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Rouxs Arch Dev Biol
1992 Feb 01;2011:30-35. doi: 10.1007/BF00188773.
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Partial characterization of neural-inducing factors from Xenopus gastrulae Evidence for a larger protein complex containing the factor.
Janeczek J
,
Born J
,
Hoppe P
,
Tiedemann H
.
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High (Mr ≈ 90-110 kDa) and low (Mr ≈ 15-30 kDa) molecular weight forms of neural-inducing factors have been found in the supernatant of Xenopus gastrula homogenate. The factors, which are protein in nature, have been partially purified by size exclusion high-performance liquid chromatography (HPLC) and sodium dodecyl sulphate (SDS)-polyacrylamide gel electrophoresis. The factor of smaller size, which could be derived from a precursor, is associated with other proteins in a larger complex. The neural-inducing factors are not irreversibly inactivated after chemical deglycosylation with trifluoromethansulfonic acid. The neural-inducing protein which is found in ribonucleoprotein (RNP)-particles was partially purified by hydrophobic chromatography. Possible relationships of the factors in different subcellular fractions and their physiological significance are discussed.
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