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Biophys J
2018 Jun 05;11411:2563-2572. doi: 10.1016/j.bpj.2018.04.014.
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Molecular Insights into Variable Electron Transfer in Amphibian Cryptochrome.
Sjulstok E
,
Lüdemann G
,
Kubař T
,
Elstner M
,
Solov'yov IA
.
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Cryptochrome proteins are activated by the absorption of blue light, leading to the formation of radical pairs through electron transfer in the active site. Recent experimental studies have shown that once some of the amino acid residues in the active site of Xenopus laevis cryptochrome DASH are mutated, radical-pair formation is still observed. In this study, we computationally investigate electron-transfer pathways in the X. laevis cryptochrome DASH by extensively equilibrating a previously established homology model using molecular dynamics simulations and then mutating key amino acids involved in the electron transfer. The electron-transfer pathways are then probed by using tight-binding density-functional theory. We report the alternative electron-transfer pathways resolved at the molecular level and, through comparison of amino acid sequences for cryptochromes from different species, we demonstrate that one of these alternative electron-transfer pathways could be general for all cryptochrome DASH proteins.
Ahmad,
Magnetic intensity affects cryptochrome-dependent responses in Arabidopsis thaliana.
2007, Pubmed
Ahmad,
Magnetic intensity affects cryptochrome-dependent responses in Arabidopsis thaliana.
2007,
Pubmed
Ahmad,
Seeing blue: the discovery of cryptochrome.
1996,
Pubmed
Apweiler,
UniProt: the Universal Protein knowledgebase.
2004,
Pubmed
Biskup,
Direct observation of a photoinduced radical pair in a cryptochrome blue-light photoreceptor.
2009,
Pubmed
Biskup,
Variable electron transfer pathways in an amphibian cryptochrome: tryptophan versus tyrosine-based radical pairs.
2013,
Pubmed
Brautigam,
Structure of the photolyase-like domain of cryptochrome 1 from Arabidopsis thaliana.
2004,
Pubmed
Brudler,
Identification of a new cryptochrome class. Structure, function, and evolution.
2003,
Pubmed
Cashmore,
Cryptochromes: blue light receptors for plants and animals.
1999,
Pubmed
Chaves,
The cryptochromes: blue light photoreceptors in plants and animals.
2011,
Pubmed
Cohen,
Challenges for density functional theory.
2012,
Pubmed
Czarna,
Structures of Drosophila cryptochrome and mouse cryptochrome1 provide insight into circadian function.
2013,
Pubmed
Dodson,
A radical sense of direction: signalling and mechanism in cryptochrome magnetoreception.
2013,
Pubmed
El-Esawi,
Cellular metabolites modulate in vivo signaling of Arabidopsis cryptochrome-1.
2015,
Pubmed
Emlen,
Celestial rotation: its importance in the development of migratory orientation.
1970,
Pubmed
Engelhard,
Cellular metabolites enhance the light sensitivity of Arabidopsis cryptochrome through alternate electron transfer pathways.
2014,
Pubmed
Friis,
Computational reconstruction reveals a candidate magnetic biocompass to be likely irrelevant for magnetoreception.
2017,
Pubmed
Gegear,
Cryptochrome mediates light-dependent magnetosensitivity in Drosophila.
2008,
Pubmed
Harris,
Effect of magnetic fields on cryptochrome-dependent responses in Arabidopsis thaliana.
2009,
Pubmed
Hein,
Robins have a magnetic compass in both eyes.
2011,
Pubmed
Hess,
GROMACS 4: Algorithms for Highly Efficient, Load-Balanced, and Scalable Molecular Simulation.
2008,
Pubmed
Heyers,
A visual pathway links brain structures active during magnetic compass orientation in migratory birds.
2007,
Pubmed
Hiscock,
The quantum needle of the avian magnetic compass.
2016,
Pubmed
Hong,
Photoactivation of cryptochromes from Drosophila melanogaster and Sylvia borin: insight into the chemical compass mechanism by computational investigation.
2015,
Pubmed
Hore,
The Radical-Pair Mechanism of Magnetoreception.
2016,
Pubmed
Hornak,
Comparison of multiple Amber force fields and development of improved protein backbone parameters.
2006,
Pubmed
Humphrey,
VMD: visual molecular dynamics.
1996,
Pubmed
Immeln,
Primary events in the blue light sensor plant cryptochrome: intraprotein electron and proton transfer revealed by femtosecond spectroscopy.
2012,
Pubmed
Johnsen,
The physics and neurobiology of magnetoreception.
2005,
Pubmed
Kubar,
What governs the charge transfer in DNA? The role of DNA conformation and environment.
2008,
Pubmed
Kubar,
Coarse-grained time-dependent density functional simulation of charge transfer in complex systems: application to hole transfer in DNA.
2010,
Pubmed
Kubař,
Efficient algorithms for the simulation of non-adiabatic electron transfer in complex molecular systems: application to DNA.
2013,
Pubmed
Kubař,
A hybrid approach to simulation of electron transfer in complex molecular systems.
2013,
Pubmed
Langenbacher,
Microsecond light-induced proton transfer to flavin in the blue light sensor plant cryptochrome.
2009,
Pubmed
Liedvogel,
Chemical magnetoreception: bird cryptochrome 1a is excited by blue light and forms long-lived radical-pairs.
2007,
Pubmed
Liedvogel,
Cryptochromes--a potential magnetoreceptor: what do we know and what do we want to know?
2010,
Pubmed
Lohmann,
The neurobiology of magnetoreception in vertebrate animals.
2000,
Pubmed
Lüdemann,
Solvent driving force ensures fast formation of a persistent and well-separated radical pair in plant cryptochrome.
2015,
Pubmed
Lüdemann,
Charge transfer in E. coli DNA photolyase: understanding polarization and stabilization effects via QM/MM simulations.
2013,
Pubmed
Mouritsen,
Cryptochromes and neuronal-activity markers colocalize in the retina of migratory birds during magnetic orientation.
2004,
Pubmed
Mouritsen,
Magnetoreception and its use in bird navigation.
2005,
Pubmed
Müller,
Discovery and functional analysis of a 4th electron-transferring tryptophan conserved exclusively in animal cryptochromes and (6-4) photolyases.
2015,
Pubmed
Nielsen,
Ascorbic acid may not be involved in cryptochrome-based magnetoreception.
2017,
Pubmed
Niessner,
Avian ultraviolet/violet cones identified as probable magnetoreceptors.
2011,
Pubmed
Nohr,
Extended Electron-Transfer in Animal Cryptochromes Mediated by a Tetrad of Aromatic Amino Acids.
2016,
Pubmed
Pedersen,
Multiscale description of avian migration: from chemical compass to behaviour modeling.
2016,
Pubmed
Phillips,
Wavelength-dependent effects of light on magnetic compass orientation in Drosophila melanogaster.
1993,
Pubmed
Pronk,
GROMACS 4.5: a high-throughput and highly parallel open source molecular simulation toolkit.
2013,
Pubmed
Ritz,
A model for photoreceptor-based magnetoreception in birds.
2000,
Pubmed
Rodgers,
Chemical magnetoreception in birds: the radical pair mechanism.
2009,
Pubmed
Sancar,
Regulation of the mammalian circadian clock by cryptochrome.
2004,
Pubmed
Sjulstok,
Quantifying electron transfer reactions in biological systems: what interactions play the major role?
2015,
Pubmed
Solov'yov,
Magnetic field effects in Arabidopsis thaliana cryptochrome-1.
2007,
Pubmed
Solov'yov,
Acuity of a cryptochrome and vision-based magnetoreception system in birds.
2010,
Pubmed
Solov'yov,
Decrypting cryptochrome: revealing the molecular identity of the photoactivation reaction.
2012,
Pubmed
Solov'yov,
Separation of photo-induced radical pair in cryptochrome to a functionally critical distance.
2014,
Pubmed
Wang,
Development and testing of a general amber force field.
2004,
Pubmed
Wiltschko,
Light-dependent magnetoreception in birds: the effect of intensity of 565-nm green light.
2000,
Pubmed
Woiczikowski,
Nonadiabatic QM/MM simulations of fast charge transfer in Escherichia coli DNA photolyase.
2011,
Pubmed
Zapka,
Visual but not trigeminal mediation of magnetic compass information in a migratory bird.
2009,
Pubmed
Zoltowski,
Structure of full-length Drosophila cryptochrome.
2011,
Pubmed
