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J Cell Biol
2022 Mar 07;2213:. doi: 10.1083/jcb.202109016.
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A loop extrusion-independent mechanism contributes to condensin I-mediated chromosome shaping.
Kinoshita K
,
Tsubota Y
,
Tane S
,
Aizawa Y
,
Sakata R
,
Takeuchi K
,
Shintomi K
,
Nishiyama T
,
Hirano T
.
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Condensin I is a five-subunit protein complex that is central to mitotic chromosome assembly in eukaryotic cells. Despite recent progress, its molecular mechanisms of action remain to be fully elucidated. By using Xenopus egg extracts as a functional assay, we find that condensin I complexes harboring mutations in its kleisin subunit CAP-H produce chromosomes with confined axes in the presence of topoisomerase IIα (topo IIα) and highly compact structures (termed "beans") with condensin-positive central cores in its absence. The bean phenotype depends on the SMC ATPase cycle and can be reversed by subsequent addition of topo IIα. The HEAT repeat subunit CAP-D2, but not CAP-G, is essential for the bean formation. Notably, loop extrusion activities of the mutant complexes cannot explain the chromosomal defects they exhibit in Xenopus egg extracts, implying that a loop extrusion-independent mechanism contributes to condensin I-mediated chromosome assembly and shaping. We provide evidence that condensin-condensin interactions underlie these processes.
Alipour,
Self-organization of domain structures by DNA-loop-extruding enzymes.
2012, Pubmed
Alipour,
Self-organization of domain structures by DNA-loop-extruding enzymes.
2012,
Pubmed
Bayliss,
Structural basis for the interaction between FxFG nucleoporin repeats and importin-beta in nuclear trafficking.
2000,
Pubmed
Bayliss,
GLFG and FxFG nucleoporins bind to overlapping sites on importin-beta.
2002,
Pubmed
Cheng,
A simple biophysical model emulates budding yeast chromosome condensation.
2015,
Pubmed
Davidson,
DNA loop extrusion by human cohesin.
2019,
Pubmed
Eng,
Interallelic complementation provides functional evidence for cohesin-cohesin interactions on DNA.
2015,
Pubmed
Ganji,
Real-time imaging of DNA loop extrusion by condensin.
2018,
Pubmed
Gerguri,
Comparison of loop extrusion and diffusion capture as mitotic chromosome formation pathways in fission yeast.
2021,
Pubmed
Gibcus,
A pathway for mitotic chromosome formation.
2018,
Pubmed
,
Xenbase
Goloborodko,
Compaction and segregation of sister chromatids via active loop extrusion.
2016,
Pubmed
Goloborodko,
Chromosome Compaction by Active Loop Extrusion.
2016,
Pubmed
Hara,
Structural basis of HEAT-kleisin interactions in the human condensin I subcomplex.
2019,
Pubmed
,
Xenbase
Hassler,
Structural Basis of an Asymmetric Condensin ATPase Cycle.
2019,
Pubmed
Hirano,
Condensins and the evolution of torsion-mediated genome organization.
2014,
Pubmed
Hirano,
Condensin-Based Chromosome Organization from Bacteria to Vertebrates.
2016,
Pubmed
Hirano,
Topoisomerase II does not play a scaffolding role in the organization of mitotic chromosomes assembled in Xenopus egg extracts.
1993,
Pubmed
,
Xenbase
Hirano,
A heterodimeric coiled-coil protein required for mitotic chromosome condensation in vitro.
1994,
Pubmed
,
Xenbase
Hirano,
Condensins, chromosome condensation protein complexes containing XCAP-C, XCAP-E and a Xenopus homolog of the Drosophila Barren protein.
1997,
Pubmed
,
Xenbase
Keenholtz,
Oligomerization and ATP stimulate condensin-mediated DNA compaction.
2017,
Pubmed
Kim,
Human cohesin compacts DNA by loop extrusion.
2019,
Pubmed
Kinoshita,
Balancing acts of two HEAT subunits of condensin I support dynamic assembly of chromosome axes.
2015,
Pubmed
,
Xenbase
Kong,
Human Condensin I and II Drive Extensive ATP-Dependent Compaction of Nucleosome-Bound DNA.
2020,
Pubmed
Kschonsak,
Structural Basis for a Safety-Belt Mechanism That Anchors Condensin to Chromosomes.
2017,
Pubmed
Lee,
Condensins I and II are essential for construction of bivalent chromosomes in mouse oocytes.
2011,
Pubmed
Lee,
Cryo-EM structures of holo condensin reveal a subunit flip-flop mechanism.
2020,
Pubmed
Liu,
Structural basis for the high-affinity binding of nucleoporin Nup1p to the Saccharomyces cerevisiae importin-beta homologue, Kap95p.
2005,
Pubmed
Lord,
SuperPlots: Communicating reproducibility and variability in cell biology.
2020,
Pubmed
Losada,
Identification of Xenopus SMC protein complexes required for sister chromatid cohesion.
1998,
Pubmed
,
Xenbase
Nasmyth,
Disseminating the genome: joining, resolving, and separating sister chromatids during mitosis and meiosis.
2001,
Pubmed
Ono,
Differential contributions of condensin I and condensin II to mitotic chromosome architecture in vertebrate cells.
2003,
Pubmed
,
Xenbase
Paulson,
Mitotic chromosomes.
2021,
Pubmed
Piazza,
Association of condensin with chromosomes depends on DNA binding by its HEAT-repeat subunits.
2014,
Pubmed
Ryu,
The condensin holocomplex cycles dynamically between open and collapsed states.
2020,
Pubmed
Ryu,
Bridging-induced phase separation induced by cohesin SMC protein complexes.
2021,
Pubmed
Sakai,
Modeling the functions of condensin in chromosome shaping and segregation.
2018,
Pubmed
Sakata,
Opening of cohesin's SMC ring is essential for timely DNA replication and DNA loop formation.
2021,
Pubmed
Shintomi,
Guiding functions of the C-terminal domain of topoisomerase IIα advance mitotic chromosome assembly.
2021,
Pubmed
,
Xenbase
Shintomi,
Reconstitution of mitotic chromatids with a minimum set of purified factors.
2015,
Pubmed
,
Xenbase
Shintomi,
Mitotic chromosome assembly despite nucleosome depletion in Xenopus egg extracts.
2017,
Pubmed
,
Xenbase
Tedeschi,
Wapl is an essential regulator of chromatin structure and chromosome segregation.
2013,
Pubmed
Terakawa,
The condensin complex is a mechanochemical motor that translocates along DNA.
2017,
Pubmed
Uhlmann,
SMC complexes: from DNA to chromosomes.
2016,
Pubmed
Xiang,
Cohesin architecture and clustering in vivo.
2021,
Pubmed
Yoshimura,
HEAT repeats - versatile arrays of amphiphilic helices working in crowded environments?
2016,
Pubmed
Yoshimura,
Structural mechanism of nuclear transport mediated by importin β and flexible amphiphilic proteins.
2014,
Pubmed