Lorena Harris H and Zalik SE (1982), The presence of an endogenous lectin in early e...

XB-ART-54509

Wilehm Roux Arch Dev Biol 1982 May 01;1913:208-210. doi: 10.1007/BF00848338.

Show Gene links Show Anatomy links

The presence of an endogenous lectin in early embryos ofXenopus laevis.

Lorena Harris H , Zalik SE .

???displayArticle.abstract???
Xenopus laevis embryos were examined for the presence of endogenous carbohydrate binding proteins. Soluble extracts of cleavage, gastrula and neurula embryos are able to agglutinate trypsinized rabbit erythrocytes. Unlike other embryonic lectins this agglutination activity requires the presence of calcium ions but not of sulphydryl reducing agents. It is specifically inhibited by galactose and galactose containing derivatives. Thiodigalactoside is the most potent disaccharide inhibitor followed by lactose and melibiose respectively. Methyl α-D-galactopyranoside is a more effective inhibitor than its β anomer. N-acetyl-D-galactosamine, N-acetyl-D-glucosamine, methyl α-D-mannopyranoside andL-fucose do not inhibit activity at concentrations at or above 25 mM. EDTA and EGTA are also strong inhibitors of this activity. β-D-galactoside binding lectins present in the early chick embryo have been implicated in cell to cell and cell to substrate adhesiveness of extraembryonic chick endoderm cells. Since cells of the blastula inXenopus laevis possess surface receptors bearing terminal β-D-galactoside groups it is possible that this β-D-galactoside binding lectin may play a role in the control of cell surface mediated events during development.

???displayArticle.pubmedLink??? 28305386
???displayArticle.link??? Wilehm Roux Arch Dev Biol

References [+] :

Barondes, Lectins: their multiple endogenous cellular functions. 1981, Pubmed

Barondes, Lectins: their multiple endogenous cellular functions. 1981, Pubmed
Bradford, A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. 1976, Pubmed
Cook, A lectin which binds specifically to beta-D-galactoside groups is present at the earliest stages of chick embryo development. 1979, Pubmed
Den, Isolation and properties of beta-D-galactoside-specific lectin from chick embryo thigh muscle. 1977, Pubmed
Fraser, Lectin-mediated agglutination of amphibian embryonic cells. 1977, Pubmed , Xenbase
Kitamura, The changes in lectin activity during the development of embryonic chick skin. 1980, Pubmed
Kobiler, Lectin activity from embryonic chick brain, heart, and liver: changes with development. 1977, Pubmed
Milos, Mechanisms of adhesion among cells of the early chick blastoderm: role of the beta-D-galactoside-binding lectin in the adhesion of extraembryonic endoderm cells. 1982, Pubmed
Milos, Effect of theβ-D-galactoside-binding lectin on cell to substratum and cell to cell adhesion of cells from the extraembryonic endoderm of the early chick blastoderm. 1981, Pubmed
Nowak, Developmentally regulated lectin from embryonic chick pectoral muscle. Purification by affinity chromatography. 1977, Pubmed
Podleski, Studies on lectin activity during myogenesis. 1979, Pubmed
Rosen, Developmentally regulated, carbohydrate-binding protein in Dictyostelium discoideum. 1973, Pubmed
Wyrick, Agglutination of jelly coat and cortical granule components and the block to polyspermy in the amphibian Xenopus laevis. 1974, Pubmed , Xenbase