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Ann Bot
2018 Sep 24;1224:641-648. doi: 10.1093/aob/mcy103.
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Isolation and functional characterization of CsLsi2, a cucumber silicon efflux transporter gene.
Sun H
,
Duan Y
,
Qi X
,
Zhang L
,
Huo H
,
Gong H
.
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Background and Aims: Silicon has been proven to exert beneficial effects on plant growth and stress tolerance, and silicon accumulation varies among different plant species. Cucumber (Cucumis sativus) is a widely used dicot model for silicon accumulation, but little is known about the molecular mechanism of its silicon uptake. Previously, we isolated and characterized CsLsi1, a silicon influx transporter gene from cucumber. In this study, we cloned a putative silicon efflux transporter gene, CsLsi2, from cucumber and investigated its role in silicon uptake.
Methods: The expression pattern, transport activity, and subcellular and cellular localizations of CsLsi2 were investigated. The transport activity of CsLsi2 was determined in Xenopus laevis oocytes. The subcelluar and cellular localizations were conducted by transient expression of fused 35S::CsLsi2-eGFP in onion epidermal cells and expression of ProCsLsi2::CsLsi2-mGFP in cucumber, respectively.
Key Results: CsLsi2 was mainly expressed in the roots. Expression of CsLsi2-eGFP fusion sequence in onion epidermis cells showed that CsLsi2 was localized at the plasma membrane. Transient expression in Xenopus laevis oocytes showed that CsLsi2 demonstrated efflux but no influx transport activity for silicon, and the transport was energy-dependent. Expression of CsLsi2-mGFP under its own promoter revealed that CsLsi2 was mainly expressed on endodermal cells, showing no polar distribution. In combination with our previous work on CsLsi1, a model for silicon uptake in cucumber roots is proposed.
Conclusion: The results suggest that CsLsi2 is a silicon efflux transporter gene in cucumber. The coordination of CsLsi1 and CsLsi2 mediates silicon uptake in cucumber roots. This study may help us understand the molecular mechanism for silicon uptake in cucumber, one of the few dicots with a relatively high capacity for silicon accumulation.
Chiba,
HvLsi1 is a silicon influx transporter in barley.
2009, Pubmed,
Xenbase
Chiba,
HvLsi1 is a silicon influx transporter in barley.
2009,
Pubmed
,
Xenbase
Chou,
Plant-mPLoc: a top-down strategy to augment the power for predicting plant protein subcellular localization.
2010,
Pubmed
Debona,
Silicon's Role in Abiotic and Biotic Plant Stresses.
2017,
Pubmed
Deshmukh,
Identification and functional characterization of silicon transporters in soybean using comparative genomics of major intrinsic proteins in Arabidopsis and rice.
2013,
Pubmed
,
Xenbase
Epstein,
SILICON.
1999,
Pubmed
Hodson,
Phylogenetic variation in the silicon composition of plants.
2005,
Pubmed
Lee,
Exposure of roots of cucumber (Cucumis sativus) to low temperature severely reduces root pressure, hydraulic conductivity and active transport of nutrients.
2004,
Pubmed
Liang,
Silicon uptake and transport is an active process in Cucumis sativus.
2005,
Pubmed
Ma,
A cooperative system of silicon transport in plants.
2015,
Pubmed
Ma,
An efflux transporter of silicon in rice.
2007,
Pubmed
Ma,
A silicon transporter in rice.
2006,
Pubmed
Mitani,
Identification of the silicon form in xylem sap of rice (Oryza sativa L.).
2005,
Pubmed
Mitani,
Identification of maize silicon influx transporters.
2009,
Pubmed
,
Xenbase
Mitani,
Isolation and functional characterization of an influx silicon transporter in two pumpkin cultivars contrasting in silicon accumulation.
2011,
Pubmed
,
Xenbase
Mitani,
Identification and characterization of maize and barley Lsi2-like silicon efflux transporters reveals a distinct silicon uptake system from that in rice.
2009,
Pubmed
,
Xenbase
Mitani,
Uptake system of silicon in different plant species.
2005,
Pubmed
Mitani-Ueno,
Physiological and molecular characterization of Si uptake in wild rice species.
2014,
Pubmed
,
Xenbase
Mitani-Ueno,
Silicon efflux transporters isolated from two pumpkin cultivars contrasting in Si uptake.
2011,
Pubmed
Mitani-Ueno,
High Silicon Accumulation in the Shoot is Required for Down-Regulating the Expression of Si Transporter Genes in Rice.
2016,
Pubmed
Montpetit,
Cloning, functional characterization and heterologous expression of TaLsi1, a wheat silicon transporter gene.
2012,
Pubmed
,
Xenbase
Nikolic,
Germanium-68 as an adequate tracer for silicon transport in plants. Characterization of silicon uptake in different crop species.
2007,
Pubmed
Sakurai,
A Model of Silicon Dynamics in Rice: An Analysis of the Investment Efficiency of Si Transporters.
2017,
Pubmed
Sakurai,
In silico simulation modeling reveals the importance of the Casparian strip for efficient silicon uptake in rice roots.
2015,
Pubmed
Scott,
Model system for plant cell biology: GFP imaging in living onion epidermal cells.
1999,
Pubmed
Sun,
Isolation and functional characterization of CsLsi1, a silicon transporter gene in Cucumis sativus.
2017,
Pubmed
,
Xenbase
Tamura,
MEGA6: Molecular Evolutionary Genetics Analysis version 6.0.
2013,
Pubmed
Trembath-Reichert,
Four hundred million years of silica biomineralization in land plants.
2015,
Pubmed
Wan,
Selection of appropriate reference genes for gene expression studies by quantitative real-time polymerase chain reaction in cucumber.
2010,
Pubmed
