Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Nat Methods
2015 Aug 01;128:743-6. doi: 10.1038/nmeth.3466.
Show Gene links
Show Anatomy links
Multitarget super-resolution microscopy with high-density labeling by exchangeable probes.
Kiuchi T
,
Higuchi M
,
Takamura A
,
Maruoka M
,
Watanabe N
.
???displayArticle.abstract???
We have developed a multitarget super-resolution microscopy technique called image reconstruction by integrating exchangeable single-molecule localization (IRIS). IRIS uses protein fragment-based probes that directly associate with and dissociate from their targets over durations on the order of tens of milliseconds. By integrating single-molecule localization and sequential labeling, IRIS enables unprecedented labeling density along multiple cellular structures. IRIS can be used to discern the area-specific proximity between cytoskeletal components and focal adhesions within a single cell.
Bates,
Multicolor super-resolution imaging with photo-switchable fluorescent probes.
2007, Pubmed
Bates,
Multicolor super-resolution imaging with photo-switchable fluorescent probes.
2007,
Pubmed
Betzig,
Imaging intracellular fluorescent proteins at nanometer resolution.
2006,
Pubmed
Desai,
Kin I kinesins are microtubule-destabilizing enzymes.
1999,
Pubmed
,
Xenbase
Gell,
TIRF microscopy evanescent field calibration using tilted fluorescent microtubules.
2009,
Pubmed
Hell,
Far-field optical nanoscopy.
2007,
Pubmed
Higashida,
Actin polymerization-driven molecular movement of mDia1 in living cells.
2004,
Pubmed
,
Xenbase
Holden,
DAOSTORM: an algorithm for high- density super-resolution microscopy.
2011,
Pubmed
Huang,
Whole-cell 3D STORM reveals interactions between cellular structures with nanometer-scale resolution.
2008,
Pubmed
Huang,
Breaking the diffraction barrier: super-resolution imaging of cells.
2010,
Pubmed
Huang,
Super-resolution fluorescence microscopy.
2009,
Pubmed
Huda,
Microtubule guidance tested through controlled cell geometry.
2012,
Pubmed
Jungmann,
Multiplexed 3D cellular super-resolution imaging with DNA-PAINT and Exchange-PAINT.
2014,
Pubmed
Kanchanawong,
Localization-based super-resolution imaging of cellular structures.
2013,
Pubmed
Kanchanawong,
Nanoscale architecture of integrin-based cell adhesions.
2010,
Pubmed
Mizuno,
Rotational movement of formins evaluated by using single-molecule fluorescence polarization.
2014,
Pubmed
Mizuno,
Rotational movement of the formin mDia1 along the double helical strand of an actin filament.
2011,
Pubmed
Nieuwenhuizen,
Measuring image resolution in optical nanoscopy.
2013,
Pubmed
Riedl,
Lifeact: a versatile marker to visualize F-actin.
2008,
Pubmed
Ries,
A simple, versatile method for GFP-based super-resolution microscopy via nanobodies.
2012,
Pubmed
Rust,
Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM).
2006,
Pubmed
Sauer,
Localization microscopy coming of age: from concepts to biological impact.
2013,
Pubmed
Schoen,
Binding-activated localization microscopy of DNA structures.
2011,
Pubmed
Sharonov,
Wide-field subdiffraction imaging by accumulated binding of diffusing probes.
2006,
Pubmed
Shroff,
Live-cell photoactivated localization microscopy of nanoscale adhesion dynamics.
2008,
Pubmed
Small,
Microtubules meet substrate adhesions to arrange cell polarity.
2003,
Pubmed
Smith,
Interactive, computer-assisted tracking of speckle trajectories in fluorescence microscopy: application to actin polymerization and membrane fusion.
2011,
Pubmed
Tanji,
mDia1 targets v-Src to the cell periphery and facilitates cell transformation, tumorigenesis, and invasion.
2010,
Pubmed
Vale,
Microscopes for fluorimeters: the era of single molecule measurements.
2008,
Pubmed
Watanabe,
Single-molecule speckle analysis of actin filament turnover in lamellipodia.
2002,
Pubmed
,
Xenbase
Yamana,
The Rho-mDia1 pathway regulates cell polarity and focal adhesion turnover in migrating cells through mobilizing Apc and c-Src.
2006,
Pubmed
Yamashiro,
New single-molecule speckle microscopy reveals modification of the retrograde actin flow by focal adhesions at nanometer scales.
2014,
Pubmed
