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Rouxs Arch Dev Biol
1986 Feb 01;1952:117-122. doi: 10.1007/BF00456107.
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Isolation of plasma membranes from Xenopus embryos.
Bretzel G
,
Janeczek J
,
Born J
,
John M
,
Tiedemann H
,
Tiedemann H
.
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Plasma membranes were isolated in high yield from Xenopus gastrulae by repeated sedimentation in discontinuous sucrose gradients. Most of the yolk was separated by lowspeed sedimentation before centrifugation on the discontinuous sucrose gradients. The isolation of plasma membranes was followed by covalent labelling of the surface of dissociated gastrula cells with diazoniobenzene sulphonate, by electron microscopy and the distribution of enzymatic markers. The isolated plasma membranes have a low neural inducing activity as compared to other cell constituents.
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Berg,
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Born,
Biological activity of the vegetalizing factor: Decrease after coupling to polysaccharide matrix and enzymatic recovery of active factor.
1980,
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Edelman,
Cell adhesion molecules.
1983,
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Emmelot,
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1966,
Pubmed
Grunz,
A new method for the isolation of plasma membranes from amphibian embryos.
1980,
Pubmed
Grunz,
Effect of concanavalin A and vegetalizing factor on the outer and inner ectoderm layers of early gastrulae of Xenopus laevis after treatment with cytochalasin B.
1985,
Pubmed
,
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Janeczek,
Inducing activity of subcellular fractions from amphibian embryos.
1984,
Pubmed
,
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Neuhoff,
A simple, versatile, sensitive and volume-independent method for quantitative protein determination which is independent of other external influences.
1979,
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Ray,
A modified method for the isolation of the plasma membrane from rat liver.
1970,
Pubmed
Reinwald,
Diazoniobenzenesulfonate as marker for cell surface proteins: study of the surface coat of Trypanosoma congolense.
1978,
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Ribot,
Preparation of plasma membranes from fertilized sea urchin eggs.
1983,
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Takata,
Use of lectins as probes for analyzing embryonic induction.
1981,
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Tiedemann,
Biological activity of vegetalizing and neuralizing inducing factors after binding to BAC-cellulose and CNBr-sepharose.
1978,
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