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Nucleic Acids Res
2000 Oct 15;2820:3880-6. doi: 10.1093/nar/28.20.3880.
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Mitochondrial DNA ligase III function is independent of Xrcc1.
Lakshmipathy U
,
Campbell C
.
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Hamster EM9 cells, which lack Xrcc1 protein, have reduced levels of DNA ligase III and are defective in nuclear base excision repair. The Xrcc1 protein stabilizes DNA ligase III and may even play a direct role in catalyzing base excision repair. Since DNA ligase III is also thought to function in mitochondrial base excision repair, it seemed likely that mitochondrial DNA ligase III function would also be dependent upon Xrcc1. However, several lines of evidence indicate that this is not the case. First, western blot analysis failed to detect Xrcc1 protein in mitochondrial extracts. Second, DNA ligase III levels present in mitochondrial protein extracts from EM9 cells were indistinguishable from those seen in similar extracts from wild-type (AA8) cells. Third, the mitochondrial DNA content of both cell lines was identical. Fourth, EM9 cells displayed no defect in their ability to repair spontaneous mitochondrial DNA damage. Fifth, while EM9 cells were far more sensitive to the cytotoxic effects of ionizing radiation due to a defect in nuclear DNA repair, there was no apparent difference in the ability of EM9 and AA8 cells to restore their mitochondrial DNA to pre-irradiation levels. Thus, mitochondrial DNA ligase III function is independent of the Xrcc1 protein.
Abraham,
Modified diphenylamine reaction for increased sensitivity.
1972, Pubmed
Abraham,
Modified diphenylamine reaction for increased sensitivity.
1972,
Pubmed
Barker,
An improved assay for DNA ligase reveals temperature-sensitive activity in cdc9 mutants of Saccharomyces cerevisiae.
1985,
Pubmed
Bradford,
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
1976,
Pubmed
Bryans,
Absence of DNA ligase IV protein in XR-1 cells: evidence for stabilization by XRCC4.
1999,
Pubmed
Caldecott,
XRCC1 polypeptide interacts with DNA polymerase beta and possibly poly (ADP-ribose) polymerase, and DNA ligase III is a novel molecular 'nick-sensor' in vitro.
1996,
Pubmed
,
Xenbase
Caldecott,
An interaction between the mammalian DNA repair protein XRCC1 and DNA ligase III.
1994,
Pubmed
,
Xenbase
Caldecott,
Characterization of the XRCC1-DNA ligase III complex in vitro and its absence from mutant hamster cells.
1995,
Pubmed
,
Xenbase
Cappelli,
Involvement of XRCC1 and DNA ligase III gene products in DNA base excision repair.
1997,
Pubmed
,
Xenbase
Chew,
An exonic splicing silencer in the testes-specific DNA ligase III beta exon.
2000,
Pubmed
,
Xenbase
Critchlow,
Mammalian DNA double-strand break repair protein XRCC4 interacts with DNA ligase IV.
1997,
Pubmed
Grawunder,
Activity of DNA ligase IV stimulated by complex formation with XRCC4 protein in mammalian cells.
1997,
Pubmed
Jessberger,
Repair of deletions and double-strand gaps by homologous recombination in a mammalian in vitro system.
1991,
Pubmed
Kubota,
Reconstitution of DNA base excision-repair with purified human proteins: interaction between DNA polymerase beta and the XRCC1 protein.
1996,
Pubmed
Lakshmipathy,
The human DNA ligase III gene encodes nuclear and mitochondrial proteins.
1999,
Pubmed
,
Xenbase
Ljungquist,
Altered DNA ligase III activity in the CHO EM9 mutant.
1994,
Pubmed
,
Xenbase
Marintchev,
Solution structure of the single-strand break repair protein XRCC1 N-terminal domain.
1999,
Pubmed
Masson,
XRCC1 is specifically associated with poly(ADP-ribose) polymerase and negatively regulates its activity following DNA damage.
1998,
Pubmed
,
Xenbase
Pinz,
Efficient repair of abasic sites in DNA by mitochondrial enzymes.
1998,
Pubmed
,
Xenbase
Shen,
Mutations in hamster single-strand break repair gene XRCC1 causing defective DNA repair.
1998,
Pubmed
Storrie,
Isolation of subcellular organelles.
1990,
Pubmed
Thompson,
XRCC1 keeps DNA from getting stranded.
2000,
Pubmed
Thompson,
Validation of conditions for efficient detection of HPRT and APRT mutations in suspension-cultured Chinese hamster ovary cells.
1980,
Pubmed
Thompson,
A CHO-cell strain having hypersensitivity to mutagens, a defect in DNA strand-break repair, and an extraordinary baseline frequency of sister-chromatid exchange.
1982,
Pubmed
Thompson,
Molecular cloning of the human XRCC1 gene, which corrects defective DNA strand break repair and sister chromatid exchange.
1990,
Pubmed
Tomkinson,
Three distinct DNA ligases in mammalian cells.
1991,
Pubmed
,
Xenbase
