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Channels (Austin)
2015 Jan 01;93:121-8. doi: 10.1080/19336950.2015.1036205.
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PKC and AMPK regulation of Kv1.5 potassium channels.
Andersen MN
,
Skibsbye L
,
Tang C
,
Petersen F
,
MacAulay N
,
Rasmussen HB
,
Jespersen T
.
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The voltage-gated Kv1.5 potassium channel, conducting the ultra-rapid rectifier K(+) current (IKur), is regulated through several pathways. Here we investigate if Kv1.5 surface expression is controlled by the 2 kinases PKC and AMPK, using Xenopus oocytes, MDCK cells and atrial derived HL-1 cells. By confocal microscopy combined with electrophysiology we demonstrate that PKC activation reduces Kv1.5 current, through a decrease in membrane expressed channels. AMPK activation was found to decrease the membrane expression in MDCK cells, but not in HL-1 cells and was furthermore shown to be dependent on co-expression of Nedd4-2 in Xenopus oocytes. These results indicate that Kv1.5 channels are regulated by both kinases, although through different molecular mechanisms in different cell systems.
Adda,
Expression and function of voltage-dependent potassium channel genes in human airway smooth muscle.
1996, Pubmed
Adda,
Expression and function of voltage-dependent potassium channel genes in human airway smooth muscle.
1996,
Pubmed
Alesutan,
Inhibition of the heterotetrameric K+ channel KCNQ1/KCNE1 by the AMP-activated protein kinase.
2011,
Pubmed
,
Xenbase
Alzamora,
AMP-activated protein kinase inhibits KCNQ1 channels through regulation of the ubiquitin ligase Nedd4-2 in renal epithelial cells.
2010,
Pubmed
,
Xenbase
Andersen,
Kv7.1 surface expression is regulated by epithelial cell polarization.
2011,
Pubmed
Andersen,
AMPK: A regulator of ion channels.
2012,
Pubmed
Andersen,
A phosphoinositide 3-kinase (PI3K)-serum- and glucocorticoid-inducible kinase 1 (SGK1) pathway promotes Kv7.1 channel surface expression by inhibiting Nedd4-2 protein.
2013,
Pubmed
Andersen,
AMP-activated protein kinase downregulates Kv7.1 cell surface expression.
2012,
Pubmed
,
Xenbase
Bielanska,
Voltage-dependent potassium channels Kv1.3 and Kv1.5 in human cancer.
2009,
Pubmed
Bielanska,
Voltage-dependent potassium channels Kv1.3 and Kv1.5 in human fetus.
2010,
Pubmed
Boehmer,
Modulation of the voltage-gated potassium channel Kv1.5 by the SGK1 protein kinase involves inhibition of channel ubiquitination.
2008,
Pubmed
,
Xenbase
Brouillette,
Functional properties of K+ currents in adult mouse ventricular myocytes.
2004,
Pubmed
Christophersen,
Genetic variation in KCNA5: impact on the atrial-specific potassium current IKur in patients with lone atrial fibrillation.
2013,
Pubmed
Claycomb,
HL-1 cells: a cardiac muscle cell line that contracts and retains phenotypic characteristics of the adult cardiomyocyte.
1998,
Pubmed
David,
Protein kinase C (PKC) activity regulates functional effects of Kvβ1.3 subunit on KV1.5 channels: identification of a cardiac Kv1.5 channelosome.
2012,
Pubmed
Fischer,
Isoenzyme-specific regulation of cardiac Kv1.5/Kvβ1.2 ion channel complex by protein kinase C: central role of PKCβII.
2014,
Pubmed
,
Xenbase
Ford,
Human electrophysiological and pharmacological properties of XEN-D0101: a novel atrial-selective Kv1.5/IKur inhibitor.
2013,
Pubmed
Grunnet,
Apamin interacts with all subtypes of cloned small-conductance Ca2+-activated K+ channels.
2001,
Pubmed
,
Xenbase
Jespersen,
The KCNQ1 potassium channel is down-regulated by ubiquitylating enzymes of the Nedd4/Nedd4-like family.
2007,
Pubmed
Kang,
Aging and muscle fiber type alter K+ channel contributions to the myogenic response in skeletal muscle arterioles.
2009,
Pubmed
Kwak,
Phosphorylation is required for alteration of kv1.5 K(+) channel function by the Kvbeta1.3 subunit.
1999,
Pubmed
Kwak,
Protein kinase A phosphorylation alters Kvbeta1.3 subunit-mediated inactivation of the Kv1.5 potassium channel.
1999,
Pubmed
,
Xenbase
Lang,
On the putative purpose of AMPK sensitive Kv1.5 K(+) channel regulation.
2015,
Pubmed
Li,
KChIP2 modulates the cell surface expression of Kv 1.5-encoded K(+) channels.
2005,
Pubmed
Lundby,
Effect of the I(to) activator NS5806 on cloned K(V)4 channels depends on the accessory protein KChIP2.
2010,
Pubmed
,
Xenbase
MacDonald,
Voltage-dependent K(+) channels in pancreatic beta cells: role, regulation and potential as therapeutic targets.
2003,
Pubmed
Mia,
Downregulation of Kv1.5 K channels by the AMP-activated protein kinase.
2012,
Pubmed
,
Xenbase
Olson,
Kv1.5 channelopathy due to KCNA5 loss-of-function mutation causes human atrial fibrillation.
2006,
Pubmed
Pang,
Small molecule antagonizes autoinhibition and activates AMP-activated protein kinase in cells.
2008,
Pubmed
Plane,
Heteromultimeric Kv1 channels contribute to myogenic control of arterial diameter.
2005,
Pubmed
Ravens,
Ultra-rapid delayed rectifier channels: molecular basis and therapeutic implications.
2011,
Pubmed
Thorneloe,
Molecular composition of 4-aminopyridine-sensitive voltage-gated K(+) channels of vascular smooth muscle.
2001,
Pubmed
Uebele,
Functional differences in Kv1.5 currents expressed in mammalian cell lines are due to the presence of endogenous Kv beta 2.1 subunits.
1996,
Pubmed
Uebele,
Distinct domains of the voltage-gated K+ channel Kv beta 1.3 beta-subunit affect voltage-dependent gating.
1998,
Pubmed
,
Xenbase
Wang,
Sustained depolarization-induced outward current in human atrial myocytes. Evidence for a novel delayed rectifier K+ current similar to Kv1.5 cloned channel currents.
1993,
Pubmed
Williams,
Modulation of the human Kv1.5 channel by protein kinase C activation: role of the Kvbeta1.2 subunit.
2002,
Pubmed