Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Mol Biol Cell
2003 Mar 01;143:1255-67. doi: 10.1091/mbc.e02-09-0601.
Show Gene links
Show Anatomy links
Subnuclear localization and Cajal body targeting of transcription elongation factor TFIIS in amphibian oocytes.
Smith AJ
,
Ling Y
,
Morgan GT
.
???displayArticle.abstract???
We have examined the localization and targeting of the RNA polymerase II (pol II) transcription elongation factor TFIIS in amphibian oocyte nuclei by immunofluorescence. Using a novel antibody against Xenopus TFIIS the major sites of immunostaining were found to be Cajal bodies, nuclear organelles that also contain pol II. Small granular structures attached to lampbrush chromosomes were also specifically stained but the transcriptionally active loops were not. Similar localization patterns were found for the newly synthesized myc-tagged TFIIS produced after injection of synthetic transcripts into the cytoplasm. The basis of the rapid and preferential targeting of TFIIS to Cajal bodies was investigated by examining the effects of deletion and site-specific mutations. Multiple regions of TFIIS contributed to efficient targeting including the domain required for its binding to pol II. The localization of TFIIS in Cajal bodies, and in particular the apparent involvement of pol II binding in achieving it, offer further support for a model in which Cajal bodies function in the preassembly of the transcriptional machinery. Although our findings are therefore consistent with TFIIS playing a role in early events of the transcription cycle, they also suggest that this elongation factor is not generally required during transcription in oocytes.
Abbott,
The stem-loop binding protein (SLBP1) is present in coiled bodies of the Xenopus germinal vesicle.
1999, Pubmed,
Xenbase
Abbott,
The stem-loop binding protein (SLBP1) is present in coiled bodies of the Xenopus germinal vesicle.
1999,
Pubmed
,
Xenbase
Awrey,
Yeast transcript elongation factor (TFIIS), structure and function. II: RNA polymerase binding, transcript cleavage, and read-through.
1998,
Pubmed
Booth,
Structure of a conserved domain common to the transcription factors TFIIS, elongin A, and CRSP70.
2000,
Pubmed
Bucci,
TAFII70 protein in Cajal bodies of the amphibian germinal vesicle.
2001,
Pubmed
Cipres-Palacin,
Alanine-scanning mutagenesis of human transcript elongation factor TFIIS.
1995,
Pubmed
Doyle,
The distribution of RNA polymerase II largest subunit (RPB1) in the Xenopus germinal vesicle.
2002,
Pubmed
,
Xenbase
Dundr,
Functional architecture in the cell nucleus.
2001,
Pubmed
Evan,
Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product.
1985,
Pubmed
,
Xenbase
Gall,
Lampbrush chromosomes.
1991,
Pubmed
,
Xenbase
Gall,
Cajal bodies: the first 100 years.
2000,
Pubmed
Gall,
Assembly of lampbrush chromosomes from sperm chromatin.
1998,
Pubmed
,
Xenbase
Gall,
Assembly of the nuclear transcription and processing machinery: Cajal bodies (coiled bodies) and transcriptosomes.
1999,
Pubmed
,
Xenbase
Gall,
Is the sphere organelle/coiled body a universal nuclear component?
1995,
Pubmed
,
Xenbase
Hirose,
RNA polymerase II and the integration of nuclear events.
2000,
Pubmed
Ishiguro,
The Rpb6 subunit of fission yeast RNA polymerase II is a contact target of the transcription elongation factor TFIIS.
2000,
Pubmed
Jantsch,
Assembly and localization of the U1-specific snRNP C protein in the amphibian oocyte.
1992,
Pubmed
,
Xenbase
Labhart,
Identification of novel genes encoding transcription elongation factor TFIIS (TCEA) in vertebrates: conservation of three distinct TFIIS isoforms in frog, mouse, and human.
1998,
Pubmed
,
Xenbase
Labhart,
Transcript cleavage in an RNA polymerase I elongation complex. Evidence for a dissociable activity similar to but distinct from TFIIS.
1997,
Pubmed
,
Xenbase
Lennon,
Mutations in RNA polymerase II and elongation factor SII severely reduce mRNA levels in Saccharomyces cerevisiae.
1998,
Pubmed
Matera,
Nuclear bodies: multifaceted subdomains of the interchromatin space.
1999,
Pubmed
Miller,
Visualization of RNA synthesis on chromosomes.
1972,
Pubmed
Misteli,
Protein dynamics: implications for nuclear architecture and gene expression.
2001,
Pubmed
Morgan,
RNA polymerase II in Cajal bodies of amphibian oocytes.
2000,
Pubmed
,
Xenbase
Morgan,
Lampbrush chromosomes and associated bodies: new insights into principles of nuclear structure and function.
2002,
Pubmed
Morin,
Elongation factor TFIIS contains three structural domains: solution structure of domain II.
1996,
Pubmed
Murphy,
RNA polymerase III in Cajal bodies and lampbrush chromosomes of the Xenopus oocyte nucleus.
2002,
Pubmed
,
Xenbase
Myer,
RNA polymerase II holoenzymes and subcomplexes.
1998,
Pubmed
Olmsted,
Yeast transcript elongation factor (TFIIS), structure and function. I: NMR structural analysis of the minimal transcriptionally active region.
1998,
Pubmed
Pan,
Interaction of elongation factors TFIIS and elongin A with a human RNA polymerase II holoenzyme capable of promoter-specific initiation and responsive to transcriptional activators.
1997,
Pubmed
Plant,
Genes encoding isoforms of transcription elongation factor TFIIS in Xenopus and the use of multiple unusual RNA processing signals.
1996,
Pubmed
,
Xenbase
Pokholok,
Exchange of RNA polymerase II initiation and elongation factors during gene expression in vivo.
2002,
Pubmed
Qian,
Novel zinc finger motif in the basal transcriptional machinery: three-dimensional NMR studies of the nucleic acid binding domain of transcriptional elongation factor TFIIS.
1993,
Pubmed
Robert,
A human RNA polymerase II-containing complex associated with factors necessary for spliceosome assembly.
2002,
Pubmed
Scheer,
Classification of loops of lampbrush chromosomes according to the arrangement of transcriptional complexes.
1976,
Pubmed
Shimasaki,
Structural basis for the species-specific activity of TFIIS.
2000,
Pubmed
Taira,
Molecular cloning of cDNA and tissue-specific expression of the gene for SII-K1, a novel transcription elongation factor SII.
1998,
Pubmed
Taira,
Participation of transcription elongation factor XSII-K1 in mesoderm-derived tissue development in Xenopus laevis.
2000,
Pubmed
,
Xenbase
Tuma,
Identification and characterization of a sphere organelle protein.
1993,
Pubmed
,
Xenbase
Warren,
Cytostellin: a novel, highly conserved protein that undergoes continuous redistribution during the cell cycle.
1992,
Pubmed
Wind,
Transcription elongation factor SII.
2000,
Pubmed
Wu,
In vitro characterization of mutant yeast RNA polymerase II with reduced binding for elongation factor TFIIS.
1996,
Pubmed
Wu,
Human p80-coilin is targeted to sphere organelles in the amphibian germinal vesicle.
1994,
Pubmed
,
Xenbase
