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ERYTHROPOIESIS DURING AMPHIBIAN METAMORPHOSIS : III. Immunochemical Detection of Tadpole and Frog Hemoglobins (Rana catesbeiana) in Single Erythrocytes.
Maniatis GM
,
Ingram VM
.
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Rabbit antibodies specific for the major tadpole and frog hemoglobin components of R. catesbeiana were used for the detection of the two hemoglobins inside single cells. The antisera, after fractionation by ammonium sulfate precipitation and diethylaminoethyl (DEAE)-cellulose chromatography, were conjugated with fluorescein isothiocyanate for the antifrog hemoglobin serum and tetramethylrhodamine isothiocyanate for the antitadpole hemoglobin serum. The conjugated fractions, refractionated by stepwise elution from a DEAE-cellulose column, were used for the fluorescent staining of blood smears, livertissue imprints, and smears of liver cell suspensions. Both simultaneous and sequential staining with the two fluorescent preparations indicated that larval and adult hemoglobins were not present within the same erythrocyte during metamorphosis. In other experiments, erythroid cells from animals in metamorphosis were spread on agar containing specific antiserum. Precipitates were formed around the cells which contain the particular hemoglobin. The percentages of cells containing either tadpole or frog hemoglobin were estimated within the experimental error of the method. The data showed that the two hemoglobins are in different cells. It is concluded that the hemoglobin change observed during the metamorphosis of R. catesbeiana is due to the appearance of a new population of erythroid cells containing exclusively frog hemoglobin.
Aggarwal,
The hemoglobins of the bullfrog, Rana catesbeiana. I. Purification, amino acid composition, and oxygen equilibria.
1969, Pubmed
Aggarwal,
The hemoglobins of the bullfrog, Rana catesbeiana. I. Purification, amino acid composition, and oxygen equilibria.
1969,
Pubmed
BAGLIONI,
A STUDY OF HEMOGLOBIN DIFFERENTIATION IN RANA CATESBEIANA.
1963,
Pubmed
BETKE,
[Fetal and residual hemoglobin in the erythrocytes and erythroblasts of human fetuses and newborn infants].
1958,
Pubmed
COLBERG,
LOCALIZATION BY IMMUNOFLUORESCENCE OF GAMMA-GLOBULIN ALLOTYPES IN LYMPH NODE CELLS OF HOMOZYGOUS AND HETEROZYGOUS RABBITS.
1964,
Pubmed
CRAIG,
A DEVELOPMENTAL CHANGE IN HEMOGLOBINS CORRELATED WITH AN EMBRYONIC RED CELL POPULATION IN THE MOUSE.
1964,
Pubmed
Cebra,
Chromatographic purification of tetramethylrhodamine-immune globulin conjugates and their use in the cellular localization of rabbit gamma-globulin polypeptide chains.
1965,
Pubmed
D'Amelio,
Differentiation of the cell membrane in embryonic chick blood cells.
1967,
Pubmed
Dan,
Detection of two types of hemoglobin (HbA and HbF) in single erythrocytes by fluorescent antibody technique.
1967,
Pubmed
Day,
Effects of progesterone on fertilization and egg transport in the pig.
1968,
Pubmed
De Witt,
Microcytic response to thyroxine administration.
1968,
Pubmed
FAHEY,
QUANTITATIVE DETERMINATION OF SERUM IMMUNOGLOBULINS IN ANTIBODY-AGAR PLATES.
1965,
Pubmed
FANELLI,
Studies on the structure of hemoglobin. I. Physicochemical properties of human globin.
1958,
Pubmed
Fantoni,
Control mechanisms of the conversion from synthesis of embryonic to adult hemoglobin.
1969,
Pubmed
GOLDSTEIN,
Studies on fluorescent antibody staining. I. Non-specific fluorescence with fluorescein-coupled sheep anti-rabbit globulins.
1961,
Pubmed
Gitlin,
Immunochemical quantitation of proteins in single cells. I. Carbon anhydrase B, beta-chain hemoglobin and gamma-chain hemoglobin in some normal and abnormal erythrocytes.
1968,
Pubmed
Gitlin,
Immunoglobulins G, A, and M determined in single cells from human tonsil.
1969,
Pubmed
Green,
Specificity of the antibodies produced by single cells following immunization with antigens bearing two types of antigenic determinants.
1967,
Pubmed
Hollyfield,
Erythrocyte replacement at metamorphosis in the frog, Rana pipiens.
1966,
Pubmed
Jurd,
An immunofluorescent study of the haemoglobins in metamorphosing Xenopus laevis.
1970,
Pubmed
,
Xenbase
Kagen,
Localization of myoglobin in human skeletal muscle using fluorescent antibody technique.
1967,
Pubmed
Kovach,
Erythroid cell development in fetal mice: ultrastructural characteristics and hemoglobin synthesis.
1967,
Pubmed
MCDEVITT,
PURIFICATION AND ANALYSIS OF FLUORESCEIN-LABELED ANTISERA BY COLUMN CHROMATOGRAPHY.
1963,
Pubmed
MCKINNEY,
FACTORS AFFECTING THE RATE OF REACTION OF FLUORESCEIN ISOTHIOCYANATE WITH SERUM PROTEINS.
1964,
Pubmed
Moss,
The repression and induction by thyroxin of hemoglobin synthesis during amphibian metamorphosis.
1965,
Pubmed
Moss,
Hemoglobin synthesis during amphibian metamorphosis. I. Chemical studies on the hemoglobins from the larval and adult stages of Rana catesbeiana.
1968,
Pubmed
Moss,
Hemoglobin synthesis during amphibian metamorphosis. II. Synthesis of adult hemoglobin following thyroxine administration.
1968,
Pubmed
Rosenberg,
Electrophoretic analysis of hemoglobin and isozymes in individual vertebrate cells.
1970,
Pubmed
STONE,
POSSIBLE SOMATIC CELL MATING IN TWIN CATTLE WITH ERYTHROCYTE MOSAICISM.
1964,
Pubmed
TOMODA,
DEMONSTRATION OF FOETAL ERYTHROCYTE BY IMMUNOFLUORESCENT STAINING.
1964,
Pubmed
Volpe,
Somatic cell mating and segregation in chimeric frogs.
1970,
Pubmed
Wilt,
The control of embryonic hemoglobin synthesis.
1967,
Pubmed
Wood,
Fluorescent antibody staining. 3. Preparation of fluorescein-isothiocyanate-labeled antibodies.
1965,
Pubmed