Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Plant Signal Behav
2016 Jan 01;112:e1071751. doi: 10.1080/15592324.2015.1071751.
Show Gene links
Show Anatomy links
Phosphorylation at serine 52 and 635 does not alter the transport properties of glucosinolate transporter AtGTR1.
Jørgensen ME
,
Olsen CE
,
Halkier BA
,
Nour-Eldin HH
.
???displayArticle.abstract???
Little is known about how plants regulate transporters of defense compounds. In A. thaliana, glucosinolates are transported between tissues by NPF2.10 (AtGTR1) and NPF2.11 (AtGTR2). Mining of the PhosPhat4.0 database showed two cytosol exposed phosphorylation sites for AtGTR1 and one membrane-buried phosphorylation site for AtGTR2. In this study, we investigate whether mutation of the two potential regulatory sites of AtGTR1 affected transport of glucosinolates in Xenopus oocytes. Characterization of AtGTR1 phosphorylation mutants showed that phosphorylation of AtGTR1 - at the two reported phosphorylation sites - is not directly involved in regulating AtGTR1 transport activity. We hypothesize a role for AtGTR1-phosphorylation in regulating protein-protein interactions.
Andersen,
Integration of biosynthesis and long-distance transport establish organ-specific glucosinolate profiles in vegetative Arabidopsis.
2013, Pubmed,
Xenbase
Andersen,
Integration of biosynthesis and long-distance transport establish organ-specific glucosinolate profiles in vegetative Arabidopsis.
2013,
Pubmed
,
Xenbase
Benschop,
Quantitative phosphoproteomics of early elicitor signaling in Arabidopsis.
2007,
Pubmed
Biasini,
SWISS-MODEL: modelling protein tertiary and quaternary structure using evolutionary information.
2014,
Pubmed
Boursiac,
Early effects of salinity on water transport in Arabidopsis roots. Molecular and cellular features of aquaporin expression.
2005,
Pubmed
Chen,
Comparative analysis of phytohormone-responsive phosphoproteins in Arabidopsis thaliana using TiO2-phosphopeptide enrichment and mass accuracy precursor alignment.
2010,
Pubmed
Cuthbertson,
Transmembrane helix prediction: a comparative evaluation and analysis.
2005,
Pubmed
Fuglsang,
Binding of 14-3-3 protein to the plasma membrane H(+)-ATPase AHA2 involves the three C-terminal residues Tyr(946)-Thr-Val and requires phosphorylation of Thr(947).
1999,
Pubmed
Geiger,
Stomatal closure by fast abscisic acid signaling is mediated by the guard cell anion channel SLAH3 and the receptor RCAR1.
2011,
Pubmed
,
Xenbase
Geiger,
Activity of guard cell anion channel SLAC1 is controlled by drought-stress signaling kinase-phosphatase pair.
2009,
Pubmed
,
Xenbase
Geiger,
Guard cell anion channel SLAC1 is regulated by CDPK protein kinases with distinct Ca2+ affinities.
2010,
Pubmed
,
Xenbase
Geu-Flores,
USER fusion: a rapid and efficient method for simultaneous fusion and cloning of multiple PCR products.
2007,
Pubmed
Halkier,
Biology and biochemistry of glucosinolates.
2006,
Pubmed
Heazlewood,
PhosPhAt: a database of phosphorylation sites in Arabidopsis thaliana and a plant-specific phosphorylation site predictor.
2008,
Pubmed
Hildreth,
Tobacco nicotine uptake permease (NUP1) affects alkaloid metabolism.
2011,
Pubmed
Jørgensen,
Transport of defense compounds from source to sink: lessons learned from glucosinolates.
2015,
Pubmed
Kelm,
iMembrane: homology-based membrane-insertion of proteins.
2009,
Pubmed
Latz,
Salt stress triggers phosphorylation of the Arabidopsis vacuolar K+ channel TPK1 by calcium-dependent protein kinases (CDPKs).
2013,
Pubmed
Latz,
TPK1, a Ca(2+)-regulated Arabidopsis vacuole two-pore K(+) channel is activated by 14-3-3 proteins.
2007,
Pubmed
Li,
An Abscisic Acid-Activated and Calcium-Independent Protein Kinase from Guard Cells of Fava Bean.
1996,
Pubmed
Li,
Evidence for protein phosphatase 1 and 2A regulation of K+ channels in two types of leaf cells.
1994,
Pubmed
Liu,
Switching between the two action modes of the dual-affinity nitrate transporter CHL1 by phosphorylation.
2003,
Pubmed
,
Xenbase
Léran,
A unified nomenclature of NITRATE TRANSPORTER 1/PEPTIDE TRANSPORTER family members in plants.
2014,
Pubmed
Morita,
Vacuolar transport of nicotine is mediated by a multidrug and toxic compound extrusion (MATE) transporter in Nicotiana tabacum.
2009,
Pubmed
Nour-Eldin,
Advancing uracil-excision based cloning towards an ideal technique for cloning PCR fragments.
2006,
Pubmed
Nour-Eldin,
NRT/PTR transporters are essential for translocation of glucosinolate defence compounds to seeds.
2012,
Pubmed
,
Xenbase
Nühse,
Phosphoproteomics of the Arabidopsis plasma membrane and a new phosphorylation site database.
2004,
Pubmed
Nühse,
Quantitative phosphoproteomic analysis of plasma membrane proteins reveals regulatory mechanisms of plant innate immune responses.
2007,
Pubmed
Parker,
Molecular basis of nitrate uptake by the plant nitrate transporter NRT1.1.
2014,
Pubmed
Prak,
Multiple phosphorylations in the C-terminal tail of plant plasma membrane aquaporins: role in subcellular trafficking of AtPIP2;1 in response to salt stress.
2008,
Pubmed
Shitan,
Characterization of Coptis japonica CjABCB2, an ATP-binding cassette protein involved in alkaloid transport.
2013,
Pubmed
Shitan,
Involvement of CjMDR1, a plant multidrug-resistance-type ATP-binding cassette protein, in alkaloid transport in Coptis japonica.
2003,
Pubmed
,
Xenbase
Shoji,
Multidrug and toxic compound extrusion-type transporters implicated in vacuolar sequestration of nicotine in tobacco roots.
2009,
Pubmed
Sun,
Crystal structure of the plant dual-affinity nitrate transporter NRT1.1.
2014,
Pubmed
Törnroth-Horsefield,
Structural mechanism of plant aquaporin gating.
2006,
Pubmed
Zourelidou,
Auxin efflux by PIN-FORMED proteins is activated by two different protein kinases, D6 PROTEIN KINASE and PINOID.
2014,
Pubmed
,
Xenbase
Züst,
Natural enemies drive geographic variation in plant defenses.
2012,
Pubmed
de Beer,
PDBsum additions.
2014,
Pubmed
