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Changes in villin synthesis and subcellular distribution during intestinal differentiation of HT29-18 clones.
Dudouet B
,
Robine S
,
Huet C
,
Sahuquillo-Merino C
,
Blair L
,
Coudrier E
,
Louvard D
.
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Brush border in enterocytes is a cell surface specialization intimately associated with terminal differentiation of these cells. HT29-18, a clone derived from the HT-29 human colonic adenocarcinoma cell line, and HT29-18-C1, a subclone from HT29-18 described in the companion paper (Huet, C., C. Sahuquillo-Merino, E. Coudrier, and D. Louvard, 1987, J. Cell Biol., 105:345-357), undergo terminal differentiation with brush borders in the absence of glucose or upon replacement of glucose by galactose in the medium. Taking advantage of this clone and its subclone which can be manipulated in vitro, we have studied the synthesis and subcellular distribution of villin, one major protein in the microvillus core of the brush border. For this study, a monoclonal antibody against villin (BDID2C3) has been isolated and characterized in detail. In addition an ELISA has been set up to measure villin accurately in total cell extracts. Villin content in differentiated HT29-18 cells is close to that seen in normal human colonic cells but 10 times lower in undifferentiated HT29-18 cells. The rate of villin synthesis is dramatically increased in the course of enterocytic differentiation, while villin is remarkably stable after synthesis. We have recently shown, using a cDNA probe for villin, that this change is controlled either at the transcription level or by RNA stabilization (Pringault, E., M. Arpin, A. Garcia, J. Finidori, and D. Louvard, 1986, EMBO (Eur. Mol. Biol. Organ.) J., 5:3119-3124). As shown by immunofluorescence and immunogold labelings, villin is targeted to the brush border area of differentiated HT29-18 cells but remains diffusely distributed in undifferentiated ones.
Armbruster,
Specimen preparation for electron microscopy using low temperature embedding resins.
1982, Pubmed
Armbruster,
Specimen preparation for electron microscopy using low temperature embedding resins.
1982,
Pubmed
Blikstad,
Synthesis and assembly of spectrin during avian erythropoiesis: stoichiometric assembly but unequal synthesis of alpha and beta spectrin.
1983,
Pubmed
Bretscher,
Villin: the major microfilament-associated protein of the intestinal microvillus.
1979,
Pubmed
Bretscher,
Villin is a major protein of the microvillus cytoskeleton which binds both G and F actin in a calcium-dependent manner.
1980,
Pubmed
Bretscher,
Villin associates with specific microfilamentous structures as seen by immunofluorescence microscopy on tissue sections and cells microinjected with villin.
1981,
Pubmed
Burnette,
"Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A.
1981,
Pubmed
Coudrier,
Characterization of an integral membrane glycoprotein associated with the microfilaments of pig intestinal microvilli.
1983,
Pubmed
Craig,
Comparison of intestinal brush-border 95-Kdalton polypeptide and alpha-actinins.
1980,
Pubmed
Craig,
Regulation of actin polymerization by villin, a 95,000 dalton cytoskeletal component of intestinal brush borders.
1980,
Pubmed
Drenckhahn,
Evidence for the association of villin with core filaments and rootlets of intestinal epithelial microvilli.
1983,
Pubmed
Garrels,
Identification and characterization of multiple forms of actin.
1976,
Pubmed
Gerke,
Isolation and characterization of mammalian villin and fimbrin, the two bundling proteins of the intestinal microvilli.
1983,
Pubmed
Glenney,
F actin assembly modulated by villin: Ca++-dependent nucleation and capping of the barbed end.
1981,
Pubmed
Glenney,
Calcium control of microfilaments: uncoupling of the F-actin-severing and -bundling activity of villin by limited proteolysis in vitro.
1981,
Pubmed
Glenney,
Demonstration of at least two different actin-binding sites in villin, a calcium-regulated modulator of F-actin organization.
1981,
Pubmed
Guesdon,
Magnetic enzyme immunoassay for measuring human IgE.
1978,
Pubmed
Gustin,
Isolation of brush-border membrane from the rabbit descending colon epithelium. Partial characterization of a unique K+-activated ATPase.
1981,
Pubmed
Herzlinger,
The MDCK epithelial cell line expresses a cell surface antigen of the kidney distal tubule.
1982,
Pubmed
Hesterberg,
Ligand-induced conformational changes in villin, a calcium-controlled actin-modulating protein.
1983,
Pubmed
Hesterberg,
Demonstration of three distinct calcium-binding sites in villin, a modulator of actin assembly.
1983,
Pubmed
Howe,
A sensitive immunoblotting method for measuring protein synthesis initiation factor levels in lysates of Escherichia coli.
1981,
Pubmed
Huet,
Absorptive and mucus-secreting subclones isolated from a multipotent intestinal cell line (HT-29) provide new models for cell polarity and terminal differentiation.
1987,
Pubmed
Koprowski,
Production of antibodies against influenza virus by somatic cell hybrids between mouse myeloma and primed spleen cells.
1977,
Pubmed
Köhler,
Continuous cultures of fused cells secreting antibody of predefined specificity.
1975,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Lazarides,
Assembly and topogenesis of the spectrin-based membrane skeleton in erythroid development.
1984,
Pubmed
Matsudaira,
Partial reconstruction of the microvillus core bundle: characterization of villin as a Ca++-dependent, actin-bundling/depolymerizing protein.
1982,
Pubmed
Matsudaira,
Organization of the cross-filaments in intestinal microvilli.
1982,
Pubmed
Matsudaira,
A gelsolin-like Ca2+-dependent actin-binding domain in villin.
,
Pubmed
McLean,
Periodate-lysine-paraformaldehyde fixative. A new fixation for immunoelectron microscopy.
1974,
Pubmed
Moon,
Biogenesis of the avian erythroid membrane skeleton: receptor-mediated assembly and stabilization of ankyrin (goblin) and spectrin.
1984,
Pubmed
Mooseker,
Organization, chemistry, and assembly of the cytoskeletal apparatus of the intestinal brush border.
1985,
Pubmed
Mooseker,
Regulation of microvillus structure: calcium-dependent solation and cross-linking of actin filaments in the microvilli of intestinal epithelial cells.
1980,
Pubmed
Mostov,
Biosynthesis, processing, and function of secretory component.
1983,
Pubmed
Pringault,
A human villin cDNA clone to investigate the differentiation of intestinal and kidney cells in vivo and in culture.
1986,
Pubmed
REYNOLDS,
The use of lead citrate at high pH as an electron-opaque stain in electron microscopy.
1963,
Pubmed
Rabito,
Na+-dependent sugar transport in a cultured epithelial cell line from pig kidney.
1980,
Pubmed
Reggio,
Surface and cytoplasmic domains in polarized epithelial cells.
1982,
Pubmed
Reggio,
Use of immunocytochemical techniques in studying the biogenesis of cell surfaces in polarized epithelia.
1983,
Pubmed
Robine,
Can villin be used to identify malignant and undifferentiated normal digestive epithelial cells?
1985,
Pubmed
Shibayama,
Assembly of the intestinal brush border: appearance and redistribution of microvillar core proteins in developing chick enterocytes.
1987,
Pubmed
Woods,
Degradation of unassembled alpha- and beta-spectrin by distinct intracellular pathways: regulation of spectrin topogenesis by beta-spectrin degradation.
1985,
Pubmed
