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FIGURE 1. Phylogenetic tree showing relationships of alpha 7 nAChR subunit protein sequence of the cockroach P. americana and its orthologs in several insect species and human. The bootstrap support value (%) based on 1,000 replicates are shown when higher than 50%. The D. melanogaster GABAA subunit (accession number AAA28556.1) was used as outgroup. Accession sequence identifiers are as follows: Anopheles gambiae: Agamα1 (AAU12503.1), Agamα2 (AAU12504.1), Agamα3 (XP_310786.3), Agamα4 (XP_566274.3), Agamα5 (XP_314691.2), Agamα6 (XP_308042.3), Agamα7 (XP_309153.3), Agamα8 (XP_311925.3), Agamα9 (XP_310203.3), Agamβ1 (XP_309158.3); Aphis gossypii: Agosα1 (AAM94383.1), Agosα2 (AAM94382.1), Agosα3 (ABR21379.1), Agosα4 (ABR21380.1), Agosα7 (AFM78640.1), Agosα8 (BBA21164.1), Agosβ1 (AAM94384.1); Apis mellifera: Amelα1 (NP_001091690.1), Amelα2 (NP_001011625.1), Amelα3 (NP_001073029.1), Amelα4 (NP_001091691.1), Amelα5 (AJE70263.1), Amelα6 (NP_001073564.1), Amelα7 (AJE70265.1), Amelα8 (NP_001011575.1), Amelα9 (NP_001091694.1), Amelα10 (XP_392070.3), Amelβ1 (NP_001073028.1), Amelβ2 (NP_001091699.1); Bactrocera oleae: Boleα2 (XP_014100082.1), Boleα7 (XP_014102300.1), Boleβ2 (XP_0111204502.1), Boleβ3 (XP_014090987.1); Bombyx mori: Bmorα1 (ABV45511.1), Bmorα2 (ABV45512.1), Bmorα3 (ABV45513.1), Bmorα4 (ABV45514.1), Bmorα5 (ABV45516.1), Bmorα6 (NP_001091830.1), Bmorα7 (ABV45520.2), Bmorα8 (ABV45521.1), Bmorα9 (ABV45523.1), Bmorβ1 (NP_001166819.1), Bmorβ2 (NP_001103400.1), Bmorβ3 (NP_00110341.1); Ceratitis capitata: Ccapα2 (XP_004536261.1), Ccapα7 (JAB87466.1), Ccapβ1 (XP_012156453.1), Ccapβ2 (XP_012162675.1); Drosophila melanogaster: Dmelα1 (CAA30172.1), Dmelα2 (NP_524482.1), Dmelα3 (CAA75688.1), Dmelα4 (CAB77445.1), Dmelα5 (AAM13390.1), Dmelα6 (NP_723494.2), Dmelα7 (CAD86936.1), Dmelβ1 (P04755.1), Dmelβ2 (CAA39211.1), Dmelβ3 (NP_525098.1); Musca domestica: Mdomα2 (ABD37617.1), Mdomα5 (ABY40460.1), Mdomα6 (ABJ09669.1), Mdomβ1 (XP_005180169.1), Mdomβ2 (XP_005185796.1), Mdomβ3 (ABY40465.1); Myzus persicae: Mperα3 (CAB52297.1), Mperβ1 (XP_022165274.1), Mperβ2 (XP_022167599.1); Nasonia vitripennis: Nvitα1 (ACY82683.1), Nvitα2 (ACY82684.1), Nvitα3 (ACY82685.1), Nvitα4 (ACY82686.1), Nvitα5 (ACY82688.1), Nvitα6 (ACY82689.1), Nvitα7 (ACY82692.1), Nvitα8 (ACY82693.1), Nvitα9 (ACY82694.1), Nvitα10 (ACY82695.1), Nvitα11 (ACY82696.1), Nvitα12 (ACY82697.1), Nvitβ1 (ACY82698.1), Nvitβ2 (ACY82699.1), Nvitβ3 (ACY82700.1); Nilaparvata lugens; Nlugα1 (AAQ75737.1), Nlugα2 (AAQ7574101), Nlugα3 (AAQ75739.1), Nlugβ2 (AAQ75742.2); Periplaneta americana: Pameα1 (AKV94620.1), Pameα2 (AKV94621.1), Pameα3 (AKR16132.1), Pameα4 (AFA28129.1), Pameα6 (AKV94622.1), Pameα7 (MK790056), Pameα8 (AFA28130.1), Pameβ1 (AKV94624.1); Tribolium castaneum: Tcasα1 (ABS86902.1), Tcasα2 (ABS86903.1), Tcasα3 (ABS86904.1), Tcasα4 (ABS86905.1), Tcasα5 (ABS86907.1), Tcasα6 (ABS86908.1), Tcasα7 (ABS86911.1), Tcasα8 (ABS86912.1), Tcasα9 (ABS86913.1), Tcasα10 (ABS86914.1), Tcasα11 (ABS86915.1), Tcasβ1 (ABS86916.1); Homo sapiens: Hsapα2 (AAB40109.1), Hsapα3 (AAA59942.1), Hsapα4 (AAA64743.1), Hsapα5 (AAA58357.1), Hsapα6 (AAB40113.1), Hsapα7 (CAA49778.1), Hsapα9 (CAB65091.1), Hsapα10 (CAC20435.1), Hsapβ2 (CAA37320.1), Hsapβ3 (CAA47851.1), Hsapβ4 (CAA48336.1); Ctenocephalides felis: Cfelα1 (ABB42999), Cfelα2 (ABB43000), Cfelα3 (ABB43001), Cfelα4 (ABB43003), Cfelα7 (ABB43004), Cfelα8 (ABB43002), Cfelβ1 (ABB43005).
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