XB-ART-52394
Food Chem
2017 Jan 01;214:354-359. doi: 10.1016/j.foodchem.2016.07.048.
Show Gene links
Show Anatomy links
Direct activation of GABAA receptors by substances in the organic acid fraction of Japanese sake.
???displayArticle.abstract???
We investigated the effect of substances present in Japanese sake on the response of ionotropic γ-aminobutyric acid (GABA)A receptors expressed in Xenopus oocytes. Sake was fractionated by ion-exchange chromatography. The fraction containing organic acids (OA fraction) showed agonist activities on the GABAA receptor. OA fractions from sake were analyzed by capillary electrophoresis time-of-flight mass spectrometry (CE-TOFMS). Of the 64 compounds identified, 13 compounds showed GABAA receptor agonist activities. Especially, l-lactic acid showed high agonist activity and its EC50 value was 37μM. Intraperitoneal injections of l-lactic acid, gluconic acid, and pyruvic acid (10, 10, and 5mg/kg BW, respectively), which showed agonistic activity on the GABAA receptor, led to significant anxiolytic effects during an elevated plus-maze test in mice.
???displayArticle.pubmedLink??? 27507485
???displayArticle.link??? Food Chem
Species referenced: Xenopus laevis
Genes referenced: gabarap