Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Bio Protoc
2017 Apr 20;78:. doi: 10.21769/BioProtoc.2224.
Show Gene links
Show Anatomy links
Expression and Analysis of Flow-regulated Ion Channels in Xenopus Oocytes.
Shi S
,
Carattino MD
.
???displayArticle.abstract???
Mechanically-gated ion channels play key roles in mechanotransduction, a process that translates physical forces into biological signals. Epithelial and endothelial cells are exposed to laminar shear stress (LSS), a tangential force exerted by flowing fluids against the wall of vessels and epithelia. The protocol outlined herein has been used to examine the response of ion channels expressed in Xenopus oocytes to LSS (Hoger et al., 2002; Carattino et al., 2004; Shi et al., 2006). The Xenopus oocyte is a reliable system that allows for the expression and chemical modification of ion channels and regulatory proteins (George et al., 1989; Palmer et al., 1990; Sheng et al., 2001; Carattino et al., 2003). Therefore, this technique is suitable for studying the molecular mechanisms that allow flow-activated channels to respond to LSS.
Abi-Antoun,
Second transmembrane domain modulates epithelial sodium channel gating in response to shear stress.
2011, Pubmed,
Xenbase
Abi-Antoun,
Second transmembrane domain modulates epithelial sodium channel gating in response to shear stress.
2011,
Pubmed
,
Xenbase
Althaus,
Mechano-sensitivity of epithelial sodium channels (ENaCs): laminar shear stress increases ion channel open probability.
2007,
Pubmed
,
Xenbase
Carattino,
Arachidonic acid regulates surface expression of epithelial sodium channels.
2003,
Pubmed
,
Xenbase
Carattino,
Epithelial Na+ channels are activated by laminar shear stress.
2004,
Pubmed
,
Xenbase
Carattino,
Mutations in the pore region modify epithelial sodium channel gating by shear stress.
2005,
Pubmed
,
Xenbase
Carattino,
Lack of a role of membrane-protein interactions in flow-dependent activation of ENaC.
2007,
Pubmed
,
Xenbase
Davies,
Flow-mediated endothelial mechanotransduction.
1995,
Pubmed
Driscoll,
The mec-4 gene is a member of a family of Caenorhabditis elegans genes that can mutate to induce neuronal degeneration.
1991,
Pubmed
Fronius,
Epithelial Na+ channels derived from human lung are activated by shear force.
2010,
Pubmed
,
Xenbase
George,
Functional expression of the amiloride-sensitive sodium channel in Xenopus oocytes.
1989,
Pubmed
,
Xenbase
Guo,
Role of epithelial Na+ channels in endothelial function.
2016,
Pubmed
Hoger,
Shear stress regulates the endothelial Kir2.1 ion channel.
2002,
Pubmed
,
Xenbase
Liu,
Effect of flow and stretch on the [Ca2+]i response of principal and intercalated cells in cortical collecting duct.
2003,
Pubmed
Morimoto,
Mechanism underlying flow stimulation of sodium absorption in the mammalian collecting duct.
2006,
Pubmed
,
Xenbase
Palmer,
Expression of epithelial Na channels in Xenopus oocytes.
1990,
Pubmed
,
Xenbase
Ranade,
Mechanically Activated Ion Channels.
2015,
Pubmed
Satlin,
Epithelial Na(+) channels are regulated by flow.
2001,
Pubmed
,
Xenbase
Satlin,
Regulation of cation transport in the distal nephron by mechanical forces.
2006,
Pubmed
Sheng,
Epithelial sodium channel pore region. structure and role in gating.
2001,
Pubmed
,
Xenbase
Shi,
Role of the wrist domain in the response of the epithelial sodium channel to external stimuli.
2012,
Pubmed
,
Xenbase
Shi,
Activation of the Caenorhabditis elegans Degenerin Channel by Shear Stress Requires the MEC-10 Subunit.
2016,
Pubmed
,
Xenbase
Shi,
ENaC regulation by proteases and shear stress.
2013,
Pubmed
Shi,
Extracellular finger domain modulates the response of the epithelial sodium channel to shear stress.
2012,
Pubmed
,
Xenbase
Shi,
Base of the thumb domain modulates epithelial sodium channel gating.
2011,
Pubmed
,
Xenbase
Wang,
Functional ENaC channels expressed in endothelial cells: a new candidate for mediating shear force.
2009,
Pubmed
Weinbaum,
Mechanotransduction in the renal tubule.
2010,
Pubmed
Woda,
Flow-dependent K+ secretion in the cortical collecting duct is mediated by a maxi-K channel.
2001,
Pubmed