XB-ART-55083
Cold Spring Harb Protoc
2019 May 01;20195:. doi: 10.1101/pdb.prot097592.
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Adoptive Transfer of Fluorescently Labeled Immune Cells in Xenopus.
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Adoptive cell transfer from inbred adult Xenopus to inbred tadpoles is a useful way to study the dissemination of immune cells or pathogen-infected immune cells in tadpoles. For example, Xenopus peritoneal leukocytes (PLs) can be readily infected by pathogens such as Frog virus 3 (FV3) and Mycobacterium marinum (M. marinum). By transferring fluorescently labeled, FV3-infected PLs into tadpoles, we observed infiltration of these cells into the tadpole's brain, which indicates that FV3-infected PLs can cross blood brain barrier. Taking advantage of tadpoles' transparency, fluorescently labeled immune cells can be tracked in real time using fluorescence microscopy.
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Species referenced: Xenopus laevis
References [+] :
De Jesús Andino,
Frog Virus 3 dissemination in the brain of tadpoles, but not in adult Xenopus, involves blood brain barrier dysfunction.
2016, Pubmed,
Xenbase