Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Am J Physiol Renal Physiol
2016 Dec 01;3116:F1280-F1293. doi: 10.1152/ajprenal.00556.2015.
Show Gene links
Show Anatomy links
Structural determinants of NH3 and NH4+ transport by mouse Rhbg, a renal Rh glycoprotein.
Abdulnour-Nakhoul S
,
Le T
,
Rabon E
,
Hamm LL
,
Nakhoul NL
.
???displayArticle.abstract???
Renal Rhbg is localized to the basolateral membrane of intercalated cells and is involved in NH3/NH4+ transport. The structure of Rhbg is not yet resolved; however, a high-resolution crystal structure of AmtB, a bacterial homolog of Rh, has been determined. We aligned the sequence of Rhbg to that of AmtB and identified important sites of Rhbg that may affect transport. Our analysis positioned three conserved amino acids, histidine 183 (H183), histidine 342 (H342), and tryptophan 230 (W230), within the hydrophobic pore where they presumably serve to control NH3 transport. A fourth residue, phenylalanine 128 (F128) was positioned at the upper vestibule, presumably contributing to recruitment of NH4+ We generated three mutations each of H183, H342, W230, and F128 and expressed them in frog oocytes. Immunolabeling showed that W230 and F128 mutants were localized to the cell membrane, whereas H183 and H342 staining was diffuse and mostly intracellular. To determine function, we compared measurements of NH3/NH4+ and methyl amine (MA)/methyl ammonium (MA+)-induced currents, intracellular pH, and surface pH (pHs) among oocytes expressing the mutants, Rhbg, or injected with H2O. In H183 and W230 mutants, NH4+-induced current and intracellular acidification were inhibited compared with that of Rhbg, and MA-induced intracellular alkalinization was completely absent. Expression of H183A or W230A mutants inhibited NH3/NH4+- and MA/MA+-induced decrease in pHs to the level observed in H2O-injected oocytes. Mutations of F128 did not significantly affect transport of NH3 or NH4+ These data demonstrated that mutating H183 or W230 caused loss of function but not F128. H183 and H342 may affect membrane expression of the transporter.
Ammann,
Neutral carrier based hydrogen ion selective microelectrode for extra- and intracellular studies.
1981, Pubmed
Ammann,
Neutral carrier based hydrogen ion selective microelectrode for extra- and intracellular studies.
1981,
Pubmed
Attmane-Elakeb,
Ammonium carriers in medullary thick ascending limb.
2001,
Pubmed
Bakouh,
NH3 is involved in the NH4+ transport induced by the functional expression of the human Rh C glycoprotein.
2004,
Pubmed
,
Xenbase
Bishop,
Role of the Rhesus glycoprotein, Rh B glycoprotein, in renal ammonia excretion.
2010,
Pubmed
Biver,
A role for Rhesus factor Rhcg in renal ammonium excretion and male fertility.
2008,
Pubmed
Callebaut,
Hydrophobic cluster analysis and modeling of the human Rh protein three-dimensional structures.
2006,
Pubmed
Callebaut,
Hydrophobic cluster analysis reveals a third chromodomain in the Tetrahymena Pdd1p protein of the chromo superfamily.
1997,
Pubmed
Caner,
Mechanisms of ammonia and ammonium transport by rhesus-associated glycoproteins.
2015,
Pubmed
,
Xenbase
Conroy,
Modelling the human rhesus proteins: implications for structure and function.
2005,
Pubmed
Good,
Ammonia and bicarbonate transport by thick ascending limb of rat kidney.
1984,
Pubmed
Good,
Ammonia absorption by the thick ascending limb of Henle's loop.
1985,
Pubmed
Gruswitz,
Function of human Rh based on structure of RhCG at 2.1 A.
2010,
Pubmed
Hamm,
Ammonium chloride inhibits Na+ and K+ transport in the cortical collecting tubule.
1985,
Pubmed
Han,
Expression of rh glycoproteins in the Mammalian kidney.
2009,
Pubmed
Handlogten,
Expression of the ammonia transporter proteins Rh B glycoprotein and Rh C glycoprotein in the intestinal tract.
2005,
Pubmed
Honnappa,
Key interaction modes of dynamic +TIP networks.
2006,
Pubmed
Javelle,
Substrate binding, deprotonation, and selectivity at the periplasmic entrance of the Escherichia coli ammonia channel AmtB.
2008,
Pubmed
Javelle,
Structural and mechanistic aspects of Amt/Rh proteins.
2007,
Pubmed
Khademi,
Mechanism of ammonia transport by Amt/MEP/Rh: structure of AmtB at 1.35 A.
2004,
Pubmed
Kim,
Natural history of transposition in the green alga Chlamydomonas reinhardtii: use of the AMT4 locus as an experimental system.
2006,
Pubmed
Knepper,
Ammonium transport in the kidney.
1989,
Pubmed
Knepper,
Regulation of renal medullary ammonium accumulation.
1991,
Pubmed
Lee,
Expression of the rhesus glycoproteins, ammonia transporter family members, RHCG and RHBG in male reproductive organs.
2013,
Pubmed
Li,
Structural and functional insights into the AmtB/Mep/Rh protein family.
2006,
Pubmed
Liu,
Characterization of human RhCG and mouse Rhcg as novel nonerythroid Rh glycoprotein homologues predominantly expressed in kidney and testis.
2000,
Pubmed
Liu,
Rh type B glycoprotein is a new member of the Rh superfamily and a putative ammonia transporter in mammals.
2001,
Pubmed
Ludewig,
Electroneutral ammonium transport by basolateral rhesus B glycoprotein.
2004,
Pubmed
,
Xenbase
Mak,
Characterization of ammonia transport by the kidney Rh glycoproteins RhBG and RhCG.
2006,
Pubmed
,
Xenbase
Marini,
The Rh (rhesus) blood group polypeptides are related to NH4+ transporters.
1997,
Pubmed
Marini,
The human Rhesus-associated RhAG protein and a kidney homologue promote ammonium transport in yeast.
2000,
Pubmed
Nakhoul,
Characteristics of mammalian Rh glycoproteins (SLC42 transporters) and their role in acid-base transport.
2013,
Pubmed
Nakhoul,
Electrogenic ammonium transport by renal Rhbg.
2006,
Pubmed
,
Xenbase
Nakhoul,
Substrate specificity of Rhbg: ammonium and methyl ammonium transport.
2010,
Pubmed
Nakhoul,
Transport of NH(3)/NH in oocytes expressing aquaporin-1.
2001,
Pubmed
,
Xenbase
Nakhoul,
pH sensitivity of ammonium transport by Rhbg.
2010,
Pubmed
,
Xenbase
Nakhoul,
Characteristics of renal Rhbg as an NH4(+) transporter.
2005,
Pubmed
,
Xenbase
Pieren,
Crystal structure of the Orf virus NZ2 variant of vascular endothelial growth factor-E. Implications for receptor specificity.
2006,
Pubmed
Quentin,
RhBG and RhCG, the putative ammonia transporters, are expressed in the same cells in the distal nephron.
2003,
Pubmed
Van Kim,
Rh proteins: key structural and functional components of the red cell membrane.
2006,
Pubmed
Verlander,
Localization of the ammonium transporter proteins RhBG and RhCG in mouse kidney.
2003,
Pubmed
Wang,
Ammonium transporters achieve charge transfer by fragmenting their substrate.
2012,
Pubmed
Weiner,
Ammonia transport in the kidney by Rhesus glycoproteins.
2014,
Pubmed
Weiner,
Localization of the ammonium transporters, Rh B glycoprotein and Rh C glycoprotein, in the mouse liver.
2003,
Pubmed
Winkler,
Amt/MEP/Rh proteins conduct ammonia.
2006,
Pubmed
Zheng,
The mechanism of ammonia transport based on the crystal structure of AmtB of Escherichia coli.
2004,
Pubmed
Zidi-Yahiaoui,
Human Rhesus B and Rhesus C glycoproteins: properties of facilitated ammonium transport in recombinant kidney cells.
2005,
Pubmed
Zidi-Yahiaoui,
Ammonium transport properties of HEK293 cells expressing RhCG mutants: preliminary analysis of structure/function by site-directed mutagenesis.
2006,
Pubmed
