboxyl-terminal domain of histone H1.
2005,
Pubmed
Roque,
Phosphorylation of the carboxy-terminal domain of histone H1: effects on secondary structure and DNA condensation.
2008,
Pubmed
Roth,
Chromatin condensation: does histone H1 dephosphorylation play a role?
1992,
Pubmed
Sarg,
Histone H1 phosphorylation occurs site-specifically during interphase and mitosis: identification of a novel phosphorylation site on histone H1.
2006,
Pubmed
Sarg,
Identification of novel post-translational modifications in linker histones from chicken erythrocytes.
2015,
Pubmed
Snijders,
Characterization of post-translational modifications of the linker histones H1 and H5 from chicken erythrocytes using mass spectrometry.
2008,
Pubmed
Strickfaden,
Reflections on the organization and the physical state of chromatin in eukaryotic cells.
2021,
Pubmed
Subirana,
Analysis of the charge distribution in the C-terminal region of histone H1 as related to its interaction with DNA.
,
Pubmed
Syed,
Single-base resolution mapping of H1-nucleosome interactions and 3D organization of the nucleosome.
2010,
Pubmed
Talasz,
In vivo phosphorylation of histone H1 variants during the cell cycle.
1996,
Pubmed
Th'ng,
Inhibition of histone phosphorylation by staurosporine leads to chromosome decondensation.
1994,
Pubmed
Thiriet,
Linker histone phosphorylation regulates global timing of replication origin firing.
2009,
Pubmed
Vila,
DNA-induced alpha-helical structure in the NH2-terminal domain of histone H1.
2001,
Pubmed
Willcockson,
H1 histones control the epigenetic landscape by local chromatin compaction.
2021,
Pubmed
Zhou,
Distinct Structures and Dynamics of Chromatosomes with Different Human Linker Histone Isoforms.
2021,
Pubmed