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Figure 1. Effects of CuSO4 and Bordeaux mixture exposures on X. laevis oocyte’s maturation. Oocytes were exposed to increasing concentrations
of CuSO4 (A, C) or Bordeaux mixture (B, D) in presence (A, B) or not (C, D) of progesterone (Pg) for 13 h. Every 15 minutes,
the maturation was assessed according to the white spot appearance. Results are expressed as mean ± SEM (grey areas) and compared
to other treatments using Friedman rank sum test (P<0.05).
Figure 2. Effects of CuSO4 exposures on eye gap in 6-days old tadpoles. Tadpoles were
exposed increasing concentrations of CuSO4 from the fertilization. After alcian blue
staining, automatic image analysis was performed under Image J software (see Slaby et
al., 2016, for details). Results are expressed as boxplots and compared to others using
Kruskal-Wallis rank sum test (P<0.01).
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