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Mol Cell Biol
2003 Jun 01;2311:3763-73. doi: 10.1128/MCB.23.11.3763-3773.2003.
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Progesterone and glucocorticoid receptors recruit distinct coactivator complexes and promote distinct patterns of local chromatin modification.
Li X
,
Wong J
,
Tsai SY
,
Tsai MJ
,
O'Malley BW
.
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It is well established that steroid receptor function requires interaction with coactivators. However, the mechanisms through which steroid receptors elicit precise assembly of coactivator complexes and the way the steroid activation signal is transduced remain elusive. Using a T47D cell line stably integrated with a mouse mammary tumor virus-chloramphenicol acetyltransferase (MMTV-CAT) reporter, we demonstrate that specific steroid receptors exhibit preferential recruitment of SRC-1 family coactivators, which determines the subsequent recruitment of specific downstream coregulator molecules. Upon ligand treatment, progesterone receptor (PR) interacted preferentially with SRC-1, which recruited CBP and significantly enhanced acetylation at K5 of histone H4. In contrast, activated glucocorticoid receptor (GR) preferentially associated with SRC-2 (TIF-2/GRIP-1), which subsequently recruited pCAF and led to specific modification of histone H3, suggesting that specific coactivators recruit distinct histone acetyltransferases to modulate the transcription of steroid-responsive genes. Loss-of-function experiments further support the predicted roles of SRC-1 and SRC-2 in, respectively, PR- and GR-mediated transcription on the MMTV promoter. This study indicates that differential recruitment of coactivators by nuclear receptors determines the assembly of coactivator complexes on target promoters to mediate specific transcription signals.
Alen,
Interaction of the putative androgen receptor-specific coactivator ARA70/ELE1alpha with multiple steroid receptors and identification of an internally deleted ELE1beta isoform.
1999, Pubmed
Alen,
Interaction of the putative androgen receptor-specific coactivator ARA70/ELE1alpha with multiple steroid receptors and identification of an internally deleted ELE1beta isoform.
1999,
Pubmed
Allan,
Synergism between steroid response and promoter elements during cell-free transcription.
1991,
Pubmed
Bhakat,
Regulation of the human O(6)-methylguanine-DNA methyltransferase gene by transcriptional coactivators cAMP response element-binding protein-binding protein and p300.
2000,
Pubmed
Boyd,
Coexamination of site-specific transcription factor binding and promoter activity in living cells.
1999,
Pubmed
Bramlett,
Effects of selective estrogen receptor modulators (SERMs) on coactivator nuclear receptor (NR) box binding to estrogen receptors.
2002,
Pubmed
Børud,
The nuclear receptor coactivators p300/CBP/cointegrator-associated protein (p/CIP) and transcription intermediary factor 2 (TIF2) differentially regulate PKA-stimulated transcriptional activity of steroidogenic factor 1.
2002,
Pubmed
Chen,
Synergistic, p160 coactivator-dependent enhancement of estrogen receptor function by CARM1 and p300.
2000,
Pubmed
Chen,
HATs on and beyond chromatin.
2001,
Pubmed
Darimont,
Structure and specificity of nuclear receptor-coactivator interactions.
1998,
Pubmed
Deroo,
Glucocorticoid receptor-mediated chromatin remodeling in vivo.
2001,
Pubmed
Ding,
Nuclear receptor-binding sites of coactivators glucocorticoid receptor interacting protein 1 (GRIP1) and steroid receptor coactivator 1 (SRC-1): multiple motifs with different binding specificities.
1998,
Pubmed
Elbashir,
Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells.
2001,
Pubmed
Gehin,
The function of TIF2/GRIP1 in mouse reproduction is distinct from those of SRC-1 and p/CIP.
2002,
Pubmed
He,
Modulation of induction properties of glucocorticoid receptor-agonist and -antagonist complexes by coactivators involves binding to receptors but is independent of ability of coactivators to augment transactivation.
2002,
Pubmed
Heery,
A signature motif in transcriptional co-activators mediates binding to nuclear receptors.
1997,
Pubmed
Hong,
GRIP1, a transcriptional coactivator for the AF-2 transactivation domain of steroid, thyroid, retinoid, and vitamin D receptors.
1997,
Pubmed
Jenuwein,
Translating the histone code.
2001,
Pubmed
Kalkhoven,
Isoforms of steroid receptor co-activator 1 differ in their ability to potentiate transcription by the oestrogen receptor.
1998,
Pubmed
Kamei,
A CBP integrator complex mediates transcriptional activation and AP-1 inhibition by nuclear receptors.
1996,
Pubmed
Kimura,
How do histone acetyltransferases select lysine residues in core histones?
1998,
Pubmed
Korzus,
Transcription factor-specific requirements for coactivators and their acetyltransferase functions.
1998,
Pubmed
Kuo,
Transcription-linked acetylation by Gcn5p of histones H3 and H4 at specific lysines.
1996,
Pubmed
Kusk,
Characterization of an NF-1/CTF family member as a functional activator of the mouse mammary tumor virus long terminal repeat 5' enhancer.
1996,
Pubmed
Le Bihan,
Calcium/calmodulin kinase inhibitors and immunosuppressant macrolides rapamycin and FK506 inhibit progestin- and glucocorticosteroid receptor-mediated transcription in human breast cancer T47D cells.
1998,
Pubmed
Leers,
Mechanistic principles in NR box-dependent interaction between nuclear hormone receptors and the coactivator TIF2.
1998,
Pubmed
Lefstin,
Allosteric effects of DNA on transcriptional regulators.
1998,
Pubmed
Leo,
Differential mechanisms of nuclear receptor regulation by receptor-associated coactivator 3.
2000,
Pubmed
Li,
p300 requires its histone acetyltransferase activity and SRC-1 interaction domain to facilitate thyroid hormone receptor activation in chromatin.
2000,
Pubmed
,
Xenbase
Liu,
Sequential recruitment of steroid receptor coactivator-1 (SRC-1) and p300 enhances progesterone receptor-dependent initiation and reinitiation of transcription from chromatin.
2001,
Pubmed
Lo,
Phosphorylation of serine 10 in histone H3 is functionally linked in vitro and in vivo to Gcn5-mediated acetylation at lysine 14.
2000,
Pubmed
McInerney,
Determinants of coactivator LXXLL motif specificity in nuclear receptor transcriptional activation.
1998,
Pubmed
McKenna,
Distinct steady-state nuclear receptor coregulator complexes exist in vivo.
1998,
Pubmed
McKenna,
Nuclear receptor coregulators: cellular and molecular biology.
1999,
Pubmed
Mukherjee,
Ligand and coactivator recruitment preferences of peroxisome proliferator activated receptor alpha.
2002,
Pubmed
Nolte,
Ligand binding and co-activator assembly of the peroxisome proliferator-activated receptor-gamma.
1998,
Pubmed
Oñate,
Sequence and characterization of a coactivator for the steroid hormone receptor superfamily.
1995,
Pubmed
Rosenfeld,
Coregulator codes of transcriptional regulation by nuclear receptors.
2001,
Pubmed
Sadow,
Specificity of thyroid hormone receptor subtype and steroid receptor coactivator-1 on thyroid hormone action.
2003,
Pubmed
Schiltz,
Overlapping but distinct patterns of histone acetylation by the human coactivators p300 and PCAF within nucleosomal substrates.
1999,
Pubmed
Shang,
Cofactor dynamics and sufficiency in estrogen receptor-regulated transcription.
2000,
Pubmed
Shang,
Molecular determinants for the tissue specificity of SERMs.
2002,
Pubmed
Sharma,
Ordered recruitment of histone acetyltransferases and the TRAP/Mediator complex to thyroid hormone-responsive promoters in vivo.
2002,
Pubmed
Sheppard,
Analysis of the steroid receptor coactivator 1 (SRC1)-CREB binding protein interaction interface and its importance for the function of SRC1.
2001,
Pubmed
Smith,
CREB binding protein acts synergistically with steroid receptor coactivator-1 to enhance steroid receptor-dependent transcription.
1996,
Pubmed
Smith,
Differential activity of progesterone and glucocorticoid receptors on mouse mammary tumor virus templates differing in chromatin structure.
1997,
Pubmed
Torchia,
The transcriptional co-activator p/CIP binds CBP and mediates nuclear-receptor function.
1997,
Pubmed
Treuter,
Competition between thyroid hormone receptor-associated protein (TRAP) 220 and transcriptional intermediary factor (TIF) 2 for binding to nuclear receptors. Implications for the recruitment of TRAP and p160 coactivator complexes.
1999,
Pubmed
Voegel,
The coactivator TIF2 contains three nuclear receptor-binding motifs and mediates transactivation through CBP binding-dependent and -independent pathways.
1998,
Pubmed
Xu,
E1A-mediated repression of progesterone receptor-dependent transactivation involves inhibition of the assembly of a multisubunit coactivation complex.
2000,
Pubmed
Xu,
Partial hormone resistance in mice with disruption of the steroid receptor coactivator-1 (SRC-1) gene.
1998,
Pubmed
Yi,
The effects of estrogen-responsive element- and ligand-induced structural changes on the recruitment of cofactors and transcriptional responses by ER alpha and ER beta.
2002,
Pubmed