XB-ART-5655
Chem Biol
2003 Feb 01;102:131-7. doi: 10.1016/s1074-5521(03)00020-6.
Show Gene links
Show Anatomy links
A novel action of terpendole E on the motor activity of mitotic Kinesin Eg5.
???displayArticle.abstract???
To reveal the mechanism of mitosis, the development of M phase-specific inhibitors is an important strategy. We have been screening microbial products to find specific M phase inhibitors that do not directly target tubulins, and rediscovered terpendole E (TerE) as a novel Eg5 inhibitor. TerE did not affect microtubule integrity in interphase, but induced formation of a monoastral spindle in M phase. TerE inhibited both motor and microtubule-stimulated ATPase activities of human Eg5, but did not affect conventional kinesin from either Drosophila or bovine brain. Although terpendoles have been reported as inhibitors of acyl-CoA:cholesterol O-acyltransferase (ACAT), the Eg5 inhibitory activity of TerE was independent of ACAT inhibition. Taken together, we demonstrate that TerE is a novel Eg5 inhibitor isolated from a fungal strain.
???displayArticle.pubmedLink??? 12618185
???displayArticle.link??? Chem Biol
Species referenced: Xenopus
Genes referenced: ces2.8 kif11