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Plant Mol Biol
2015 May 01;881-2:165-76. doi: 10.1007/s11103-015-0315-0.
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Iron deficiency regulated OsOPT7 is essential for iron homeostasis in rice.
Bashir K
,
Ishimaru Y
,
Itai RN
,
Senoura T
,
Takahashi M
,
An G
,
Oikawa T
,
Ueda M
,
Sato A
,
Uozumi N
,
Nakanishi H
,
Nishizawa NK
.
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The molecular mechanism of iron (Fe) uptake and transport in plants are well-characterized; however, many components of Fe homeostasis remain unclear. We cloned iron-deficiency-regulated oligopeptide transporter 7 (OsOPT7) from rice. OsOPT7 localized to the plasma membrane and did not transport Fe(III)-DMA or Fe(II)-NA and GSH in Xenopus laevis oocytes. Furthermore OsOPT7 did not complement the growth of yeast fet3fet4 mutant. OsOPT7 was specifically upregulated in response to Fe-deficiency. Promoter GUS analysis revealed that OsOPT7 expresses in root tips, root vascular tissue and shoots as well as during seed development. Microarray analysis of OsOPT7 knockout 1 (opt7-1) revealed the upregulation of Fe-deficiency-responsive genes in plants grown under Fe-sufficient conditions, despite the high Fe and ferritin concentrations in shoot tissue indicating that Fe may not be available for physiological functions. Plants overexpressing OsOPT7 do not exhibit any phenotype and do not accumulate more Fe compared to wild type plants. These results indicate that OsOPT7 may be involved in Fe transport in rice.
Bashir,
Transcriptomic analysis of rice in response to iron deficiency and excess.
2014, Pubmed
Bashir,
Transcriptomic analysis of rice in response to iron deficiency and excess.
2014,
Pubmed
Bashir,
The road to micronutrient biofortification of rice: progress and prospects.
2013,
Pubmed
Bashir,
Deoxymugineic Acid synthase: a gene important for fe-acquisition and homeostasis.
2006,
Pubmed
Bashir,
The rice mitochondrial iron transporter is essential for plant growth.
2011,
Pubmed
Bashir,
Exploiting new tools for iron bio-fortification of rice.
2013,
Pubmed
Bashir,
Identification and characterization of the major mitochondrial Fe transporter in rice.
2011,
Pubmed
Bashir,
Cloning and characterization of deoxymugineic acid synthase genes from graminaceous plants.
2006,
Pubmed
Bashir,
The knockdown of OsVIT2 and MIT affects iron localization in rice seed.
2013,
Pubmed
Bashir,
Expression and enzyme activity of glutathione reductase is upregulated by Fe-deficiency in graminaceous plants.
2007,
Pubmed
Briat,
New insights into ferritin synthesis and function highlight a link between iron homeostasis and oxidative stress in plants.
2010,
Pubmed
Curie,
Maize yellow stripe1 encodes a membrane protein directly involved in Fe(III) uptake.
2001,
Pubmed
Engler-Blum,
Reduction of background problems in nonradioactive northern and Southern blot analyses enables higher sensitivity than 32P-based hybridizations.
1993,
Pubmed
Guerinot,
Iron: Nutritious, Noxious, and Not Readily Available.
1994,
Pubmed
Harrison,
The ferritins: molecular properties, iron storage function and cellular regulation.
1996,
Pubmed
Inoue,
Three rice nicotianamine synthase genes, OsNAS1, OsNAS2, and OsNAS3 are expressed in cells involved in long-distance transport of iron and differentially regulated by iron.
2003,
Pubmed
Inoue,
Rice OsYSL15 is an iron-regulated iron(III)-deoxymugineic acid transporter expressed in the roots and is essential for iron uptake in early growth of the seedlings.
2009,
Pubmed
,
Xenbase
Ishimaru,
The role of rice phenolics efflux transporter in solubilizing apoplasmic iron.
2011,
Pubmed
,
Xenbase
Ishimaru,
Rice metal-nicotianamine transporter, OsYSL2, is required for the long-distance transport of iron and manganese.
2010,
Pubmed
Ishimaru,
Mutational reconstructed ferric chelate reductase confers enhanced tolerance in rice to iron deficiency in calcareous soil.
2007,
Pubmed
Ishimaru,
Rice plants take up iron as an Fe3+-phytosiderophore and as Fe2+.
2006,
Pubmed
Ishimaru,
Rice-specific mitochondrial iron-regulated gene (MIR) plays an important role in iron homeostasis.
2009,
Pubmed
Ishimaru,
A rice phenolic efflux transporter is essential for solubilizing precipitated apoplasmic iron in the plant stele.
2011,
Pubmed
Kakei,
OsYSL16 plays a role in the allocation of iron.
2012,
Pubmed
Karimi,
GATEWAY vectors for Agrobacterium-mediated plant transformation.
2002,
Pubmed
Kato,
Evidence in support of a four transmembrane-pore-transmembrane topology model for the Arabidopsis thaliana Na+/K+ translocating AtHKT1 protein, a member of the superfamily of K+ transporters.
2001,
Pubmed
,
Xenbase
Kim,
Localization of iron in Arabidopsis seed requires the vacuolar membrane transporter VIT1.
2006,
Pubmed
Kobayashi,
Iron uptake, translocation, and regulation in higher plants.
2012,
Pubmed
Koike,
OsYSL2 is a rice metal-nicotianamine transporter that is regulated by iron and expressed in the phloem.
2004,
Pubmed
,
Xenbase
Lee,
Disruption of OsYSL15 leads to iron inefficiency in rice plants.
2009,
Pubmed
Lescure,
Ferritin gene transcription is regulated by iron in soybean cell cultures.
1991,
Pubmed
Lingam,
Interaction between the bHLH transcription factor FIT and ETHYLENE INSENSITIVE3/ETHYLENE INSENSITIVE3-LIKE1 reveals molecular linkage between the regulation of iron acquisition and ethylene signaling in Arabidopsis.
2011,
Pubmed
Liu,
Evolutionary expansion and functional diversification of oligopeptide transporter gene family in rice.
2012,
Pubmed
Mendoza-Cózatl,
OPT3 is a component of the iron-signaling network between leaves and roots and misregulation of OPT3 leads to an over-accumulation of cadmium in seeds.
2014,
Pubmed
Mori,
Iron acquisition by plants.
1999,
Pubmed
Nishiyama,
Identification of Zn-nicotianamine and Fe-2'-Deoxymugineic acid in the phloem sap from rice plants (Oryza sativa L.).
2012,
Pubmed
Nozoye,
Nicotianamine synthase 2 localizes to the vesicles of iron-deficient rice roots, and its mutation in the YXXφ or LL motif causes the disruption of vesicle formation or movement in rice.
2014,
Pubmed
Nozoye,
Phytosiderophore efflux transporters are crucial for iron acquisition in graminaceous plants.
2011,
Pubmed
,
Xenbase
Nozoye,
Rice nicotianamine synthase localizes to particular vesicles for proper function.
2014,
Pubmed
Ogo,
Isolation and characterization of IRO2, a novel iron-regulated bHLH transcription factor in graminaceous plants.
2006,
Pubmed
Ogo,
The rice bHLH protein OsIRO2 is an essential regulator of the genes involved in Fe uptake under Fe-deficient conditions.
2007,
Pubmed
Ryu,
OsMADS50 and OsMADS56 function antagonistically in regulating long day (LD)-dependent flowering in rice.
2009,
Pubmed
Stacey,
The Arabidopsis AtOPT3 protein functions in metal homeostasis and movement of iron to developing seeds.
2008,
Pubmed
Stacey,
Expression analyses of Arabidopsis oligopeptide transporters during seed germination, vegetative growth and reproduction.
2006,
Pubmed
Van Wuytswinkel,
Iron homeostasis alteration in transgenic tobacco overexpressing ferritin.
1999,
Pubmed
Wiles,
Transmembrane domain prediction and consensus sequence identification of the oligopeptide transport family.
2006,
Pubmed
Yamaji,
The node, a hub for mineral nutrient distribution in graminaceous plants.
2014,
Pubmed
Yokosho,
OsFRDL1 is a citrate transporter required for efficient translocation of iron in rice.
2009,
Pubmed
,
Xenbase
Zhai,
OPT3 Is a Phloem-Specific Iron Transporter That Is Essential for Systemic Iron Signaling and Redistribution of Iron and Cadmium in Arabidopsis.
2014,
Pubmed
Zhang,
Vacuolar membrane transporters OsVIT1 and OsVIT2 modulate iron translocation between flag leaves and seeds in rice.
2012,
Pubmed
Zhang,
A novel family of transporters mediating the transport of glutathione derivatives in plants.
2004,
Pubmed
