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Rouxs Arch Dev Biol
1992 Oct 01;2016:340-345. doi: 10.1007/BF00365121.
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An expression vector inhibits gene expression in Xenopus embryos by antisense RNA.
Schmid M
,
Steinbeisser H
,
Epperlein HH
,
Trendelenburg MF
,
Lipps HJ
.
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An expression vector was constructed containing the entire bovine papilloma virus (BPV-1) genome and part of the a-actin gene of Xenopus laevis cloned in the antisense orientation into the neomycin resistance gene under the control of the herpes simplex virus (HSV) thymidine kinase (TK) promoter. When this vector is microinjected into X. laevis embryos it replicates extrachromosomally, at least up to the tadpole stage, and a fusion RNA is synthesized after the mid blastula transition (MBT). The expression of the antisense gene results in a morphological abnormality of somites demonstrating that antisense RNA generated by an episomal replicating expression vector can inhibit the expression of a selected gene during early embryogenesis of X. laevis.
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Bass,
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,
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Budd,
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Crowley,
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1985,
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Feinberg,
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1983,
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Gehring,
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1990,
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Giebelhaus,
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1988,
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,
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Holt,
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1986,
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Izant,
Inhibition of thymidine kinase gene expression by anti-sense RNA: a molecular approach to genetic analysis.
1984,
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Kiełbówna,
The formation of somites and early myotomal myogenesis in Xenopus laevis, Bombina variegata and Pelobates fuscus.
1981,
Pubmed
,
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Kim,
Stable reduction of thymidine kinase activity in cells expressing high levels of anti-sense RNA.
1985,
Pubmed
Kloc,
The maternal store of the xlgv7 mRNA in full-grown oocytes is not required for normal development in Xenopus.
1989,
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,
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Matthias,
A bovine papilloma virus vector with a dominant resistance marker replicates extrachromosomally in mouse and E. coli cells.
1983,
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Melton,
Injected anti-sense RNAs specifically block messenger RNA translation in vivo.
1985,
Pubmed
,
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Minshull,
Translation of cyclin mRNA is necessary for extracts of activated xenopus eggs to enter mitosis.
1989,
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,
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Rebagliati,
Antisense RNA injections in fertilized frog eggs reveal an RNA duplex unwinding activity.
1987,
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,
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Rosenberg,
Production of phenocopies by Krüppel antisense RNA injection into Drosophila embryos.
,
Pubmed
Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed
Schmid,
A bovine papillomavirus type-1 (BPV-1) containing plasmid replicates extrachromosomally in Xenopus embryos.
1990,
Pubmed
,
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Steinbeisser,
Different regulatory elements are required for cell-type and stage specific expression of the Xenopus laevis skeletal muscle actin gene upon injection in X.laevis oocytes and embryos.
1988,
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,
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Stutz,
Isolation and characterization of sarcomeric actin genes expressed in Xenopus laevis embryos.
1986,
Pubmed
,
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Weintraub,
Expression of transfected DNA depends on DNA topology.
1986,
Pubmed
Wormington,
Stable repression of ribosomal protein L1 synthesis in Xenopus oocytes by microinjection of antisense RNA.
1986,
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,
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