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Stimulation of NEIL2-mediated oxidized base excision repair via YB-1 interaction during oxidative stress.
Das S
,
Chattopadhyay R
,
Bhakat KK
,
Boldogh I
,
Kohno K
,
Prasad R
,
Wilson SH
,
Hazra TK
.
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The recently characterized enzyme NEIL2 (Nei-like-2), one of the four oxidized base-specific DNA glycosylases (OGG1, NTH1, NEIL1, and NEIL2) in mammalian cells, has poor base excision activity from duplex DNA. To test the possibility that one or more proteins modulate its activity in vivo, we performed mass spectrometric analysis of the NEIL2 immunocomplex and identified Y box-binding (YB-1) protein as a stably interacting partner of NEIL2. We show here that YB-1 not only interacts physically with NEIL2, but it also cooperates functionally by stimulating its base excision activity by 7-fold. Moreover, YB-1 interacts with the other NEIL2-associated BER proteins, namely, DNA ligase III alpha and DNA polymerase beta and thus could form a large multiprotein complex. YB-1, normally present in the cytoplasm, translocates to the nucleus during UVA-induced oxidative stress, concomitant with its increased association with and activation of NEIL2. NEIL2-initiated base excision activity is significantly reduced in YB-1-depleted cells. YB-1 thus appears to have a novel regulatory role in NEIL2-mediated repair under oxidative stress.
Ames,
Oxidants, antioxidants, and the degenerative diseases of aging.
1993, Pubmed
Ames,
Oxidants, antioxidants, and the degenerative diseases of aging.
1993,
Pubmed
Bandaru,
A novel human DNA glycosylase that removes oxidative DNA damage and is homologous to Escherichia coli endonuclease VIII.
2002,
Pubmed
Bargou,
Nuclear localization and increased levels of transcription factor YB-1 in primary human breast cancers are associated with intrinsic MDR1 gene expression.
1997,
Pubmed
Bhakat,
Acetylation of human 8-oxoguanine-DNA glycosylase by p300 and its role in 8-oxoguanine repair in vivo.
2006,
Pubmed
Boldogh,
ROS generated by pollen NADPH oxidase provide a signal that augments antigen-induced allergic airway inflammation.
2005,
Pubmed
Campalans,
UVA irradiation induces relocalisation of the DNA repair protein hOGG1 to nuclear speckles.
2007,
Pubmed
Das,
Induction of the human oxidized base-specific DNA glycosylase NEIL1 by reactive oxygen species.
2005,
Pubmed
Das,
NEIL2-initiated, APE-independent repair of oxidized bases in DNA: Evidence for a repair complex in human cells.
2006,
Pubmed
,
Xenbase
Dianova,
Interaction of human AP endonuclease 1 with flap endonuclease 1 and proliferating cell nuclear antigen involved in long-patch base excision repair.
2001,
Pubmed
Didier,
Characterization of the cDNA encoding a protein binding to the major histocompatibility complex class II Y box.
1988,
Pubmed
Dou,
Repair of oxidized bases in DNA bubble structures by human DNA glycosylases NEIL1 and NEIL2.
2003,
Pubmed
Gaudreault,
YB-1 promotes strand separation in vitro of duplex DNA containing either mispaired bases or cisplatin modifications, exhibits endonucleolytic activities and binds several DNA repair proteins.
2004,
Pubmed
Graumann,
A superfamily of proteins that contain the cold-shock domain.
1998,
Pubmed
,
Xenbase
Götz,
Oxidative stress: free radical production in neural degeneration.
1994,
Pubmed
Hasegawa,
DNA binding properties of YB-1 and dbpA: binding to double-stranded, single-stranded, and abasic site containing DNAs.
1991,
Pubmed
Hayakawa,
Binding capacity of human YB-1 protein for RNA containing 8-oxoguanine.
2002,
Pubmed
Hazra,
Identification and characterization of a human DNA glycosylase for repair of modified bases in oxidatively damaged DNA.
2002,
Pubmed
Hazra,
Identification and characterization of a novel human DNA glycosylase for repair of cytosine-derived lesions.
2002,
Pubmed
Higashi,
Interferon-gamma interferes with transforming growth factor-beta signaling through direct interaction of YB-1 with Smad3.
2003,
Pubmed
Ikeda,
Purification and characterization of human NTH1, a homolog of Escherichia coli endonuclease III. Direct identification of Lys-212 as the active nucleophilic residue.
1998,
Pubmed
Ise,
Transcription factor Y-box binding protein 1 binds preferentially to cisplatin-modified DNA and interacts with proliferating cell nuclear antigen.
1999,
Pubmed
Jayaraman,
High mobility group protein-1 (HMG-1) is a unique activator of p53.
1998,
Pubmed
Kappes,
No major role for 7,8-dihydro-8-oxoguanine in ultraviolet light-induced mutagenesis.
2005,
Pubmed
Kasai,
Formation of 8-hydroxyguanine moiety in cellular DNA by agents producing oxygen radicals and evidence for its repair.
1986,
Pubmed
Klungland,
Accumulation of premutagenic DNA lesions in mice defective in removal of oxidative base damage.
1999,
Pubmed
Kohno,
The pleiotropic functions of the Y-box-binding protein, YB-1.
2003,
Pubmed
Koike,
Nuclear translocation of the Y-box binding protein by ultraviolet irradiation.
1997,
Pubmed
Kozmin,
UVA radiation is highly mutagenic in cells that are unable to repair 7,8-dihydro-8-oxoguanine in Saccharomyces cerevisiae.
2005,
Pubmed
Kvam,
The role of melanin in the induction of oxidative DNA base damage by ultraviolet A irradiation of DNA or melanoma cells.
1999,
Pubmed
Lowe,
Low doses of repetitive ultraviolet A induce morphologic changes in human skin.
1995,
Pubmed
Lu,
A mammalian DNA repair enzyme that excises oxidatively damaged guanines maps to a locus frequently lost in lung cancer.
1997,
Pubmed
Marenstein,
Stimulation of human endonuclease III by Y box-binding protein 1 (DNA-binding protein B). Interaction between a base excision repair enzyme and a transcription factor.
2001,
Pubmed
Minowa,
Mmh/Ogg1 gene inactivation results in accumulation of 8-hydroxyguanine in mice.
2000,
Pubmed
Mitra,
Complexities of DNA base excision repair in mammalian cells.
1997,
Pubmed
Mitra,
Choreography of oxidative damage repair in mammalian genomes.
2002,
Pubmed
Morland,
Human DNA glycosylases of the bacterial Fpg/MutM superfamily: an alternative pathway for the repair of 8-oxoguanine and other oxidation products in DNA.
2002,
Pubmed
Ocampo,
Targeted deletion of mNth1 reveals a novel DNA repair enzyme activity.
2002,
Pubmed
Oda,
Nuclear expression of YB-1 protein correlates with P-glycoprotein expression in human osteosarcoma.
1998,
Pubmed
Ogawa,
Radiation-induced reactive oxygen species formation prior to oxidative DNA damage in human peripheral T cells.
2003,
Pubmed
Ohga,
Role of the human Y box-binding protein YB-1 in cellular sensitivity to the DNA-damaging agents cisplatin, mitomycin C, and ultraviolet light.
1996,
Pubmed
Parrish,
Erythema and melanogenesis action spectra of normal human skin.
1982,
Pubmed
Petersen,
Hydrogen peroxide is responsible for UVA-induced DNA damage measured by alkaline comet assay in HaCaT keratinocytes.
2000,
Pubmed
Pouget,
DNA damage induced in cells by gamma and UVA radiation as measured by HPLC/GC-MS and HPLC-EC and Comet assay.
2000,
Pubmed
Rosenquist,
The novel DNA glycosylase, NEIL1, protects mammalian cells from radiation-mediated cell death.
2003,
Pubmed
Sarasin,
The molecular pathways of ultraviolet-induced carcinogenesis.
1999,
Pubmed
Schreiber,
Rapid detection of octamer binding proteins with 'mini-extracts', prepared from a small number of cells.
1989,
Pubmed
Sommerville,
Masking of mRNA by Y-box proteins.
1996,
Pubmed
Sommerville,
Transcription and masking of mRNA in germ cells: involvement of Y-box proteins.
1996,
Pubmed
,
Xenbase
Stein,
Hyperthermia-induced nuclear translocation of transcription factor YB-1 leads to enhanced expression of multidrug resistance-related ABC transporters.
2001,
Pubmed
Takao,
A back-up glycosylase in Nth1 knock-out mice is a functional Nei (endonuclease VIII) homologue.
2002,
Pubmed
Vidal,
XRCC1 coordinates the initial and late stages of DNA abasic site repair through protein-protein interactions.
2001,
Pubmed
Wagner,
Endogenous oxidative damage of deoxycytidine in DNA.
1992,
Pubmed
Wang,
Quantifying cellular oxidative stress by dichlorofluorescein assay using microplate reader.
1999,
Pubmed
Wang,
Ultraviolet A and melanoma: a review.
2001,
Pubmed
Wiederhold,
AP endonuclease-independent DNA base excision repair in human cells.
2004,
Pubmed
,
Xenbase
Wolffe,
Structural and functional properties of the evolutionarily ancient Y-box family of nucleic acid binding proteins.
1994,
Pubmed