Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Proc Natl Acad Sci U S A
2015 Apr 21;11216:5237-42. doi: 10.1073/pnas.1500953112.
Show Gene links
Show Anatomy links
Cadmium-cysteine coordination in the BK inner pore region and its structural and functional implications.
Zhou Y
,
Xia XM
,
Lingle CJ
.
???displayArticle.abstract???
To probe structure and gating-associated conformational changes in BK-type potassium (BK) channels, we examined consequences of Cd(2+) coordination with cysteines introduced at two positions in the BK inner pore. At V319C, the equivalent of valine in the conserved Kv proline-valine-proline (PVP) motif, Cd(2+) forms intrasubunit coordination with a native glutamate E321, which would place the side chains of V319C and E321 much closer together than observed in voltage-dependent K(+) (Kv) channel structures, requiring that the proline between V319C and E321 introduces a kink in the BK S6 inner helix sharper than that observed in Kv channel structures. At inner pore position A316C, Cd(2+) binds with modest state dependence, suggesting the absence of an ion permeation gate at the cytosolic side of BK channel. These results highlight fundamental structural differences between BK and Kv channels in their inner pore region, which likely underlie differences in voltage-dependent gating between these channels.
Bezanilla,
How membrane proteins sense voltage.
2008, Pubmed
Bezanilla,
How membrane proteins sense voltage.
2008,
Pubmed
Chen,
Charge substitution for a deep-pore residue reveals structural dynamics during BK channel gating.
2011,
Pubmed
Chen,
BK channel opening involves side-chain reorientation of multiple deep-pore residues.
2014,
Pubmed
Contreras,
Gating at the selectivity filter in cyclic nucleotide-gated channels.
2008,
Pubmed
Eckhardt,
Structural characterization of a beta-turn mimic within a protein-protein interface.
2010,
Pubmed
Hamill,
Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches.
1981,
Pubmed
Horrigan,
Coupling between voltage sensor activation, Ca2+ binding and channel opening in large conductance (BK) potassium channels.
2002,
Pubmed
,
Xenbase
Horrigan,
Allosteric voltage gating of potassium channels I. Mslo ionic currents in the absence of Ca(2+).
1999,
Pubmed
,
Xenbase
Hosfield,
Structural basis for bisphosphonate-mediated inhibition of isoprenoid biosynthesis.
2004,
Pubmed
Hutchinson,
A revised set of potentials for beta-turn formation in proteins.
1994,
Pubmed
Jakob,
Elimination of a cis-proline-containing loop and turn optimization stabilizes a protein and accelerates its folding.
2010,
Pubmed
Li,
State-dependent block of BK channels by synthesized shaker ball peptides.
2006,
Pubmed
,
Xenbase
Liu,
Gated access to the pore of a voltage-dependent K+ channel.
1997,
Pubmed
Long,
Voltage sensor of Kv1.2: structural basis of electromechanical coupling.
2005,
Pubmed
Long,
Crystal structure of a mammalian voltage-dependent Shaker family K+ channel.
2005,
Pubmed
Long,
Atomic structure of a voltage-dependent K+ channel in a lipid membrane-like environment.
2007,
Pubmed
Lu,
Coupling between voltage sensors and activation gate in voltage-gated K+ channels.
2002,
Pubmed
,
Xenbase
Lu,
Ion conduction pore is conserved among potassium channels.
2001,
Pubmed
Niu,
Stepwise contribution of each subunit to the cooperative activation of BK channels by Ca2+.
2002,
Pubmed
Puljung,
Labeling of specific cysteines in proteins using reversible metal protection.
2011,
Pubmed
,
Xenbase
Rulísek,
Coordination geometries of selected transition metal ions (Co2+, Ni2+, Cu2+, Zn2+, Cd2+, and Hg2+) in metalloproteins.
1998,
Pubmed
Salkoff,
High-conductance potassium channels of the SLO family.
2006,
Pubmed
Shen,
Tetraethylammonium block of Slowpoke calcium-activated potassium channels expressed in Xenopus oocytes: evidence for tetrameric channel formation.
1994,
Pubmed
,
Xenbase
Shi,
Mechanism of magnesium activation of calcium-activated potassium channels.
2002,
Pubmed
Tang,
Closed-channel block of BK potassium channels by bbTBA requires partial activation.
2009,
Pubmed
,
Xenbase
Thompson,
Selectivity filter gating in large-conductance Ca(2+)-activated K+ channels.
2012,
Pubmed
,
Xenbase
Unno,
New role of flavin as a general acid-base catalyst with no redox function in type 2 isopentenyl-diphosphate isomerase.
2009,
Pubmed
Webster,
Intracellular gate opening in Shaker K+ channels defined by high-affinity metal bridges.
2004,
Pubmed
Wilkens,
State-independent block of BK channels by an intracellular quaternary ammonium.
2006,
Pubmed
,
Xenbase
Xia,
Multiple regulatory sites in large-conductance calcium-activated potassium channels.
2002,
Pubmed
Zeng,
Gating properties conferred on BK channels by the beta3b auxiliary subunit in the absence of its NH(2)- and COOH termini.
2001,
Pubmed
,
Xenbase
Zeng,
Divalent cation sensitivity of BK channel activation supports the existence of three distinct binding sites.
2005,
Pubmed
,
Xenbase
Zhang,
Allosteric regulation of BK channel gating by Ca(2+) and Mg(2+) through a nonselective, low affinity divalent cation site.
2001,
Pubmed
,
Xenbase
Zhang,
Cysteine modification alters voltage- and Ca(2+)-dependent gating of large conductance (BK) potassium channels.
2005,
Pubmed
Zhou,
Cysteine scanning and modification reveal major differences between BK channels and Kv channels in the inner pore region.
2011,
Pubmed
,
Xenbase
del Camino,
Tight steric closure at the intracellular activation gate of a voltage-gated K(+) channel.
2001,
Pubmed
