Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Plant Physiol
2015 Nov 01;1693:2215-29. doi: 10.1104/pp.15.00499.
Show Gene links
Show Anatomy links
Grapevine and Arabidopsis Cation-Chloride Cotransporters Localize to the Golgi and Trans-Golgi Network and Indirectly Influence Long-Distance Ion Transport and Plant Salt Tolerance.
Henderson SW
,
Wege S
,
Qiu J
,
Blackmore DH
,
Walker AR
,
Tyerman SD
,
Walker RR
,
Gilliham M
.
???displayArticle.abstract???
Plant cation-chloride cotransporters (CCCs) have been implicated in conferring salt tolerance. They are predicted to improve shoot salt exclusion by directly catalyzing the retrieval of sodium (Na(+)) and chloride (Cl(-)) ions from the root xylem. We investigated whether grapevine (Vitis vinifera [Vvi]) CCC has a role in salt tolerance by cloning and functionally characterizing the gene from the cultivar Cabernet Sauvignon. Amino acid sequence analysis revealed that VviCCC shares a high degree of similarity with other plant CCCs. A VviCCC-yellow fluorescent protein translational fusion protein localized to the Golgi and the trans-Golgi network and not the plasma membrane when expressed transiently in tobacco (Nicotiana benthamiana) leaves and Arabidopsis (Arabidopsis thaliana) mesophyll protoplasts. AtCCC-green fluorescent protein from Arabidopsis also localized to the Golgi and the trans-Golgi network. In Xenopus laevis oocytes, VviCCC targeted to the plasma membrane, where it catalyzed bumetanide-sensitive (36)Cl(-), (22)Na(+), and (86)Rb(+) uptake, suggesting that VviCCC (like AtCCC) belongs to the Na(+)-K(+)-2Cl(-) cotransporter class of CCCs. Expression of VviCCC in an Arabidopsis ccc knockout mutant abolished the mutant's stunted growth phenotypes and reduced shoot Cl(-) and Na(+) content to wild-type levels after growing plants in 50 mm NaCl. In grapevine roots, VviCCC transcript abundance was not regulated by Cl(-) treatment and was present at similar levels in both the root stele and cortex of three Vitis spp. genotypes that exhibit differential shoot salt exclusion. Our findings indicate that CCC function is conserved between grapevine and Arabidopsis, but neither protein is likely to directly mediate ion transfer with the xylem or have a direct role in salt tolerance.
Arpat,
Functional expression of PHO1 to the Golgi and trans-Golgi network and its role in export of inorganic phosphate.
2012, Pubmed
Arpat,
Functional expression of PHO1 to the Golgi and trans-Golgi network and its role in export of inorganic phosphate.
2012,
Pubmed
Asaoka,
Arabidopsis RABA1 GTPases are involved in transport between the trans-Golgi network and the plasma membrane, and are required for salinity stress tolerance.
2013,
Pubmed
Barberon,
Monoubiquitin-dependent endocytosis of the iron-regulated transporter 1 (IRT1) transporter controls iron uptake in plants.
2011,
Pubmed
Barbier-Brygoo,
Anion channels/transporters in plants: from molecular bases to regulatory networks.
2011,
Pubmed
Bassil,
The Arabidopsis intracellular Na+/H+ antiporters NHX5 and NHX6 are endosome associated and necessary for plant growth and development.
2011,
Pubmed
Beitz,
TEXshade: shading and labeling of multiple sequence alignments using LATEX2 epsilon.
2000,
Pubmed
Benschop,
Quantitative phosphoproteomics of early elicitor signaling in Arabidopsis.
2007,
Pubmed
Brumós,
Cl- homeostasis in includer and excluder citrus rootstocks: transport mechanisms and identification of candidate genes.
2010,
Pubmed
Brumós,
Membrane transporters and carbon metabolism implicated in chloride homeostasis differentiate salt stress responses in tolerant and sensitive Citrus rootstocks.
2009,
Pubmed
Cao,
Reduced expression of a gene encoding a Golgi localized monosaccharide transporter (OsGMST1) confers hypersensitivity to salt in rice (Oryza sativa).
2011,
Pubmed
Clough,
Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana.
1998,
Pubmed
Colmenero-Flores,
Identification and functional characterization of cation-chloride cotransporters in plants.
2007,
Pubmed
,
Xenbase
Conn,
Protocol: optimising hydroponic growth systems for nutritional and physiological analysis of Arabidopsis thaliana and other plants.
2013,
Pubmed
Conn,
Cell-specific vacuolar calcium storage mediated by CAX1 regulates apoplastic calcium concentration, gas exchange, and plant productivity in Arabidopsis.
2011,
Pubmed
Cramer,
Water and salinity stress in grapevines: early and late changes in transcript and metabolite profiles.
2007,
Pubmed
Cubero,
The phosphate transporter PHT4;6 is a determinant of salt tolerance that is localized to the Golgi apparatus of Arabidopsis.
2009,
Pubmed
Curtis,
A gateway cloning vector set for high-throughput functional analysis of genes in planta.
2003,
Pubmed
Drakakaki,
Isolation and proteomic analysis of the SYP61 compartment reveal its role in exocytic trafficking in Arabidopsis.
2012,
Pubmed
Flowers,
Salinity tolerance in halophytes.
2008,
Pubmed
Fricke,
The significance of water co-transport for sustaining transpirational water flow in plants: a quantitative approach.
2015,
Pubmed
Gamba,
Primary structure and functional expression of a cDNA encoding the thiazide-sensitive, electroneutral sodium-chloride cotransporter.
1993,
Pubmed
,
Xenbase
Gamba,
Molecular cloning, primary structure, and characterization of two members of the mammalian electroneutral sodium-(potassium)-chloride cotransporter family expressed in kidney.
1994,
Pubmed
,
Xenbase
Gamba,
Molecular physiology and pathophysiology of electroneutral cation-chloride cotransporters.
2005,
Pubmed
Geldner,
Rapid, combinatorial analysis of membrane compartments in intact plants with a multicolor marker set.
2009,
Pubmed
Gilliham,
The regulation of anion loading to the maize root xylem.
2005,
Pubmed
Gong,
Contrast in chloride exclusion between two grapevine genotypes and its variation in their hybrid progeny.
2011,
Pubmed
Grimplet,
The grapevine gene nomenclature system.
2014,
Pubmed
Groen,
Identification of trans-golgi network proteins in Arabidopsis thaliana root tissue.
2014,
Pubmed
Harling,
A plant cation-chloride co-transporter promoting auxin-independent tobacco protoplast division.
1997,
Pubmed
Harrison,
A rapid and robust method of identifying transformed Arabidopsis thaliana seedlings following floral dip transformation.
2006,
Pubmed
Henderson,
Shoot chloride exclusion and salt tolerance in grapevine is associated with differential ion transporter expression in roots.
2014,
Pubmed
Jha,
Variation in salinity tolerance and shoot sodium accumulation in Arabidopsis ecotypes linked to differences in the natural expression levels of transporters involved in sodium transport.
2010,
Pubmed
Johnson,
Arabidopsis hapless mutations define essential gametophytic functions.
2004,
Pubmed
Kim,
TNO1 is involved in salt tolerance and vacuolar trafficking in Arabidopsis.
2011,
Pubmed
Kong,
Cloning and functional characterization of a cation-chloride cotransporter gene OsCCC1.
2011,
Pubmed
Larkin,
Clustal W and Clustal X version 2.0.
2007,
Pubmed
Maurel,
The vacuolar membrane protein gamma-TIP creates water specific channels in Xenopus oocytes.
1993,
Pubmed
,
Xenbase
Mercado,
Functional comparison of the K+-Cl- cotransporters KCC1 and KCC4.
2000,
Pubmed
,
Xenbase
Molendijk,
A cysteine-rich receptor-like kinase NCRK and a pathogen-induced protein kinase RBK1 are Rop GTPase interactors.
2008,
Pubmed
Munns,
Wheat grain yield on saline soils is improved by an ancestral Na⁺ transporter gene.
2012,
Pubmed
,
Xenbase
Munns,
Mechanisms of salinity tolerance.
2008,
Pubmed
Munns,
Approaches to increasing the salt tolerance of wheat and other cereals.
2006,
Pubmed
Munns,
Salinity tolerance of crops - what is the cost?
2015,
Pubmed
Nelson,
A multicolored set of in vivo organelle markers for co-localization studies in Arabidopsis and other plants.
2007,
Pubmed
Nikolovski,
Putative glycosyltransferases and other plant Golgi apparatus proteins are revealed by LOPIT proteomics.
2012,
Pubmed
Pfaffl,
A new mathematical model for relative quantification in real-time RT-PCR.
2001,
Pubmed
Plett,
Improved salinity tolerance of rice through cell type-specific expression of AtHKT1;1.
2010,
Pubmed
Roxas,
Overexpression of glutathione S-transferase/glutathione peroxidase enhances the growth of transgenic tobacco seedlings during stress.
1997,
Pubmed
Sadowski,
Sub-cellular localization of membrane proteins.
2008,
Pubmed
Shabala,
Learning from halophytes: physiological basis and strategies to improve abiotic stress tolerance in crops.
2013,
Pubmed
Shavrukov,
HvNax3--a locus controlling shoot sodium exclusion derived from wild barley (Hordeum vulgare ssp. spontaneum).
2010,
Pubmed
Subramanian,
A suite of tools and application notes for in vivo protein interaction assays using bioluminescence resonance energy transfer (BRET).
2006,
Pubmed
Suvitayavat,
Characterization of the endogenous Na(+)-K(+)-2Cl- cotransporter in Xenopus oocytes.
1994,
Pubmed
,
Xenbase
Tamura,
MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods.
2011,
Pubmed
Tanz,
SUBA3: a database for integrating experimentation and prediction to define the SUBcellular location of proteins in Arabidopsis.
2013,
Pubmed
Tattersall,
Transcript abundance profiles reveal larger and more complex responses of grapevine to chilling compared to osmotic and salinity stress.
2007,
Pubmed
Teakle,
Mechanisms of Cl(-) transport contributing to salt tolerance.
2010,
Pubmed
Vandesompele,
Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes.
2002,
Pubmed
Vincent,
Proteomic analysis reveals differences between Vitis vinifera L. cv. Chardonnay and cv. Cabernet Sauvignon and their responses to water deficit and salinity.
2007,
Pubmed
Voinnet,
An enhanced transient expression system in plants based on suppression of gene silencing by the p19 protein of tomato bushy stunt virus.
2003,
Pubmed
Wege,
Expression of the mammalian Xenotropic Polytropic Virus Receptor 1 (XPR1) in tobacco leaves leads to phosphate export.
2014,
Pubmed
Wegner,
Root pressure and beyond: energetically uphill water transport into xylem vessels?
2014,
Pubmed
Whiteman,
Identification of novel proteins and phosphorylation sites in a tonoplast enriched membrane fraction of Arabidopsis thaliana.
2008,
Pubmed
Whiteman,
A proteomic and phosphoproteomic analysis of Oryza sativa plasma membrane and vacuolar membrane.
2008,
Pubmed
Wu,
Tape-Arabidopsis Sandwich - a simpler Arabidopsis protoplast isolation method.
2009,
Pubmed
Zeuthen,
Cotransport of water by Na⁺-K⁺-2Cl⁻ cotransporters expressed in Xenopus oocytes: NKCC1 versus NKCC2.
2012,
Pubmed
,
Xenbase
