Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Rouxs Arch Dev Biol
1990 Nov 01;1993:174-180. doi: 10.1007/BF01681491.
Show Gene links
Show Anatomy links
Temporally uncontrolled expression of linearized plasmid DNA which carries bacterial chloramphenicol acetyltransferase gene withXenopus cardiacα-actin promoter after injection intoXenopus fertilized eggs.
Shiokawa K
,
Fu Y
,
Hosokawa K
,
Yamana K
.
???displayArticle.abstract???
Circular and linearized plasmid DNA which contained bacterial chloramphenicol acetyltransferase (CAT) gene connected toXenopus cardiacα-actin promoter was injected intoXenopus fertilized eggs to study their expression in the course of early embryonic development. While circular DNA was slightly replicated and expressed only after embryos reached neurula stage, linearized DNA formed a large amount of concatemers, and was expressed as early as at blastula stage, or about 14 hr earlier than the time of circular DNA expression. Similarly earlier expression of linearized DNA occurred slightly more strongly when the DNA was injected into presumptive dorsal than in ventral blastomeres at 4-cell stage, and the expression was not affected when embryos were dissociated at blastula stage and their cells were cultured under reaggregating or nonreaggregating conditions. These results show that although circular actin-CAT fusion gene is expressed during development according to endogenous temporal control, the expression of linearized DNA deviates from such developmental control even though it contains intact promoter ofα-actin gene. It is then recommended that study of the control of the expression of exogenously-introduced DNA inXenopus fertilized eggs should be carried out with circular but not linearized plasmids.
Andres,
Persistence, methylation and expression of vitellogenin gene derivatives after injection into fertilized eggs of Xenopus laevis.
1984, Pubmed,
Xenbase
Andres,
Persistence, methylation and expression of vitellogenin gene derivatives after injection into fertilized eggs of Xenopus laevis.
1984,
Pubmed
,
Xenbase
Atsuchi,
Level of histone H4 mRNA in Xenopus laevis embryonic cells cultured in the absence of cell adhesion.
1986,
Pubmed
,
Xenbase
Bendig,
Persistence and expression of histone genes injected into Xenopus eggs in early development.
1981,
Pubmed
,
Xenbase
Bendig,
Differential expression of the Xenopus laevis tadpole and adult beta-globin genes when injected into fertilized Xenopus laevis eggs.
1984,
Pubmed
,
Xenbase
Bendig,
Replication and expression of Xenopus laevis globin genes injected into fertilized Xenopus eggs.
1983,
Pubmed
,
Xenbase
Busby,
Spacer sequences regulate transcription of ribosomal gene plasmids injected into Xenopus embryos.
1983,
Pubmed
,
Xenbase
Etkin,
Transformed Xenopus embryos as a transient expression system to analyze gene expression at the midblastula transition.
1985,
Pubmed
,
Xenbase
Etkin,
Replication, integration and expression of exogenous DNA injected into fertilized eggs of Xenopus laevis.
1984,
Pubmed
,
Xenbase
Etkin,
Distribution, expression and germ line transmission of exogenous DNA sequences following microinjection into Xenopus laevis eggs.
1987,
Pubmed
,
Xenbase
Fu,
Expression of circular and linearized bacterial chloramphenicol acetyltransferase genes with or without viral promoters after injection into fertilized eggs, unfertilized eggs and oocytes ofXenopus laevis.
1989,
Pubmed
,
Xenbase
Giebelhaus,
Antisense RNA inhibits expression of membrane skeleton protein 4.1 during embryonic development of Xenopus.
1988,
Pubmed
,
Xenbase
Gorman,
Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.
1982,
Pubmed
Gurdon,
Embryonic induction--molecular prospects.
1987,
Pubmed
Gurdon,
Transcription of muscle-specific actin genes in early Xenopus development: nuclear transplantation and cell dissociation.
1984,
Pubmed
,
Xenbase
Gurdon,
All components required for the eventual activation of muscle-specific actin genes are localized in the subequatorial region of an uncleaved amphibian egg.
1985,
Pubmed
,
Xenbase
Krieg,
Developmental regulation of a gastrula-specific gene injected into fertilized Xenopus eggs.
1985,
Pubmed
,
Xenbase
Krieg,
An enhancer responsible for activating transcription at the midblastula transition in Xenopus development.
1987,
Pubmed
,
Xenbase
Langner,
Trans effect of the E1 region of adenoviruses on the expression of a prokaryotic gene in mammalian cells: resistance to 5' -CCGG- 3' methylation.
1986,
Pubmed
,
Xenbase
Marini,
Persistence and replication of plasmid DNA microinjected into early embryos of Xenopus laevis.
1988,
Pubmed
,
Xenbase
Melton,
Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.
1984,
Pubmed
Miyahara,
Cellular commitment for post-gastrular increase in alkaline phosphatase activity in Xenopus laevis development.
1982,
Pubmed
,
Xenbase
Mohun,
Upstream sequences required for tissue-specific activation of the cardiac actin gene in Xenopus laevis embryos.
1986,
Pubmed
,
Xenbase
Mohun,
Cell type-specific activation of actin genes in the early amphibian embryo.
,
Pubmed
,
Xenbase
Newport,
A major developmental transition in early Xenopus embryos: II. Control of the onset of transcription.
1982,
Pubmed
,
Xenbase
Rusconi,
Transformation of frog embryos with a rabbit beta-globin gene.
1981,
Pubmed
,
Xenbase
Shiokawa,
Persistence and expression of circular DNAs encoding Drosophila amylase, bacterial chloramphenicol acetyltransferase, and others in Xenopus laevis embryos.
1989,
Pubmed
,
Xenbase
Shiokawa,
Electron microscopic studies of giant nucleus-like structure formed by lambda DNA introduced into the cytoplasm of Xenopus laevis fertilized eggs and embryos.
1987,
Pubmed
,
Xenbase
Shiokawa,
Formation of nucleus-like structure in the cytoplasm of lambda-DNA-injected fertilized eggs and its partition into blastomeres during early embryogenesis in Xenopus laevis.
1986,
Pubmed
,
Xenbase
Shiokawa,
Pattern of RNA synthesis in isolated cells of Xenopus laevis embryos.
1967,
Pubmed
,
Xenbase
Shiokawa,
Expression of exogenously introduced bacterial chloramphenicol acetyltransferase genes in Xenopus laevis embryos before the midblastula transition.
1990,
Pubmed
,
Xenbase
Shiokawa,
Effects of the injection of exogenous DNAs on gene expression in early embryos and coenocytic egg cells ofXenopus laevis.
1989,
Pubmed
,
Xenbase
Steinbeisser,
Different regulatory elements are required for cell-type and stage specific expression of the Xenopus laevis skeletal muscle actin gene upon injection in X.laevis oocytes and embryos.
1988,
Pubmed
,
Xenbase
Tashiro,
Preservation of Xenopus laevis rDNA-containing plasmid, pXlr101A, injected into the fertilized egg of Xenopus laevis.
1986,
Pubmed
,
Xenbase
Tashiro,
Determination of the rate of rRNA synthesis in Xenopus laevis triploid embryos produced by low-temperature treatment.
1983,
Pubmed
,
Xenbase
Tashiro,
Structural analysis of ribosomal DNA homologues in nucleolus-less mutant of Xenopus laevis.
1986,
Pubmed
,
Xenbase
Wilson,
Tissue-specific expression of actin genes injected into Xenopus embryos.
1986,
Pubmed
,
Xenbase