Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Chem Res Toxicol
2016 May 16;295:924-32. doi: 10.1021/acs.chemrestox.6b00076.
Show Gene links
Show Anatomy links
Perfluoroalkylated Substance Effects in Xenopus laevis A6 Kidney Epithelial Cells Determined by ATR-FTIR Spectroscopy and Chemometric Analysis.
Gorrochategui E
,
Lacorte S
,
Tauler R
,
Martin FL
.
???displayArticle.abstract???
The effects of four perfluoroalkylated substances (PFASs), namely, perfluorobutanesulfonate (PFBS), perfluorooctanoic acid (PFOA), perfluorooctanesulfonate (PFOS), and perfluorononanoic acid (PFNA) were assessed in Xenopus laevis A6 kidney epithelial cells by attenuated total reflection Fourier-transform infrared (ATR-FTIR) spectroscopy and chemometric analysis. Principal component analysis-linear discriminant analysis (PCA-LDA) was used to visualize wavenumber-related alterations and ANOVA-simultaneous component analysis (ASCA) allowed data processing considering the underlying experimental design. Both analyses evidenced a higher impact of low-dose PFAS-treatments (10(-9) M) on A6 cells forming monolayers, while there was a larger influence of high-dose PFAS-treatments (10(-5) M) on A6 cells differentiated into dome structures. The observed dose-response PFAS-induced effects were to some extent related to their cytotoxicity: the EC50-values of most influential PFAS-treatments increased (PFOS < PFNA < PFOA ≪ PFBS), and higher-doses of these chemicals induced a larger impact. Major spectral alterations were mainly attributed to DNA/RNA, secondary protein structure, lipids, and fatty acids. Finally, PFOS and PFOA caused a decrease in A6 cell numbers compared to controls, whereas PFBS and PFNA did not significantly change cell population levels. Overall, this work highlights the ability of PFASs to alter A6 cells, whether forming monolayers or differentiated into dome structures, and the potential of PFOS and PFOA to induce cell death.
Figure 1. Schematic representation
of Xenopus laevis A6
kidney epithelial cells forming a monolayer (A) and a dome (B). Direct
inversion images of Giemsa-stained A6 cells disposed on a monolayer
(C) and forming a dome (D). Phase-contrast images of A6 cells disposed
on a monolayer (E) and forming a dome (F). (Scale bars = 150 μm).
(G) Experimental design for the study of PFAS-induced effects (refer
to section 2.3).
(d = day).
Figure 2. One-D
PCA-LDA score plots showing dose–response effects
of PFBS, PFOA, PFOS, and PFNA in the three experiments. *Most discriminant
PFAS-treatment compared to the control.
Figure 3. ASCA score
plots of the first component for the factors “chemical”
and “dose” of the three experiments. *Most discriminant
factor levels.
Figure 4. Effects of PFBS, PFNA, PFOS, and PFOA
on dose- and time-related
cell number increases in culture.
Baker,
Using Fourier transform IR spectroscopy to analyze biological materials.
2014, Pubmed
Baker,
Using Fourier transform IR spectroscopy to analyze biological materials.
2014,
Pubmed
Bird,
Infrared spectral histopathology (SHP): a novel diagnostic tool for the accurate classification of lung cancer.
2012,
Pubmed
Campos,
Identification of metabolic pathways in Daphnia magna explaining hormetic effects of selective serotonin reuptake inhibitors and 4-nonylphenol using transcriptomic and phenotypic responses.
2013,
Pubmed
Cui,
Investigation of the Effects of Perfluorooctanoic Acid (PFOA) and Perfluorooctane Sulfonate (PFOS) on Apoptosis and Cell Cycle in a Zebrafish (Danio rerio) Liver Cell Line.
2015,
Pubmed
Du,
Endocrine-related effects of perfluorooctanoic acid (PFOA) in zebrafish, H295R steroidogenesis and receptor reporter gene assays.
2013,
Pubmed
Gajjar,
Fourier-transform infrared spectroscopy coupled with a classification machine for the analysis of blood plasma or serum: a novel diagnostic approach for ovarian cancer.
2013,
Pubmed
Gorrochategui,
Perfluorinated chemicals: differential toxicity, inhibition of aromatase activity and alteration of cellular lipids in human placental cells.
2014,
Pubmed
Gorrochategui,
Characterization of complex lipid mixtures in contaminant exposed JEG-3 cells using liquid chromatography and high-resolution mass spectrometry.
2014,
Pubmed
Gorrochategui,
Chemometric strategy for untargeted lipidomics: biomarker detection and identification in stressed human placental cells.
2015,
Pubmed
Griffiths,
Mass spectrometry: from proteomics to metabolomics and lipidomics.
2009,
Pubmed
Han,
Renal elimination of perfluorocarboxylates (PFCAs).
2012,
Pubmed
Hands,
Investigating the rapid diagnosis of gliomas from serum samples using infrared spectroscopy and cytokine and angiogenesis factors.
2013,
Pubmed
Hines,
Phenotypic dichotomy following developmental exposure to perfluorooctanoic acid (PFOA) in female CD-1 mice: Low doses induce elevated serum leptin and insulin, and overweight in mid-life.
2009,
Pubmed
Ichigi,
Dome formation and tubule morphogenesis by Xenopus kidney A6 cell cultures exposed to microgravity simulated with a 3D-clinostat and to hypergravity.
2001,
Pubmed
,
Xenbase
Kallenbach-Thieltges,
Immunohistochemistry, histopathology and infrared spectral histopathology of colon cancer tissue sections.
2013,
Pubmed
Kamrin,
The "low dose" hypothesis: validity and implications for human risk.
2007,
Pubmed
Kellenberger,
Epithelial sodium channel/degenerin family of ion channels: a variety of functions for a shared structure.
2002,
Pubmed
Kim,
NMR-based plant metabolomics: where do we stand, where do we go?
2011,
Pubmed
Lawrence,
The effect of short-term low-dose perchlorate on various aspects of thyroid function.
2000,
Pubmed
Li,
Dose-related alterations of carbon nanoparticles in mammalian cells detected using biospectroscopy: potential for real-world effects.
2013,
Pubmed
Llabjani,
Binary mixture effects by PBDE congeners (47, 153, 183, or 209) and PCB congeners (126 or 153) in MCF-7 cells: biochemical alterations assessed by IR spectroscopy and multivariate analysis.
2010,
Pubmed
Llabjani,
Polybrominated diphenyl ether-associated alterations in cell biochemistry as determined by attenuated total reflection Fourier-transform infrared spectroscopy: a comparison with DNA-reactive and/or endocrine-disrupting agents.
2009,
Pubmed
Manzetti,
Chemical properties, environmental fate, and degradation of seven classes of pollutants.
2014,
Pubmed
Martin,
Identifying variables responsible for clustering in discriminant analysis of data from infrared microspectroscopy of a biological sample.
2007,
Pubmed
Martin,
Distinguishing cell types or populations based on the computational analysis of their infrared spectra.
2010,
Pubmed
Ollesch,
FTIR spectroscopy of biofluids revisited: an automated approach to spectral biomarker identification.
2013,
Pubmed
Panaretakis,
Reactive oxygen species and mitochondria mediate the induction of apoptosis in human hepatoma HepG2 cells by the rodent peroxisome proliferator and hepatocarcinogen, perfluorooctanoic acid.
2001,
Pubmed
Pang,
Identification of benzo[a]pyrene-induced cell cycle-associated alterations in MCF-7 cells using infrared spectroscopy with computational analysis.
2012,
Pubmed
Perkins,
Transport properties of toad kidney epithelia in culture.
1981,
Pubmed
,
Xenbase
Rafferty,
The length of secondary chromosomal constrictions in normal individuals and in a nucleolar mutant of Xenopus laevis.
1969,
Pubmed
,
Xenbase
Rehn,
Role of the cytoskeleton in stimulation of Na+ channels in A6 cells by changes in osmolality.
1998,
Pubmed
,
Xenbase
Scaglia,
Noninvasive assessment of hepatic fibrosis in patients with chronic hepatitis C using serum Fourier transform infrared spectroscopy.
2011,
Pubmed
Shabalina,
Effects of the rodent peroxisome proliferator and hepatocarcinogen, perfluorooctanoic acid, on apoptosis in human hepatoma HepG2 cells.
1999,
Pubmed
Smilde,
ANOVA-simultaneous component analysis (ASCA): a new tool for analyzing designed metabolomics data.
2005,
Pubmed
Tanaka,
Proliferation and differentiation of Xenopus A6 cells under hypergravity as revealed by time-lapse imaging.
2003,
Pubmed
,
Xenbase
Theophilou,
A biospectroscopic analysis of human prostate tissue obtained from different time periods points to a trans-generational alteration in spectral phenotype.
2015,
Pubmed
Trevisan,
IRootLab: a free and open-source MATLAB toolbox for vibrational biospectroscopy data analysis.
2013,
Pubmed
Vandenberg,
Hormones and endocrine-disrupting chemicals: low-dose effects and nonmonotonic dose responses.
2012,
Pubmed
Vis,
Statistical validation of megavariate effects in ASCA.
2007,
Pubmed
Walsh,
ATR microspectroscopy with multivariate analysis segregates grades of exfoliative cervical cytology.
2007,
Pubmed
Walsh,
Attenuated total reflectance Fourier-transform infrared spectroscopic imaging for breast histopathology.
2012,
Pubmed
Witorsch,
Low-dose in utero effects of xenoestrogens in mice and their relevance to humans: an analytical review of the literature.
2002,
Pubmed
Yahia,
8-Hydroxy-2'-deoxyguanosine as a biomarker of oxidative DNA damage induced by perfluorinated compounds in TK6 cells.
2016,
Pubmed
vom Saal,
An extensive new literature concerning low-dose effects of bisphenol A shows the need for a new risk assessment.
2005,
Pubmed
