Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Base excision repair is limited by different proteins in male germ cell nuclear extracts prepared from young and old mice.
Intano GW
,
McMahan CA
,
McCarrey JR
,
Walter RB
,
McKenna AE
,
Matsumoto Y
,
MacInnes MA
,
Chen DJ
,
Walter CA
.
???displayArticle.abstract???
The combined observations of elevated DNA repair gene expression, high uracil-DNA glycosylase-initiated base excision repair, and a low spontaneous mutant frequency for a lacI transgene in spermatogenic cells from young mice suggest that base excision repair activity is high in spermatogenic cell types. Notably, the spontaneous mutant frequency of the lacI transgene is greater in spermatogenic cells obtained from old mice, suggesting that germ line DNA repair activity may decline with age. A paternal age effect in spermatogenic cells is recognized for the human population as well. To determine if male germ cell base excision repair activity changes with age, uracil-DNA glycosylase-initiated base excision repair activity was measured in mixed germ cell (i.e., all spermatogenic cell types in adult testis) nuclear extracts prepared from young, middle-aged, and old mice. Base excision repair activity was also assessed in nuclear extracts from premeiotic, meiotic, and postmeiotic spermatogenic cell types obtained from young mice. Mixed germ cell nuclear extracts exhibited an age-related decrease in base excision repair activity that was restored by addition of apurinic/apyrimidinic (AP) endonuclease. Uracil-DNA glycosylase and DNA ligase were determined to be limiting in mixed germ cell nuclear extracts prepared from young animals. Base excision repair activity was only modestly elevated in pachytene spermatocytes and round spermatids relative to other spermatogenic cells. Thus, germ line short-patch base excision repair activity appears to be relatively constant throughout spermatogenesis in young animals, limited by uracil-DNA glycosylase and DNA ligase in young animals, and limited by AP endonuclease in old animals.
Alcivar,
DNA polymerase-beta and poly(ADP)ribose polymerase mRNAs are differentially expressed during the development of male germinal cells.
1992, Pubmed
Alcivar,
DNA polymerase-beta and poly(ADP)ribose polymerase mRNAs are differentially expressed during the development of male germinal cells.
1992,
Pubmed
Beard,
Structural design of a eukaryotic DNA repair polymerase: DNA polymerase beta.
2000,
Pubmed
Bellvé,
Spermatogenic cells of the prepuberal mouse. Isolation and morphological characterization.
1977,
Pubmed
Bellvé,
Dissociation of the mouse testis and characterization of isolated spermatogenic cells.
1977,
Pubmed
Bellvé,
Purification, culture, and fractionation of spermatogenic cells.
1993,
Pubmed
Biade,
Impairment of proliferating cell nuclear antigen-dependent apurinic/apyrimidinic site repair on linear DNA.
1998,
Pubmed
Bradford,
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
1976,
Pubmed
Caldecott,
An interaction between the mammalian DNA repair protein XRCC1 and DNA ligase III.
1994,
Pubmed
,
Xenbase
Caldecott,
Characterization of the XRCC1-DNA ligase III complex in vitro and its absence from mutant hamster cells.
1995,
Pubmed
,
Xenbase
Cappelli,
Involvement of XRCC1 and DNA ligase III gene products in DNA base excision repair.
1997,
Pubmed
,
Xenbase
Chen,
Mammalian DNA ligase III: molecular cloning, chromosomal localization, and expression in spermatocytes undergoing meiotic recombination.
1995,
Pubmed
,
Xenbase
Crow,
The mutation component of genetic damage.
1981,
Pubmed
Crow,
The high spontaneous mutation rate: is it a health risk?
1997,
Pubmed
Dycaico,
The use of shuttle vectors for mutation analysis in transgenic mice and rats.
1994,
Pubmed
Engelward,
Cloning and characterization of a mouse 3-methyladenine/7-methyl-guanine/3-methylguanine DNA glycosylase cDNA whose gene maps to chromosome 11.
1993,
Pubmed
Erickson,
A study of parental age effects on the occurrence of fresh mutations for the Apert syndrome.
1974,
Pubmed
Favor,
Multiple endpoint mutational analysis in the mouse.
1990,
Pubmed
Frosina,
Two pathways for base excision repair in mammalian cells.
1996,
Pubmed
Glaser,
Paternal origin of FGFR2 mutations in sporadic cases of Crouzon syndrome and Pfeiffer syndrome.
2000,
Pubmed
Glassner,
The influence of DNA glycosylases on spontaneous mutation.
1998,
Pubmed
Gold,
Haploid accumulation and translational control of phosphoglycerate kinase-2 messenger RNA during mouse spermatogenesis.
1983,
Pubmed
Griffin,
Non-disjunction in human sperm: evidence for an effect of increasing paternal age.
1995,
Pubmed
Hirose,
Difference in the expression level of DNA polymerase beta among mouse tissues: high expression in the pachytene spermatocyte.
1989,
Pubmed
Intano,
Mixed spermatogenic germ cell nuclear extracts exhibit high base excision repair activity.
2001,
Pubmed
Izumi,
Requirement for human AP endonuclease 1 for repair of 3'-blocking damage at DNA single-strand breaks induced by reactive oxygen species.
2000,
Pubmed
Jansen,
Nucleotide excision repair in rat male germ cells: low level of repair in intact cells contrasts with high dual incision activity in vitro.
2001,
Pubmed
Kleene,
Translational regulation and deadenylation of a protamine mRNA during spermiogenesis in the mouse.
1984,
Pubmed
Kleene,
Patterns of translational regulation in the mammalian testis.
1996,
Pubmed
Klungland,
Second pathway for completion of human DNA base excision-repair: reconstitution with purified proteins and requirement for DNase IV (FEN1).
1997,
Pubmed
Kohler,
Spectra of spontaneous and mutagen-induced mutations in the lacI gene in transgenic mice.
1991,
Pubmed
Lindahl,
DNA excision repair pathways.
1997,
Pubmed
Lowe,
Aneuploidies and micronuclei in the germ cells of male mice of advanced age.
1995,
Pubmed
Ludwig,
A murine AP-endonuclease gene-targeted deficiency with post-implantation embryonic progression and ionizing radiation sensitivity.
1998,
Pubmed
Mackey,
An alternative splicing event which occurs in mouse pachytene spermatocytes generates a form of DNA ligase III with distinct biochemical properties that may function in meiotic recombination.
1997,
Pubmed
,
Xenbase
Matsui,
Testis- and developmental stage-specific expression of hnRNP A2/B1 splicing isoforms, B0a/b.
2000,
Pubmed
Matsumoto,
Base excision repair assay using Xenopus laevis oocyte extracts.
1999,
Pubmed
,
Xenbase
Matsumoto,
Proliferating cell nuclear antigen-dependent abasic site repair in Xenopus laevis oocytes: an alternative pathway of base excision DNA repair.
1994,
Pubmed
,
Xenbase
Matsumoto,
Haploid specific activations of protamine 1 and hsc70t genes in mouse spermatogenesis.
1993,
Pubmed
McInnes,
Donor age and the frequency of disomy for chromosomes 1, 13, 21 and structural abnormalities in human spermatozoa using multicolour fluorescence in-situ hybridization.
1998,
Pubmed
Meira,
Heterozygosity for the mouse Apex gene results in phenotypes associated with oxidative stress.
2001,
Pubmed
Morales,
Cytoplasmic localization during storage and translation of the mRNAs of transition protein 1 and protamine 1, two translationally regulated transcripts of the mammalian testis.
1991,
Pubmed
Murdoch,
Parental age effects on the occurrence of new mutations for the Marfan syndrome.
1972,
Pubmed
Müller,
Radiation-induced malformations after exposure of murine germ cells in various stages of spermatogenesis.
1999,
Pubmed
Nelson,
Malformations due to presumed spontaneous mutations in newborn infants.
1989,
Pubmed
Nomura,
X-ray- and chemically induced germ-line mutation causing phenotypical anomalies in mice.
1988,
Pubmed
Olsen,
Highly efficient base excision repair (BER) in human and rat male germ cells.
2001,
Pubmed
Olshan,
Paternal age and the risk of congenital heart defects.
1994,
Pubmed
Ramana,
Activation of apurinic/apyrimidinic endonuclease in human cells by reactive oxygen species and its correlation with their adaptive response to genotoxicity of free radicals.
1998,
Pubmed
Romrell,
Separation of mouse spermatogenic cells by sedimentation velocity. A morphological characterization.
1976,
Pubmed
Russell,
Unlike other chemicals, etoposide (a topoisomerase-II inhibitor) produces peak mutagenicity in primary spermatocytes of the mouse.
1998,
Pubmed
Shelby,
Fertility, reproduction, and genetic disease: studies on the mutagenic effects of environmental agents on mammalian germ cells.
1993,
Pubmed
Shi,
Aneuploidy in human sperm: a review of the frequency and distribution of aneuploidy, effects of donor age and lifestyle factors.
2000,
Pubmed
Singhal,
DNA polymerase beta conducts the gap-filling step in uracil-initiated base excision repair in a bovine testis nuclear extract.
1995,
Pubmed
Sobol,
Requirement of mammalian DNA polymerase-beta in base-excision repair.
1996,
Pubmed
Srivastava,
Mammalian abasic site base excision repair. Identification of the reaction sequence and rate-determining steps.
1998,
Pubmed
Stern,
Gene expression during mammalian spermatogenesis. III. Changes in populations of mRNA during spermiogenesis.
1983,
Pubmed
Walter,
Xrcc-1 expression during male meiosis in the mouse.
1996,
Pubmed
Walter,
Mutation frequency declines during spermatogenesis in young mice but increases in old mice.
1998,
Pubmed
Walter,
Testis and somatic Xrcc-1 DNA repair gene expression.
1994,
Pubmed
Wei,
Molecular cloning and expression of human cDNAs encoding a novel DNA ligase IV and DNA ligase III, an enzyme active in DNA repair and recombination.
1995,
Pubmed
,
Xenbase
Widen,
Mammalian beta-polymerase promoter: large-scale purification and properties of ATF/CREB palindrome binding protein from bovine testes.
1991,
Pubmed
Wilson,
Differential expression of the apurinic / apyrimidinic endonuclease (APE/ref-1) multifunctional DNA base excision repair gene during fetal development and in adult rat brain and testis.
1996,
Pubmed
Winn,
Detection of mutations in transgenic fish carrying a bacteriophage lambda cII transgene target.
2000,
Pubmed
Zhou,
Expression of the DNA repair gene XRCC1 in baboon tissues.
1995,
Pubmed
de Wet,
Cloning of firefly luciferase cDNA and the expression of active luciferase in Escherichia coli.
1985,
Pubmed
de Wet,
Firefly luciferase gene: structure and expression in mammalian cells.
1987,
Pubmed