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Evaluation of Presurgical Skin Preparation Agents in African Clawed Frogs (Xenopus laevis).
Philips BH
,
Crim MJ
,
Hankenson FC
,
Steffen EK
,
Klein PS
,
Brice AK
,
Carty AJ
.
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Despite the routine collection of oocytes from African clawed frogs (Xenopus laevis) for use in research, few studies have evaluated methods for preparing their skin for surgery. We evaluated 3 skin preparatory agents by examining their antibacterial efficacy and the gross and microscopic appearance of Xenopus skin after exposure. Frogs (n = 14) were sedated and treated (contact time, 10 min) with 0.9% sterile NaCl on one-half of the ventrum and with 0.5% povidone-iodine or 0.75% chlorhexidine on the other half. Bacterial cultures were obtained before and after skin treatment; bacteria were identified by mass spectrometry. To assess inflammation and degenerative changes, the incision sites were photographed and biopsied at 0, 1, and 7 d after surgery. We isolated at least 22 genera of bacteria from the skin of our frog population (mean ± SE, 5.21 ± 0.82 genera per frog). Iodine (2.00 ± 0.44 genera) and chlorhexidine (0.29 ± 0.76 genera) both had greater antimicrobial activity than did saline. Skin erythema did not correlate with treatment group. Histologic evidence of epidermal degeneration and necrosis was greater on days 1 and 7 after chlorhexidine treatment than after iodine or saline. In addition, frogs treated with chlorhexidine had a higher incidence of clinical illness associated with the exposure site. In summary, although chlorhexidine has adequate antimicrobial activity against organisms on X. laevis skin, it leads to skin damage and subsequent clinical complications. We therefore do not recommend chlorhexidine as a preoperative preparation agent in Xenopus.
Bertolotti,
Skin wound healing in different aged Xenopus laevis.
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Bertolotti,
Skin wound healing in different aged Xenopus laevis.
2013,
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,
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Biswas,
Use of MALDI-TOF mass spectrometry for identification of bacteria that are difficult to culture.
2013,
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Brown,
A tribute to the Xenopus laevis oocyte and egg.
2004,
Pubmed
,
Xenbase
Böhme,
Species differentiation of seafood spoilage and pathogenic gram-negative bacteria by MALDI-TOF mass fingerprinting.
2010,
Pubmed
Böhme,
Rapid species identification of seafood spoilage and pathogenic Gram-positive bacteria by MALDI-TOF mass fingerprinting.
2011,
Pubmed
Chapman,
Safety of chlorhexidine gluconate used for skin antisepsis in the preterm infant.
2012,
Pubmed
Clarke,
The natural history of amphibian skin secretions, their normal functioning and potential medical applications.
1997,
Pubmed
Croxatto,
Applications of MALDI-TOF mass spectrometry in clinical diagnostic microbiology.
2012,
Pubmed
Darouiche,
Chlorhexidine-Alcohol versus Povidone-Iodine for Surgical-Site Antisepsis.
2010,
Pubmed
Del Chierico,
Proteomics boosts translational and clinical microbiology.
2014,
Pubmed
Densmore,
Diseases of amphibians.
2007,
Pubmed
Dieckmann,
Rapid identification and characterization of Vibrio species using whole-cell MALDI-TOF mass spectrometry.
2010,
Pubmed
Elsner,
Poor quality of oocytes from Xenopus laevis used in laboratory experiments: prevention by use of antiseptic surgical technique and antibiotic supplementation.
2000,
Pubmed
,
Xenbase
Franchini,
The thymus and skin wound healing in Xenopus laevis adults.
2014,
Pubmed
,
Xenbase
Gando,
Microvascular thrombosis and multiple organ dysfunction syndrome.
2010,
Pubmed
Gentz,
Medicine and surgery of amphibians.
2007,
Pubmed
Green,
Identification and management of an outbreak of Flavobacterium meningosepticum infection in a colony of South African clawed frogs (Xenopus laevis).
1999,
Pubmed
,
Xenbase
Harris,
Skin microbes on frogs prevent morbidity and mortality caused by a lethal skin fungus.
2009,
Pubmed
Kramer,
Effect of povidone-iodine on wound healing: a review.
1999,
Pubmed
Kurokawa,
Bacterial classification of fish-pathogenic Mycobacterium species by multigene phylogenetic analyses and MALDI Biotyper identification system.
2013,
Pubmed
Lalonde-Robert,
Pharmacologic parameters of MS222 and physiologic changes in frogs (Xenopus laevis) after immersion at anesthetic doses.
2012,
Pubmed
,
Xenbase
Lauer,
Diversity of cutaneous bacteria with antifungal activity isolated from female four-toed salamanders.
2008,
Pubmed
Lay,
MALDI-TOF mass spectrometry of bacteria.
2001,
Pubmed
Lozier,
Effects of four preparations of 0.05% chlorhexidine diacetate on wound healing in dogs.
1992,
Pubmed
Machin,
Amphibian pain and analgesia.
1999,
Pubmed
Martínez-Murcia,
Aeromonas aquariorum sp. nov., isolated from aquaria of ornamental fish.
2008,
Pubmed
Mauel,
Bacterial pathogens isolated from cultured bullfrogs (Rana castesbeiana).
2002,
Pubmed
Olson,
Flavobacterium indologenes infection in leopard frogs.
1992,
Pubmed
Rollins-Smith,
Antimicrobial peptide defenses of the mountain yellow-legged frog (Rana muscosa).
2006,
Pubmed
Sanchez,
Effects of chlorhexidine diacetate and povidone-iodine on wound healing in dogs.
1988,
Pubmed
Schadich,
Pathogenicity of Aeromonas hydrophila, Klebsiella pneumoniae, and Proteus mirabilis to brown tree frogs (Litoria ewingii).
2010,
Pubmed
Schultz,
Housing and husbandry of Xenopus for oocyte production.
2003,
Pubmed
,
Xenbase
Taylor,
Causes of mortality of the Wyoming toad.
1999,
Pubmed
Tuttle,
Evaluation of the gross and histologic reactions to five commonly used suture materials in the skin of the African clawed frog (Xenopus laevis).
2006,
Pubmed
,
Xenbase
Uccelletti,
Anti-Pseudomonas activity of frog skin antimicrobial peptides in a Caenorhabditis elegans infection model: a plausible mode of action in vitro and in vivo.
2010,
Pubmed
Weber,
Outbreaks associated with contaminated antiseptics and disinfectants.
2007,
Pubmed
Weitz,
Chlorhexidine gluconate–impregnated central access catheter dressings as a cause of erosive contact dermatitis: a report of 7 cases.
2013,
Pubmed
