Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Pflugers Arch
2016 May 01;4685:871-80. doi: 10.1007/s00424-016-1800-2.
Show Gene links
Show Anatomy links
CFTR channel in oocytes from Xenopus laevis and its regulation by xShroom1 protein.
Palma AG
,
Galizia L
,
Kotsias BA
,
Marino GI
.
???displayArticle.abstract??? Shroom is a family of related proteins linked to the actin cytoskeleton. xShroom1 is constitutively expressed in Xenopus laevis oocytes, and it is required for the expression of the epithelial sodium channel (ENaC). As there is a close relationship between ENaC and the cystic fibrosis transmembrane regulator (CFTR), we examined the action of xShroom1 on CFTR expression and activity. Biotinylation was used to measure CFTR surface expression, and currents were registered with voltage clamp when stimulated with forskolin and 3-isobutyl-1-methylxanthine. Oocytes were coinjected with CFTR complementary RNAs (cRNAs) and xShroom1 sense or antisense oligonucleotides. We observed an increment in CFTR currents and CFTR surface expression in oocytes coinjected with CFTR and xShroom1 antisense oligonucleotides. MG-132, a proteasome inhibitor, did not prevent the increment in currents when xShroom1 was suppressed by antisense oligonucleotides. In addition, we inhibited the delivery of newly synthesized proteins to the plasma membrane with BFA and we found that the half-life of plasma membraneCFTR was prolonged when coinjected with the xShroom1 antisense oligonucleotides. Chloroquine, an inhibitor of the late endosome/lysosome, did not significantly increase CFTR currents when xShroom1 expression was inhibited. The higher expression of CFTR when xShroom1 is suppressed is in concordance with the functional studies suggesting that the suppression of the xShroom1 protein resulted in an increment in CFTR currents by promoting the increase of the half-life of CFTR in the plasma membrane. The role of xShroom1 in regulating CFTR expression could be relevant in the understanding of the channel malfunction in several diseases.
Ahner,
Cystic fibrosis transmembrane conductance regulator degradation: cross-talk between the ubiquitylation and SUMOylation pathways.
2013, Pubmed
Ahner,
Cystic fibrosis transmembrane conductance regulator degradation: cross-talk between the ubiquitylation and SUMOylation pathways.
2013,
Pubmed
Assef,
ENaC channels in oocytes from Xenopus laevis and their regulation by xShroom1 protein.
2011,
Pubmed
,
Xenbase
Avella,
Characterization of the L683P mutation of SLC26A9 in Xenopus oocytes.
2011,
Pubmed
,
Xenbase
Bear,
Cl- channel activity in Xenopus oocytes expressing the cystic fibrosis gene.
1991,
Pubmed
,
Xenbase
Berdiev,
Molecular proximity of cystic fibrosis transmembrane conductance regulator and epithelial sodium channel assessed by fluorescence resonance energy transfer.
2007,
Pubmed
Bradbury,
Biochemical and biophysical identification of cystic fibrosis transmembrane conductance regulator chloride channels as components of endocytic clathrin-coated vesicles.
1994,
Pubmed
Broadbent,
The cystic fibrosis transmembrane conductance regulator is an extracellular chloride sensor.
2015,
Pubmed
Butterworth,
Acute ENaC stimulation by cAMP in a kidney cell line is mediated by exocytic insertion from a recycling channel pool.
2005,
Pubmed
Cantiello,
Role of actin filament organization in CFTR activation.
2001,
Pubmed
Carattino,
Arachidonic acid regulates surface expression of epithelial sodium channels.
2003,
Pubmed
,
Xenbase
Chardin,
Brefeldin A: the advantage of being uncompetitive.
1999,
Pubmed
Chasan,
Evidence for direct interaction between actin and the cystic fibrosis transmembrane conductance regulator.
2002,
Pubmed
Chen,
Loss of anion transport without increased sodium absorption characterizes newborn porcine cystic fibrosis airway epithelia.
2010,
Pubmed
Cheng,
Ubiquitination and degradation of CFTR by the E3 ubiquitin ligase MARCH2 through its association with adaptor proteins CAL and STX6.
2013,
Pubmed
Collawn,
The CFTR and ENaC debate: how important is ENaC in CF lung disease?
2012,
Pubmed
Drumm,
Chloride conductance expressed by delta F508 and other mutant CFTRs in Xenopus oocytes.
1991,
Pubmed
,
Xenbase
Dye,
hShroom1 links a membrane bound protein to the actin cytoskeleton.
2009,
Pubmed
Edelman,
Cytoskeleton and CFTR.
2014,
Pubmed
Faria,
Regulation of ENaC biogenesis by the stress response protein SERP1.
2012,
Pubmed
,
Xenbase
Gentzsch,
The cystic fibrosis transmembrane conductance regulator impedes proteolytic stimulation of the epithelial Na+ channel.
2010,
Pubmed
Hagens,
A new standard nomenclature for proteins related to Apx and Shroom.
2006,
Pubmed
,
Xenbase
Haigo,
Shroom induces apical constriction and is required for hingepoint formation during neural tube closure.
2003,
Pubmed
,
Xenbase
Hallows,
Physiological modulation of CFTR activity by AMP-activated protein kinase in polarized T84 cells.
2003,
Pubmed
,
Xenbase
Heda,
The Delta F508 mutation shortens the biochemical half-life of plasma membrane CFTR in polarized epithelial cells.
2001,
Pubmed
Hildebrand,
Shroom, a PDZ domain-containing actin-binding protein, is required for neural tube morphogenesis in mice.
1999,
Pubmed
Holleran,
Regulated recycling of mutant CFTR is partially restored by pharmacological treatment.
2013,
Pubmed
Jansen,
Lysosome mediated Kir2.1 breakdown directly influences inward rectifier current density.
2008,
Pubmed
Jensen,
Multiple proteolytic systems, including the proteasome, contribute to CFTR processing.
1995,
Pubmed
Jones,
Role of the NH2 terminus in the assembly and trafficking of the intermediate conductance Ca2+-activated K+ channel hIK1.
2004,
Pubmed
Kunzelmann,
Introduction to section V: assessment of CFTR function.
2011,
Pubmed
,
Xenbase
Mall,
Wild type but not deltaF508 CFTR inhibits Na+ conductance when coexpressed in Xenopus oocytes.
1996,
Pubmed
,
Xenbase
Marino,
Cystic fibrosis transmembrane regulator (CFTR) in human trophoblast BeWo cells and its relation to cell migration.
2014,
Pubmed
Monterisi,
Local modulation of cystic fibrosis conductance regulator: cytoskeleton and compartmentalized cAMP signalling.
2013,
Pubmed
Palma,
[CFTR and ENaC functions in cystic fibrosis].
2014,
Pubmed
Qadri,
CFTR regulation of epithelial sodium channel.
2011,
Pubmed
,
Xenbase
Rotin,
Role of the ubiquitin system in regulating ion transport.
2011,
Pubmed
Staub,
Regulation of stability and function of the epithelial Na+ channel (ENaC) by ubiquitination.
1997,
Pubmed
,
Xenbase
Stutts,
CFTR as a cAMP-dependent regulator of sodium channels.
1995,
Pubmed
Swiatecka-Urban,
The short apical membrane half-life of rescued {Delta}F508-cystic fibrosis transmembrane conductance regulator (CFTR) results from accelerated endocytosis of {Delta}F508-CFTR in polarized human airway epithelial cells.
2005,
Pubmed
Weber,
Functional integrity of the vesicle transporting machinery is required for complete activation of cFTR expressed in xenopus laevis oocytes.
2001,
Pubmed
,
Xenbase
Wilke,
Rescue of murine F508del CFTR activity in native intestine by low temperature and proteasome inhibitors.
2012,
Pubmed
Zuckerman,
Association of the epithelial sodium channel with Apx and alpha-spectrin in A6 renal epithelial cells.
1999,
Pubmed
,
Xenbase